Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA
Icefish (family Channichthyidae, suborder Nothothenioidei) are a group of Antarctic fish that have evolved unique phenotypes in order to adapt to the environment in which they live. Besides the lack of haemoglobin and the drastic reduction in the number of erythrocyte-like cells, another striking fe...
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Portland Press Ltd.
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Online Access: | http://dx.doi.org/10.1042/bj3220207 https://portlandpress.com/biochemj/article-pdf/322/1/207/624087/bj3220207.pdf |
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crportlandpress:10.1042/bj3220207 2024-09-15T17:47:31+00:00 Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA SCUDIERO, Rosaria CARGINALE, Vincenzo RIGGIO, Marilisa CAPASSO, Clemente CAPASSO, Antonio KILLE, Peter PRISCO, Guido di PARISI, Elio 1997 http://dx.doi.org/10.1042/bj3220207 https://portlandpress.com/biochemj/article-pdf/322/1/207/624087/bj3220207.pdf en eng Portland Press Ltd. Biochemical Journal volume 322, issue 1, page 207-211 ISSN 0264-6021 1470-8728 journal-article 1997 crportlandpress https://doi.org/10.1042/bj3220207 2024-08-01T04:17:38Z Icefish (family Channichthyidae, suborder Nothothenioidei) are a group of Antarctic fish that have evolved unique phenotypes in order to adapt to the environment in which they live. Besides the lack of haemoglobin and the drastic reduction in the number of erythrocyte-like cells, another striking feature of the icefish is that their liver is devoid of metallothionein. These cysteine-rich heavy-metal-binding proteins are usually present in large amounts in a large variety of organisms, from bacteria to mammals. Despite the failure to detect appreciable levels of metallothionein in icefish liver, a cDNA encoding metallothionein was produced from total RNA by reverse transcriptase PCR. The icefish metallothionein showed high percentage identity with metallothionein from Trematomus bernacchii, a red-blooded Antarctic fish in which a normal content of hepatic metallothionein was found. Steady-state mRNA levels were assessed in fish liver by high-stringency hybridization of the metallothionein probe with total RNA. The results showed that icefish livers retain large amounts of untranslated metallothionein mRNA. The stability of the icefish transcript might be correlated with the lack of specific motifs in the untranslated 3ƀ ends of mRNA. Article in Journal/Newspaper Antarc* Antarctic Icefish Portland Press Biochemical Journal 322 1 207 211 |
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Open Polar |
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Portland Press |
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crportlandpress |
language |
English |
description |
Icefish (family Channichthyidae, suborder Nothothenioidei) are a group of Antarctic fish that have evolved unique phenotypes in order to adapt to the environment in which they live. Besides the lack of haemoglobin and the drastic reduction in the number of erythrocyte-like cells, another striking feature of the icefish is that their liver is devoid of metallothionein. These cysteine-rich heavy-metal-binding proteins are usually present in large amounts in a large variety of organisms, from bacteria to mammals. Despite the failure to detect appreciable levels of metallothionein in icefish liver, a cDNA encoding metallothionein was produced from total RNA by reverse transcriptase PCR. The icefish metallothionein showed high percentage identity with metallothionein from Trematomus bernacchii, a red-blooded Antarctic fish in which a normal content of hepatic metallothionein was found. Steady-state mRNA levels were assessed in fish liver by high-stringency hybridization of the metallothionein probe with total RNA. The results showed that icefish livers retain large amounts of untranslated metallothionein mRNA. The stability of the icefish transcript might be correlated with the lack of specific motifs in the untranslated 3ƀ ends of mRNA. |
format |
Article in Journal/Newspaper |
author |
SCUDIERO, Rosaria CARGINALE, Vincenzo RIGGIO, Marilisa CAPASSO, Clemente CAPASSO, Antonio KILLE, Peter PRISCO, Guido di PARISI, Elio |
spellingShingle |
SCUDIERO, Rosaria CARGINALE, Vincenzo RIGGIO, Marilisa CAPASSO, Clemente CAPASSO, Antonio KILLE, Peter PRISCO, Guido di PARISI, Elio Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA |
author_facet |
SCUDIERO, Rosaria CARGINALE, Vincenzo RIGGIO, Marilisa CAPASSO, Clemente CAPASSO, Antonio KILLE, Peter PRISCO, Guido di PARISI, Elio |
author_sort |
SCUDIERO, Rosaria |
title |
Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA |
title_short |
Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA |
title_full |
Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA |
title_fullStr |
Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA |
title_full_unstemmed |
Difference in hepatic metallothionein content in Antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mRNA |
title_sort |
difference in hepatic metallothionein content in antarctic red-blooded and haemoglobinless fish: undetectable metallothionein levels in haemoglobinless fish is accompanied by accumulation of untranslated metallothionein mrna |
publisher |
Portland Press Ltd. |
publishDate |
1997 |
url |
http://dx.doi.org/10.1042/bj3220207 https://portlandpress.com/biochemj/article-pdf/322/1/207/624087/bj3220207.pdf |
genre |
Antarc* Antarctic Icefish |
genre_facet |
Antarc* Antarctic Icefish |
op_source |
Biochemical Journal volume 322, issue 1, page 207-211 ISSN 0264-6021 1470-8728 |
op_doi |
https://doi.org/10.1042/bj3220207 |
container_title |
Biochemical Journal |
container_volume |
322 |
container_issue |
1 |
container_start_page |
207 |
op_container_end_page |
211 |
_version_ |
1810496924121300992 |