Limiting dilution comparison of the repertoires of high and low responder MHC-restricted T cells.
To approach the mechanism that determines Ir gene-controlled high or low responsiveness to whole proteins, such as sperm whale myoglobin (SWMb), we compared the repertoires of high and low responder haplotype-restricted T cells for different myoglobin epitopes by limiting dilution frequency analysis...
Published in: | Journal of Experimental Medicine |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Rockefeller University Press
1988
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1084/jem.167.3.1100 https://rupress.org/jem/article-pdf/167/3/1100/1668359/1100.pdf |
id |
crrockefelleruni:10.1084/jem.167.3.1100 |
---|---|
record_format |
openpolar |
spelling |
crrockefelleruni:10.1084/jem.167.3.1100 2024-06-02T08:14:54+00:00 Limiting dilution comparison of the repertoires of high and low responder MHC-restricted T cells. Kojima, M Cease, K B Buckenmeyer, G K Berzofsky, J A 1988 http://dx.doi.org/10.1084/jem.167.3.1100 https://rupress.org/jem/article-pdf/167/3/1100/1668359/1100.pdf en eng Rockefeller University Press The Journal of experimental medicine volume 167, issue 3, page 1100-1113 ISSN 0022-1007 1540-9538 journal-article 1988 crrockefelleruni https://doi.org/10.1084/jem.167.3.1100 2024-05-07T14:15:19Z To approach the mechanism that determines Ir gene-controlled high or low responsiveness to whole proteins, such as sperm whale myoglobin (SWMb), we compared the repertoires of high and low responder haplotype-restricted T cells for different myoglobin epitopes by limiting dilution frequency analysis. Poisson analysis was performed using long-term limiting dilution cell lines of (B10.BR [low] X B10.D2[high])F1 T cells maintained on high or low responder APCs. The cell lines were tested with SWMb peptides and fragments for T cell repertoire fine specificities and Ia restrictions. The frequency of SWMb-specific F1 T cells responsive on B10.BR (H-2k) APCs was 2.5-3.6-fold lower than on B10.D2 (H-2d) APCs. Strikingly, all of the H-2k-restricted T cells used I-Ek as a restriction element, whereas both I-Ad- and I-Ed-restricted T cells were found among the H-2d-restricted lines. The I-Ad-restricted T cells were dominant, and the majority was specific for the synthetic peptide 102-118. T cells specific for peptide 132-146, dominant in association with I-Ed, were less frequent. However, no detectable H-2k-restricted T cells were specific for either of these peptides, but instead they were specific for fragment 1-55 or peptide 59-80. Fragment 1-55 also stimulated a similar number of H-2d-restricted T cells. Therefore, the low response of F1 T cells on H-2k-presenting cells may be due to the failure to see myoglobin plus I-Ak, in particular the immunodominant site around Glu 109, in contrast to the dominant response of high responder mice (both H-2d and H-2s) focused on the I-A molecule and the site around residue Glu 109. The I-E- low responder B10 strain also failed to respond to peptide 102-118, supporting the idea that the low responder status results from a limited repertoire lacking response to 102-118 plus I-A. In those strains that respond to the immunodominant site 102-118, the frequency of T cells in the repertoire specific for this site was always considerably greater than that for other sites. These results ... Article in Journal/Newspaper Sperm whale Rockefeller University Press Journal of Experimental Medicine 167 3 1100 1113 |
institution |
Open Polar |
collection |
Rockefeller University Press |
op_collection_id |
crrockefelleruni |
language |
English |
description |
To approach the mechanism that determines Ir gene-controlled high or low responsiveness to whole proteins, such as sperm whale myoglobin (SWMb), we compared the repertoires of high and low responder haplotype-restricted T cells for different myoglobin epitopes by limiting dilution frequency analysis. Poisson analysis was performed using long-term limiting dilution cell lines of (B10.BR [low] X B10.D2[high])F1 T cells maintained on high or low responder APCs. The cell lines were tested with SWMb peptides and fragments for T cell repertoire fine specificities and Ia restrictions. The frequency of SWMb-specific F1 T cells responsive on B10.BR (H-2k) APCs was 2.5-3.6-fold lower than on B10.D2 (H-2d) APCs. Strikingly, all of the H-2k-restricted T cells used I-Ek as a restriction element, whereas both I-Ad- and I-Ed-restricted T cells were found among the H-2d-restricted lines. The I-Ad-restricted T cells were dominant, and the majority was specific for the synthetic peptide 102-118. T cells specific for peptide 132-146, dominant in association with I-Ed, were less frequent. However, no detectable H-2k-restricted T cells were specific for either of these peptides, but instead they were specific for fragment 1-55 or peptide 59-80. Fragment 1-55 also stimulated a similar number of H-2d-restricted T cells. Therefore, the low response of F1 T cells on H-2k-presenting cells may be due to the failure to see myoglobin plus I-Ak, in particular the immunodominant site around Glu 109, in contrast to the dominant response of high responder mice (both H-2d and H-2s) focused on the I-A molecule and the site around residue Glu 109. The I-E- low responder B10 strain also failed to respond to peptide 102-118, supporting the idea that the low responder status results from a limited repertoire lacking response to 102-118 plus I-A. In those strains that respond to the immunodominant site 102-118, the frequency of T cells in the repertoire specific for this site was always considerably greater than that for other sites. These results ... |
format |
Article in Journal/Newspaper |
author |
Kojima, M Cease, K B Buckenmeyer, G K Berzofsky, J A |
spellingShingle |
Kojima, M Cease, K B Buckenmeyer, G K Berzofsky, J A Limiting dilution comparison of the repertoires of high and low responder MHC-restricted T cells. |
author_facet |
Kojima, M Cease, K B Buckenmeyer, G K Berzofsky, J A |
author_sort |
Kojima, M |
title |
Limiting dilution comparison of the repertoires of high and low responder MHC-restricted T cells. |
title_short |
Limiting dilution comparison of the repertoires of high and low responder MHC-restricted T cells. |
title_full |
Limiting dilution comparison of the repertoires of high and low responder MHC-restricted T cells. |
title_fullStr |
Limiting dilution comparison of the repertoires of high and low responder MHC-restricted T cells. |
title_full_unstemmed |
Limiting dilution comparison of the repertoires of high and low responder MHC-restricted T cells. |
title_sort |
limiting dilution comparison of the repertoires of high and low responder mhc-restricted t cells. |
publisher |
Rockefeller University Press |
publishDate |
1988 |
url |
http://dx.doi.org/10.1084/jem.167.3.1100 https://rupress.org/jem/article-pdf/167/3/1100/1668359/1100.pdf |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
The Journal of experimental medicine volume 167, issue 3, page 1100-1113 ISSN 0022-1007 1540-9538 |
op_doi |
https://doi.org/10.1084/jem.167.3.1100 |
container_title |
Journal of Experimental Medicine |
container_volume |
167 |
container_issue |
3 |
container_start_page |
1100 |
op_container_end_page |
1113 |
_version_ |
1800738909377789952 |