A new miniature hydrostatic pressure chamber for microscopy. Strain-free optical glass windows facilitate phase-contrast and polarized-light microscopy of living cells. Optional fixture permits simultaneous control of pressure and temperature.
This paper describes the development of a miniature, temperature-controlled, stainless steel pressure chamber which uses strain-free optical glass for windows. It is directly adaptable to standard phase-contrast and polarized-light microscopes and requires a minimum amount of equipment to generate a...
Published in: | Journal of Cell Biology |
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Language: | English |
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Rockefeller University Press
1975
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Online Access: | http://dx.doi.org/10.1083/jcb.65.3.587 https://rupress.org/jcb/article-pdf/65/3/587/1630170/587.pdf |
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crrockefelleruni:10.1083/jcb.65.3.587 2024-06-23T07:52:23+00:00 A new miniature hydrostatic pressure chamber for microscopy. Strain-free optical glass windows facilitate phase-contrast and polarized-light microscopy of living cells. Optional fixture permits simultaneous control of pressure and temperature. Salmon, E D Ellis, G W 1975 http://dx.doi.org/10.1083/jcb.65.3.587 https://rupress.org/jcb/article-pdf/65/3/587/1630170/587.pdf en eng Rockefeller University Press The Journal of cell biology volume 65, issue 3, page 587-602 ISSN 0021-9525 1540-8140 journal-article 1975 crrockefelleruni https://doi.org/10.1083/jcb.65.3.587 2024-06-04T06:22:23Z This paper describes the development of a miniature, temperature-controlled, stainless steel pressure chamber which uses strain-free optical glass for windows. It is directly adaptable to standard phase-contrast and polarized-light microscopes and requires a minimum amount of equipment to generate and measure pressure. Birefringence retardation (BR) og 0.1 nm up to 3,000 psi, 0.4 nm up to 5,000 psi and 1.0 nm up to 10,000 psi can be detected over a 0.75-mm central field with two strain-free Leitz 20 times UM objectives, one used as a condenser. In phase-contrast studies a Nikon DML 40 times phase objective and Zeiss model IS long working-distance phase condenser were used, with little deterioration of image quality or contrast at pressures as high as 12,000 psi. The actual design process required a synthesis of various criteria which may be categorized under four main areas of consideration: (a) specimen physiology; (b) constraints imposed by available optical equipment and standard microscope systems; (c) mechanical strength and methods for generating pressure; and (d) optical requirements of the chamber windows. Procedures for using the chambers, as well as methods for shifting and controlling the temperature within the chamber, are included. Article in Journal/Newspaper DML Rockefeller University Press Psi ENVELOPE(-63.000,-63.000,-64.300,-64.300) Journal of Cell Biology 65 3 587 602 |
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Open Polar |
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Rockefeller University Press |
op_collection_id |
crrockefelleruni |
language |
English |
description |
This paper describes the development of a miniature, temperature-controlled, stainless steel pressure chamber which uses strain-free optical glass for windows. It is directly adaptable to standard phase-contrast and polarized-light microscopes and requires a minimum amount of equipment to generate and measure pressure. Birefringence retardation (BR) og 0.1 nm up to 3,000 psi, 0.4 nm up to 5,000 psi and 1.0 nm up to 10,000 psi can be detected over a 0.75-mm central field with two strain-free Leitz 20 times UM objectives, one used as a condenser. In phase-contrast studies a Nikon DML 40 times phase objective and Zeiss model IS long working-distance phase condenser were used, with little deterioration of image quality or contrast at pressures as high as 12,000 psi. The actual design process required a synthesis of various criteria which may be categorized under four main areas of consideration: (a) specimen physiology; (b) constraints imposed by available optical equipment and standard microscope systems; (c) mechanical strength and methods for generating pressure; and (d) optical requirements of the chamber windows. Procedures for using the chambers, as well as methods for shifting and controlling the temperature within the chamber, are included. |
format |
Article in Journal/Newspaper |
author |
Salmon, E D Ellis, G W |
spellingShingle |
Salmon, E D Ellis, G W A new miniature hydrostatic pressure chamber for microscopy. Strain-free optical glass windows facilitate phase-contrast and polarized-light microscopy of living cells. Optional fixture permits simultaneous control of pressure and temperature. |
author_facet |
Salmon, E D Ellis, G W |
author_sort |
Salmon, E D |
title |
A new miniature hydrostatic pressure chamber for microscopy. Strain-free optical glass windows facilitate phase-contrast and polarized-light microscopy of living cells. Optional fixture permits simultaneous control of pressure and temperature. |
title_short |
A new miniature hydrostatic pressure chamber for microscopy. Strain-free optical glass windows facilitate phase-contrast and polarized-light microscopy of living cells. Optional fixture permits simultaneous control of pressure and temperature. |
title_full |
A new miniature hydrostatic pressure chamber for microscopy. Strain-free optical glass windows facilitate phase-contrast and polarized-light microscopy of living cells. Optional fixture permits simultaneous control of pressure and temperature. |
title_fullStr |
A new miniature hydrostatic pressure chamber for microscopy. Strain-free optical glass windows facilitate phase-contrast and polarized-light microscopy of living cells. Optional fixture permits simultaneous control of pressure and temperature. |
title_full_unstemmed |
A new miniature hydrostatic pressure chamber for microscopy. Strain-free optical glass windows facilitate phase-contrast and polarized-light microscopy of living cells. Optional fixture permits simultaneous control of pressure and temperature. |
title_sort |
new miniature hydrostatic pressure chamber for microscopy. strain-free optical glass windows facilitate phase-contrast and polarized-light microscopy of living cells. optional fixture permits simultaneous control of pressure and temperature. |
publisher |
Rockefeller University Press |
publishDate |
1975 |
url |
http://dx.doi.org/10.1083/jcb.65.3.587 https://rupress.org/jcb/article-pdf/65/3/587/1630170/587.pdf |
long_lat |
ENVELOPE(-63.000,-63.000,-64.300,-64.300) |
geographic |
Psi |
geographic_facet |
Psi |
genre |
DML |
genre_facet |
DML |
op_source |
The Journal of cell biology volume 65, issue 3, page 587-602 ISSN 0021-9525 1540-8140 |
op_doi |
https://doi.org/10.1083/jcb.65.3.587 |
container_title |
Journal of Cell Biology |
container_volume |
65 |
container_issue |
3 |
container_start_page |
587 |
op_container_end_page |
602 |
_version_ |
1802643674017824768 |