T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo
We recently described a novel way to isolate populations of antigen-reactive CD4+ T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS® sorting by CD4high expression. Phenotypic, FACS®, functional, antibody inhibition, and ma...
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Online Access: | http://dx.doi.org/10.1084/jem.192.12.1719 https://rupress.org/jem/article-pdf/192/12/1719/1699640/990972.pdf |
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crrockefelleruni:10.1084/jem.192.12.1719 2024-06-02T08:14:53+00:00 T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo Fassò, Marcella Anandasabapathy, Niroshana Crawford, Frances Kappler, John Fathman, C. Garrison Ridgway, William M. 2000 http://dx.doi.org/10.1084/jem.192.12.1719 https://rupress.org/jem/article-pdf/192/12/1719/1699640/990972.pdf en eng Rockefeller University Press The Journal of Experimental Medicine volume 192, issue 12, page 1719-1730 ISSN 0022-1007 1540-9538 journal-article 2000 crrockefelleruni https://doi.org/10.1084/jem.192.12.1719 2024-05-07T14:15:47Z We recently described a novel way to isolate populations of antigen-reactive CD4+ T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS® sorting by CD4high expression. Phenotypic, FACS®, functional, antibody inhibition, and major histocompatibility complex–peptide tetramer analyses, as well as T cell receptor Vβ sequence analyses, of the antigen-specific CD4high T cell populations demonstrated that a diverse sperm whale myoglobin 110–121–reactive CD4+ T cell repertoire was activated at the beginning (day 3 after immunization) of the immune response. Within 6 d of immunization, lower affinity clones were lost from the responding population, leaving an expanded population of oligoclonal, intermediate affinity (and residual high affinity) T cells. This T cell subset persisted for at least 4 wk after immunization and dominated the secondary immune response. These data provide evidence that CD4+ T cell repertoire selection occurs early in the immune response in vivo and suggest that persistence and expansion of a population of oligoclonal, intermediate affinity T cells is involved in CD4+ T cell memory. Article in Journal/Newspaper Sperm whale Rockefeller University Press Journal of Experimental Medicine 192 12 1719 1730 |
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Open Polar |
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Rockefeller University Press |
op_collection_id |
crrockefelleruni |
language |
English |
description |
We recently described a novel way to isolate populations of antigen-reactive CD4+ T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS® sorting by CD4high expression. Phenotypic, FACS®, functional, antibody inhibition, and major histocompatibility complex–peptide tetramer analyses, as well as T cell receptor Vβ sequence analyses, of the antigen-specific CD4high T cell populations demonstrated that a diverse sperm whale myoglobin 110–121–reactive CD4+ T cell repertoire was activated at the beginning (day 3 after immunization) of the immune response. Within 6 d of immunization, lower affinity clones were lost from the responding population, leaving an expanded population of oligoclonal, intermediate affinity (and residual high affinity) T cells. This T cell subset persisted for at least 4 wk after immunization and dominated the secondary immune response. These data provide evidence that CD4+ T cell repertoire selection occurs early in the immune response in vivo and suggest that persistence and expansion of a population of oligoclonal, intermediate affinity T cells is involved in CD4+ T cell memory. |
format |
Article in Journal/Newspaper |
author |
Fassò, Marcella Anandasabapathy, Niroshana Crawford, Frances Kappler, John Fathman, C. Garrison Ridgway, William M. |
spellingShingle |
Fassò, Marcella Anandasabapathy, Niroshana Crawford, Frances Kappler, John Fathman, C. Garrison Ridgway, William M. T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
author_facet |
Fassò, Marcella Anandasabapathy, Niroshana Crawford, Frances Kappler, John Fathman, C. Garrison Ridgway, William M. |
author_sort |
Fassò, Marcella |
title |
T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
title_short |
T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
title_full |
T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
title_fullStr |
T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
title_full_unstemmed |
T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr Vβ Diversity during the Initiation of a Cd4+ T Cell Response in Vivo |
title_sort |
t cell receptor (tcr)-mediated repertoire selection and loss of tcr vβ diversity during the initiation of a cd4+ t cell response in vivo |
publisher |
Rockefeller University Press |
publishDate |
2000 |
url |
http://dx.doi.org/10.1084/jem.192.12.1719 https://rupress.org/jem/article-pdf/192/12/1719/1699640/990972.pdf |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
The Journal of Experimental Medicine volume 192, issue 12, page 1719-1730 ISSN 0022-1007 1540-9538 |
op_doi |
https://doi.org/10.1084/jem.192.12.1719 |
container_title |
Journal of Experimental Medicine |
container_volume |
192 |
container_issue |
12 |
container_start_page |
1719 |
op_container_end_page |
1730 |
_version_ |
1800738893293682688 |