Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels
Reverse transcriptase-mediated PCR has been used to isolate two distinct metallothionein (MT) cDNA species from RNA extracted from icefish liver, namely MT-I and MT-II. Northern blot analysis with these cDNA species revealed that significant endogenous levels of MT mRNA were present in liver tissues...
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Portland Press Ltd.
1998
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crportlandpress:10.1042/bj3320475 2024-09-09T19:09:39+00:00 Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels CARGINALE, Vincenzo SCUDIERO, Rosaria CAPASSO, Clemente CAPASSO, Antonio KILLE, Peter PRISCO, Guido di PARISI, Elio 1998 http://dx.doi.org/10.1042/bj3320475 https://portlandpress.com/biochemj/article-pdf/332/2/475/728690/bj3320475.pdf en eng Portland Press Ltd. Biochemical Journal volume 332, issue 2, page 475-481 ISSN 0264-6021 1470-8728 journal-article 1998 crportlandpress https://doi.org/10.1042/bj3320475 2024-07-18T04:20:45Z Reverse transcriptase-mediated PCR has been used to isolate two distinct metallothionein (MT) cDNA species from RNA extracted from icefish liver, namely MT-I and MT-II. Northern blot analysis with these cDNA species revealed that significant endogenous levels of MT mRNA were present in liver tissues of normal animals despite the fact that no MT protein could be found accumulating in the same tissue. However, multiple injections of CdCl2 induced high levels of both MT mRNA and MT protein. Sequence analysis of the cDNA species that were present after cadmium injection revealed the presence of both isoforms. Quantification of the MT-I and MT-II transcripts from normal and heavy-metal-treated fish showed an alteration in the ratio of the MT isoform transcripts. Endogenous transcripts consisted mostly of MT-II, whereas the MT-I transcript was preferentially accumulated only in response to the cadmium salt. The protein encoded by each cDNA isoform was isolated from the heavy-metal-treated fish and the availability of the specific MT mRNA for translation was demonstrated by translation in vitro. These results show that: (1) there is a discrepancy between the significant endogenous levels of MT mRNA and the absence of MT protein; (2) the accumulation of MT in icefish liver can be triggered by heavy metals; (3) genes encoding distinct MT isoforms are differentially regulated by heavy metals. Article in Journal/Newspaper Antarc* Antarctic Icefish Portland Press Antarctic The Antarctic Biochemical Journal 332 2 475 481 |
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Portland Press |
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English |
description |
Reverse transcriptase-mediated PCR has been used to isolate two distinct metallothionein (MT) cDNA species from RNA extracted from icefish liver, namely MT-I and MT-II. Northern blot analysis with these cDNA species revealed that significant endogenous levels of MT mRNA were present in liver tissues of normal animals despite the fact that no MT protein could be found accumulating in the same tissue. However, multiple injections of CdCl2 induced high levels of both MT mRNA and MT protein. Sequence analysis of the cDNA species that were present after cadmium injection revealed the presence of both isoforms. Quantification of the MT-I and MT-II transcripts from normal and heavy-metal-treated fish showed an alteration in the ratio of the MT isoform transcripts. Endogenous transcripts consisted mostly of MT-II, whereas the MT-I transcript was preferentially accumulated only in response to the cadmium salt. The protein encoded by each cDNA isoform was isolated from the heavy-metal-treated fish and the availability of the specific MT mRNA for translation was demonstrated by translation in vitro. These results show that: (1) there is a discrepancy between the significant endogenous levels of MT mRNA and the absence of MT protein; (2) the accumulation of MT in icefish liver can be triggered by heavy metals; (3) genes encoding distinct MT isoforms are differentially regulated by heavy metals. |
format |
Article in Journal/Newspaper |
author |
CARGINALE, Vincenzo SCUDIERO, Rosaria CAPASSO, Clemente CAPASSO, Antonio KILLE, Peter PRISCO, Guido di PARISI, Elio |
spellingShingle |
CARGINALE, Vincenzo SCUDIERO, Rosaria CAPASSO, Clemente CAPASSO, Antonio KILLE, Peter PRISCO, Guido di PARISI, Elio Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels |
author_facet |
CARGINALE, Vincenzo SCUDIERO, Rosaria CAPASSO, Clemente CAPASSO, Antonio KILLE, Peter PRISCO, Guido di PARISI, Elio |
author_sort |
CARGINALE, Vincenzo |
title |
Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels |
title_short |
Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels |
title_full |
Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels |
title_fullStr |
Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels |
title_full_unstemmed |
Cadmium-induced differential accumulation of metallothionein isoforms in the Antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mRNA levels |
title_sort |
cadmium-induced differential accumulation of metallothionein isoforms in the antarctic icefish, which exhibits no basal metallothionein protein but high endogenous mrna levels |
publisher |
Portland Press Ltd. |
publishDate |
1998 |
url |
http://dx.doi.org/10.1042/bj3320475 https://portlandpress.com/biochemj/article-pdf/332/2/475/728690/bj3320475.pdf |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Icefish |
genre_facet |
Antarc* Antarctic Icefish |
op_source |
Biochemical Journal volume 332, issue 2, page 475-481 ISSN 0264-6021 1470-8728 |
op_doi |
https://doi.org/10.1042/bj3320475 |
container_title |
Biochemical Journal |
container_volume |
332 |
container_issue |
2 |
container_start_page |
475 |
op_container_end_page |
481 |
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1809823895324196864 |