Influences of antigen processing on the expression of the T cell repertoire. Evidence for MHC-specific hindering structures on the products of processing.
Two lines of evidence in the current study indicate that antigen processing is a major factor, in addition to MHC binding and T cell repertoire, that determines Ir gene responsiveness and epitope immunodominance. First, immunization with synthetic peptides of myoglobin sequences revealed new reactiv...
Published in: | Journal of Experimental Medicine |
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Rockefeller University Press
1988
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Online Access: | http://dx.doi.org/10.1084/jem.168.1.357 https://rupress.org/jem/article-pdf/168/1/357/1668893/357.pdf |
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crrockefelleruni:10.1084/jem.168.1.357 2024-06-02T08:14:52+00:00 Influences of antigen processing on the expression of the T cell repertoire. Evidence for MHC-specific hindering structures on the products of processing. Brett, S J Cease, K B Berzofsky, J A 1988 http://dx.doi.org/10.1084/jem.168.1.357 https://rupress.org/jem/article-pdf/168/1/357/1668893/357.pdf en eng Rockefeller University Press The Journal of experimental medicine volume 168, issue 1, page 357-373 ISSN 0022-1007 1540-9538 journal-article 1988 crrockefelleruni https://doi.org/10.1084/jem.168.1.357 2024-05-07T14:15:19Z Two lines of evidence in the current study indicate that antigen processing is a major factor, in addition to MHC binding and T cell repertoire, that determines Ir gene responsiveness and epitope immunodominance. First, immunization with synthetic peptides of myoglobin sequences revealed new reactivities that had not appeared after priming with native myoglobin. For example, B10.S mice (H-2S) immune to equine myoglobin predominantly responded to peptide 102-118, whereas there was little, if any, response to this peptide in B10.BR (H-2k) mice immunized with native equine myoglobin. However, after immunization with the 102-118 peptide, both strains responded to the peptide. After in vitro restimulation, B10.BR T cells responded as well as B10.S T cells. Similarly, some individual 102-118-specific T cell clones from mice of both haplotypes showed similar dose responses and fine specificity patterns. Thus, low responsiveness to this site is due neither to a hole in the repertoire nor to a failure to bind to the appropriate MHC molecule. An alternative explanation was suggested by the observation that, whereas B10.S T cells from peptide 102-118-immune mice responded almost as well to whole myoglobin as to the peptide, the B10.BR T cells from peptide immune mice, while responding well to peptide, were poorly stimulated by whole myoglobin. Thus, the product of natural processing of equine myoglobin probably has hindering structures in the regions flanking the core epitope 102-118 that interfere with presentation by I-Ak but not I-AS. The second line of evidence that processing of native myoglobin may influence the apparent specificity of the T cell response was obtained using the I-Ad-restricted sperm whale myoglobin 102-118-specific clone 9.27. This clone discriminated readily between whole sperm whale myoglobin and equine myoglobin, but it did not distinguish between peptides corresponding to 102-118 of the sperm whale and equine sequences. This distinction between equine peptide and native equine myoglobin could be ... Article in Journal/Newspaper Sperm whale Rockefeller University Press Journal of Experimental Medicine 168 1 357 373 |
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Open Polar |
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Rockefeller University Press |
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crrockefelleruni |
language |
English |
description |
Two lines of evidence in the current study indicate that antigen processing is a major factor, in addition to MHC binding and T cell repertoire, that determines Ir gene responsiveness and epitope immunodominance. First, immunization with synthetic peptides of myoglobin sequences revealed new reactivities that had not appeared after priming with native myoglobin. For example, B10.S mice (H-2S) immune to equine myoglobin predominantly responded to peptide 102-118, whereas there was little, if any, response to this peptide in B10.BR (H-2k) mice immunized with native equine myoglobin. However, after immunization with the 102-118 peptide, both strains responded to the peptide. After in vitro restimulation, B10.BR T cells responded as well as B10.S T cells. Similarly, some individual 102-118-specific T cell clones from mice of both haplotypes showed similar dose responses and fine specificity patterns. Thus, low responsiveness to this site is due neither to a hole in the repertoire nor to a failure to bind to the appropriate MHC molecule. An alternative explanation was suggested by the observation that, whereas B10.S T cells from peptide 102-118-immune mice responded almost as well to whole myoglobin as to the peptide, the B10.BR T cells from peptide immune mice, while responding well to peptide, were poorly stimulated by whole myoglobin. Thus, the product of natural processing of equine myoglobin probably has hindering structures in the regions flanking the core epitope 102-118 that interfere with presentation by I-Ak but not I-AS. The second line of evidence that processing of native myoglobin may influence the apparent specificity of the T cell response was obtained using the I-Ad-restricted sperm whale myoglobin 102-118-specific clone 9.27. This clone discriminated readily between whole sperm whale myoglobin and equine myoglobin, but it did not distinguish between peptides corresponding to 102-118 of the sperm whale and equine sequences. This distinction between equine peptide and native equine myoglobin could be ... |
format |
Article in Journal/Newspaper |
author |
Brett, S J Cease, K B Berzofsky, J A |
spellingShingle |
Brett, S J Cease, K B Berzofsky, J A Influences of antigen processing on the expression of the T cell repertoire. Evidence for MHC-specific hindering structures on the products of processing. |
author_facet |
Brett, S J Cease, K B Berzofsky, J A |
author_sort |
Brett, S J |
title |
Influences of antigen processing on the expression of the T cell repertoire. Evidence for MHC-specific hindering structures on the products of processing. |
title_short |
Influences of antigen processing on the expression of the T cell repertoire. Evidence for MHC-specific hindering structures on the products of processing. |
title_full |
Influences of antigen processing on the expression of the T cell repertoire. Evidence for MHC-specific hindering structures on the products of processing. |
title_fullStr |
Influences of antigen processing on the expression of the T cell repertoire. Evidence for MHC-specific hindering structures on the products of processing. |
title_full_unstemmed |
Influences of antigen processing on the expression of the T cell repertoire. Evidence for MHC-specific hindering structures on the products of processing. |
title_sort |
influences of antigen processing on the expression of the t cell repertoire. evidence for mhc-specific hindering structures on the products of processing. |
publisher |
Rockefeller University Press |
publishDate |
1988 |
url |
http://dx.doi.org/10.1084/jem.168.1.357 https://rupress.org/jem/article-pdf/168/1/357/1668893/357.pdf |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
The Journal of experimental medicine volume 168, issue 1, page 357-373 ISSN 0022-1007 1540-9538 |
op_doi |
https://doi.org/10.1084/jem.168.1.357 |
container_title |
Journal of Experimental Medicine |
container_volume |
168 |
container_issue |
1 |
container_start_page |
357 |
op_container_end_page |
373 |
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1800738872677629952 |