CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation
Inflammation is a key factor in the development of atherosclerotic coronary artery disease. It is promoted through the inflammasome, a molecular machine that produces IL (interleukin)-1β in response to cholesterol crystal accumulation in macrophages. The CARD8 (caspase recruitment domain 8) protein...
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crportlandpress:10.1042/cs20120572 2024-09-15T18:26:12+00:00 CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation Paramel, Geena Varghese Folkersen, Lasse Strawbridge, Rona J. Elmabsout, Ali Ateia Särndahl, Eva Lundman, Pia Jansson, Jan-Håkan Hansson, Göran K. Sirsjö, Allan Fransén, Karin 2013 http://dx.doi.org/10.1042/cs20120572 https://portlandpress.com/clinsci/article-pdf/125/8/401/443608/cs1250401.pdf en eng Portland Press Ltd. Clinical Science volume 125, issue 8, page 401-407 ISSN 0143-5221 1470-8736 journal-article 2013 crportlandpress https://doi.org/10.1042/cs20120572 2024-09-05T05:01:09Z Inflammation is a key factor in the development of atherosclerotic coronary artery disease. It is promoted through the inflammasome, a molecular machine that produces IL (interleukin)-1β in response to cholesterol crystal accumulation in macrophages. The CARD8 (caspase recruitment domain 8) protein modulates this process by suppressing caspase 1 and the transcription factor NF-κB (nuclear factor κB). The expression of CARD8 mRNA was examined in atherosclerotic vascular tissue and the impact on MI (myocardial infarction) of a polymorphism in the CARD8 gene determined. CARD8 mRNA was analysed by microarray of human atherosclerotic tissue and compared with transplant donor arterial tissue. Microarray analysis was performed for proximal genes associated with the rs2043211 locus in plaque. The CARD8 rs2043211 polymorphism was analysed by genotyping of two Swedish MI cohorts, FIA (First Myocardial Infarction in Northern Sweden) and SCARF (Stockholm Coronary Atherosclerosis Risk Factor). The CRP (C-reactive protein) level was measured in both cohorts, but the levels of the pro-inflammatory cytokines IL-1β, IL-18, TNF (tumour necrosis factor) and MCP-1 (monocyte chemoattractant protein) were measured in sera available from the SCARF cohort. CARD8 mRNA was highly expressed in atherosclerotic plaques compared with the expression in transplant donor vessel (P<0.00001). The minor allele was associated with lower expression of CARD8 in the plaques, suggesting that CARD8 may promote inflammation. Carriers of the minor allele of the rs2043211 polymorphism also displayed lower circulating CRP and lower levels of the pro-atherosclerotic chemokine MCP-1. However, no significant association could be detected between this polymorphism and MI in the two cohorts. Genetic alterations in the CARD8 gene therefore seem to be of limited importance for the development of MI. Article in Journal/Newspaper Northern Sweden Portland Press Clinical Science 125 8 401 407 |
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Portland Press |
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English |
description |
Inflammation is a key factor in the development of atherosclerotic coronary artery disease. It is promoted through the inflammasome, a molecular machine that produces IL (interleukin)-1β in response to cholesterol crystal accumulation in macrophages. The CARD8 (caspase recruitment domain 8) protein modulates this process by suppressing caspase 1 and the transcription factor NF-κB (nuclear factor κB). The expression of CARD8 mRNA was examined in atherosclerotic vascular tissue and the impact on MI (myocardial infarction) of a polymorphism in the CARD8 gene determined. CARD8 mRNA was analysed by microarray of human atherosclerotic tissue and compared with transplant donor arterial tissue. Microarray analysis was performed for proximal genes associated with the rs2043211 locus in plaque. The CARD8 rs2043211 polymorphism was analysed by genotyping of two Swedish MI cohorts, FIA (First Myocardial Infarction in Northern Sweden) and SCARF (Stockholm Coronary Atherosclerosis Risk Factor). The CRP (C-reactive protein) level was measured in both cohorts, but the levels of the pro-inflammatory cytokines IL-1β, IL-18, TNF (tumour necrosis factor) and MCP-1 (monocyte chemoattractant protein) were measured in sera available from the SCARF cohort. CARD8 mRNA was highly expressed in atherosclerotic plaques compared with the expression in transplant donor vessel (P<0.00001). The minor allele was associated with lower expression of CARD8 in the plaques, suggesting that CARD8 may promote inflammation. Carriers of the minor allele of the rs2043211 polymorphism also displayed lower circulating CRP and lower levels of the pro-atherosclerotic chemokine MCP-1. However, no significant association could be detected between this polymorphism and MI in the two cohorts. Genetic alterations in the CARD8 gene therefore seem to be of limited importance for the development of MI. |
format |
Article in Journal/Newspaper |
author |
Paramel, Geena Varghese Folkersen, Lasse Strawbridge, Rona J. Elmabsout, Ali Ateia Särndahl, Eva Lundman, Pia Jansson, Jan-Håkan Hansson, Göran K. Sirsjö, Allan Fransén, Karin |
spellingShingle |
Paramel, Geena Varghese Folkersen, Lasse Strawbridge, Rona J. Elmabsout, Ali Ateia Särndahl, Eva Lundman, Pia Jansson, Jan-Håkan Hansson, Göran K. Sirsjö, Allan Fransén, Karin CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation |
author_facet |
Paramel, Geena Varghese Folkersen, Lasse Strawbridge, Rona J. Elmabsout, Ali Ateia Särndahl, Eva Lundman, Pia Jansson, Jan-Håkan Hansson, Göran K. Sirsjö, Allan Fransén, Karin |
author_sort |
Paramel, Geena Varghese |
title |
CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation |
title_short |
CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation |
title_full |
CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation |
title_fullStr |
CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation |
title_full_unstemmed |
CARD8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation |
title_sort |
card8 gene encoding a protein of innate immunity is expressed in human atherosclerosis and associated with markers of inflammation |
publisher |
Portland Press Ltd. |
publishDate |
2013 |
url |
http://dx.doi.org/10.1042/cs20120572 https://portlandpress.com/clinsci/article-pdf/125/8/401/443608/cs1250401.pdf |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Clinical Science volume 125, issue 8, page 401-407 ISSN 0143-5221 1470-8736 |
op_doi |
https://doi.org/10.1042/cs20120572 |
container_title |
Clinical Science |
container_volume |
125 |
container_issue |
8 |
container_start_page |
401 |
op_container_end_page |
407 |
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1810466649707380736 |