RAD sequencing and genomic simulations resolve hybrid origins within North American Canis
Top predators are disappearing worldwide, significantly changing ecosystems that depend on top-down regulation. Conflict with humans remains the primary roadblock for large carnivore conservation, but for the eastern wolf ( Canis lycaon ), disagreement over its evolutionary origins presents a signif...
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crroyalsociety:10.1098/rsbl.2015.0303 2024-06-02T08:05:02+00:00 RAD sequencing and genomic simulations resolve hybrid origins within North American Canis Rutledge, L. Y. Devillard, S. Boone, J. Q. Hohenlohe, P. A. White, B. N. 2015 http://dx.doi.org/10.1098/rsbl.2015.0303 https://royalsocietypublishing.org/doi/pdf/10.1098/rsbl.2015.0303 https://royalsocietypublishing.org/doi/full-xml/10.1098/rsbl.2015.0303 en eng The Royal Society https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ Biology Letters volume 11, issue 7, page 20150303 ISSN 1744-9561 1744-957X journal-article 2015 crroyalsociety https://doi.org/10.1098/rsbl.2015.0303 2024-05-07T14:16:14Z Top predators are disappearing worldwide, significantly changing ecosystems that depend on top-down regulation. Conflict with humans remains the primary roadblock for large carnivore conservation, but for the eastern wolf ( Canis lycaon ), disagreement over its evolutionary origins presents a significant barrier to conservation in Canada and has impeded protection for grey wolves ( Canis lupus ) in the USA. Here, we use 127 235 single-nucleotide polymorphisms (SNPs) identified from restriction-site associated DNA sequencing (RAD-seq) of wolves and coyotes, in combination with genomic simulations, to test hypotheses of hybrid origins of Canis types in eastern North America. A principal components analysis revealed no evidence to support eastern wolves, or any other Canis type, as the product of grey wolf × western coyote hybridization. In contrast, simulations that included eastern wolves as a distinct taxon clarified the hybrid origins of Great Lakes-boreal wolves and eastern coyotes. Our results support the eastern wolf as a distinct genomic cluster in North America and help resolve hybrid origins of Great Lakes wolves and eastern coyotes. The data provide timely information that will shed new light on the debate over wolf conservation in eastern North America. Article in Journal/Newspaper Canis lupus The Royal Society Canada Biology Letters 11 7 20150303 |
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The Royal Society |
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crroyalsociety |
language |
English |
description |
Top predators are disappearing worldwide, significantly changing ecosystems that depend on top-down regulation. Conflict with humans remains the primary roadblock for large carnivore conservation, but for the eastern wolf ( Canis lycaon ), disagreement over its evolutionary origins presents a significant barrier to conservation in Canada and has impeded protection for grey wolves ( Canis lupus ) in the USA. Here, we use 127 235 single-nucleotide polymorphisms (SNPs) identified from restriction-site associated DNA sequencing (RAD-seq) of wolves and coyotes, in combination with genomic simulations, to test hypotheses of hybrid origins of Canis types in eastern North America. A principal components analysis revealed no evidence to support eastern wolves, or any other Canis type, as the product of grey wolf × western coyote hybridization. In contrast, simulations that included eastern wolves as a distinct taxon clarified the hybrid origins of Great Lakes-boreal wolves and eastern coyotes. Our results support the eastern wolf as a distinct genomic cluster in North America and help resolve hybrid origins of Great Lakes wolves and eastern coyotes. The data provide timely information that will shed new light on the debate over wolf conservation in eastern North America. |
format |
Article in Journal/Newspaper |
author |
Rutledge, L. Y. Devillard, S. Boone, J. Q. Hohenlohe, P. A. White, B. N. |
spellingShingle |
Rutledge, L. Y. Devillard, S. Boone, J. Q. Hohenlohe, P. A. White, B. N. RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
author_facet |
Rutledge, L. Y. Devillard, S. Boone, J. Q. Hohenlohe, P. A. White, B. N. |
author_sort |
Rutledge, L. Y. |
title |
RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
title_short |
RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
title_full |
RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
title_fullStr |
RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
title_full_unstemmed |
RAD sequencing and genomic simulations resolve hybrid origins within North American Canis |
title_sort |
rad sequencing and genomic simulations resolve hybrid origins within north american canis |
publisher |
The Royal Society |
publishDate |
2015 |
url |
http://dx.doi.org/10.1098/rsbl.2015.0303 https://royalsocietypublishing.org/doi/pdf/10.1098/rsbl.2015.0303 https://royalsocietypublishing.org/doi/full-xml/10.1098/rsbl.2015.0303 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Biology Letters volume 11, issue 7, page 20150303 ISSN 1744-9561 1744-957X |
op_rights |
https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ |
op_doi |
https://doi.org/10.1098/rsbl.2015.0303 |
container_title |
Biology Letters |
container_volume |
11 |
container_issue |
7 |
container_start_page |
20150303 |
_version_ |
1800749773964181504 |