Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis
Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for el...
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crroyalsociety:10.1098/rsos.160106 2024-09-15T17:46:01+00:00 Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis Tsuji, Masaharu a Research Organization of Information and Systems, Japan (ROIS), grant for Young Scientists JSPS Grant-in-Aid for Research Activity Start-up Grant 2016 http://dx.doi.org/10.1098/rsos.160106 https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160106 https://royalsocietypublishing.org/doi/full-xml/10.1098/rsos.160106 en eng The Royal Society https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ Royal Society Open Science volume 3, issue 7, page 160106 ISSN 2054-5703 journal-article 2016 crroyalsociety https://doi.org/10.1098/rsos.160106 2024-07-29T04:23:18Z Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not. I analysed the metabolite responses elicited by cold stress in these two M. blollopis strains by using capillary electrophoresis–time-of-flight mass spectrometry. M. blollopis SK-4, which grew well under subzero temperatures, accumulated high levels of TCA-cycle metabolites, lactic acid, aromatic amino acids and polyamines in response to cold shock. Polyamines are recognized to function in cell-growth and developmental processes, and aromatic amino acids are also known to improve cell growth at low temperatures. By contrast, in TKG1-2, which did not grow efficiently, cold stress strongly induced the metabolites of the TCA cycle, but other metabolites were not highly accumulated in the cell. Thus, these differences in metabolite responses could contribute to the distinct abilities of SK-4 and TKG1-2 cells to grow under subzero temperature conditions. Article in Journal/Newspaper Antarc* Antarctic The Royal Society Royal Society Open Science 3 7 160106 |
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Open Polar |
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The Royal Society |
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crroyalsociety |
language |
English |
description |
Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not. I analysed the metabolite responses elicited by cold stress in these two M. blollopis strains by using capillary electrophoresis–time-of-flight mass spectrometry. M. blollopis SK-4, which grew well under subzero temperatures, accumulated high levels of TCA-cycle metabolites, lactic acid, aromatic amino acids and polyamines in response to cold shock. Polyamines are recognized to function in cell-growth and developmental processes, and aromatic amino acids are also known to improve cell growth at low temperatures. By contrast, in TKG1-2, which did not grow efficiently, cold stress strongly induced the metabolites of the TCA cycle, but other metabolites were not highly accumulated in the cell. Thus, these differences in metabolite responses could contribute to the distinct abilities of SK-4 and TKG1-2 cells to grow under subzero temperature conditions. |
author2 |
a Research Organization of Information and Systems, Japan (ROIS), grant for Young Scientists JSPS Grant-in-Aid for Research Activity Start-up Grant |
format |
Article in Journal/Newspaper |
author |
Tsuji, Masaharu |
spellingShingle |
Tsuji, Masaharu Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
author_facet |
Tsuji, Masaharu |
author_sort |
Tsuji, Masaharu |
title |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_short |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_full |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_fullStr |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_full_unstemmed |
Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_sort |
cold-stress responses in the antarctic basidiomycetous yeast mrakia blollopis |
publisher |
The Royal Society |
publishDate |
2016 |
url |
http://dx.doi.org/10.1098/rsos.160106 https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160106 https://royalsocietypublishing.org/doi/full-xml/10.1098/rsos.160106 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Royal Society Open Science volume 3, issue 7, page 160106 ISSN 2054-5703 |
op_rights |
https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ |
op_doi |
https://doi.org/10.1098/rsos.160106 |
container_title |
Royal Society Open Science |
container_volume |
3 |
container_issue |
7 |
container_start_page |
160106 |
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1810493960073773056 |