Substrate selectivities differ for hepatic mitochondrial and peroxisomal β-oxidation in an Antarctic fish, Notothenia gibberifrons
Hepatic mitochondrial and peroxisomal beta-oxidation were examined in an Antarctic marine teleost, Notothenia gibberifrons. Enzymic profiles and rates of beta-oxidation by intact organelles were determined by using a range of fatty acyl-CoA substrates to evaluate substrate preferences. Partitioning...
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Language: | English |
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Portland Press Ltd.
1993
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Online Access: | http://dx.doi.org/10.1042/bj2890427 https://portlandpress.com/biochemj/article-pdf/289/2/427/609021/bj2890427.pdf |
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crportlandpress:10.1042/bj2890427 2024-06-23T07:47:35+00:00 Substrate selectivities differ for hepatic mitochondrial and peroxisomal β-oxidation in an Antarctic fish, Notothenia gibberifrons Crockett, E L Sidell, B D 1993 http://dx.doi.org/10.1042/bj2890427 https://portlandpress.com/biochemj/article-pdf/289/2/427/609021/bj2890427.pdf en eng Portland Press Ltd. Biochemical Journal volume 289, issue 2, page 427-433 ISSN 0264-6021 1470-8728 journal-article 1993 crportlandpress https://doi.org/10.1042/bj2890427 2024-05-30T08:21:56Z Hepatic mitochondrial and peroxisomal beta-oxidation were examined in an Antarctic marine teleost, Notothenia gibberifrons. Enzymic profiles and rates of beta-oxidation by intact organelles were determined by using a range of fatty acyl-CoA substrates to evaluate substrate preferences. Partitioning of beta-oxidation between organelles was estimated. Substrate selectivities are broader for peroxisomal beta-oxidation than for mitochondrial beta-oxidation. Mitochondria show marked preference for the oxidation of a monounsaturated substrate, palmitoleoyl-CoA (C16:1), and two polyunsaturates, eicosapentaenoyl-CoA (C20:5) and docosahexaenoyl-CoA (C22:6). Carnitine palmitoyltransferase activities with palmitoleoyl-CoA (C16:1) are 2.4-fold higher than activities with palmitoyl-CoA (C16:0). Most polyunsaturated acyl-CoA esters measured appear to inhibit by over 40% the oxidation of palmitoyl-CoA by peroxisomes. Our findings suggest that the polyunsaturates, eicosapentaenoic acid (C20:5) and docosahexaenoic acid (C22:6), found in high concentrations in Antarctic fishes [Lund and Sidell (1992) Mar. Biol. 112, 377-382], are utilized as fuels to support aerobic energy metabolism. Metabolic capacities of rate-limiting enzymes and beta-oxidation rates by intact organelles indicate that up to 30% of hepatic beta-oxidation in N. gibberifrons can be initiated by the peroxisomal pathway. Article in Journal/Newspaper Antarc* Antarctic Portland Press Antarctic Biochemical Journal 289 2 427 433 |
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Portland Press |
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English |
description |
Hepatic mitochondrial and peroxisomal beta-oxidation were examined in an Antarctic marine teleost, Notothenia gibberifrons. Enzymic profiles and rates of beta-oxidation by intact organelles were determined by using a range of fatty acyl-CoA substrates to evaluate substrate preferences. Partitioning of beta-oxidation between organelles was estimated. Substrate selectivities are broader for peroxisomal beta-oxidation than for mitochondrial beta-oxidation. Mitochondria show marked preference for the oxidation of a monounsaturated substrate, palmitoleoyl-CoA (C16:1), and two polyunsaturates, eicosapentaenoyl-CoA (C20:5) and docosahexaenoyl-CoA (C22:6). Carnitine palmitoyltransferase activities with palmitoleoyl-CoA (C16:1) are 2.4-fold higher than activities with palmitoyl-CoA (C16:0). Most polyunsaturated acyl-CoA esters measured appear to inhibit by over 40% the oxidation of palmitoyl-CoA by peroxisomes. Our findings suggest that the polyunsaturates, eicosapentaenoic acid (C20:5) and docosahexaenoic acid (C22:6), found in high concentrations in Antarctic fishes [Lund and Sidell (1992) Mar. Biol. 112, 377-382], are utilized as fuels to support aerobic energy metabolism. Metabolic capacities of rate-limiting enzymes and beta-oxidation rates by intact organelles indicate that up to 30% of hepatic beta-oxidation in N. gibberifrons can be initiated by the peroxisomal pathway. |
format |
Article in Journal/Newspaper |
author |
Crockett, E L Sidell, B D |
spellingShingle |
Crockett, E L Sidell, B D Substrate selectivities differ for hepatic mitochondrial and peroxisomal β-oxidation in an Antarctic fish, Notothenia gibberifrons |
author_facet |
Crockett, E L Sidell, B D |
author_sort |
Crockett, E L |
title |
Substrate selectivities differ for hepatic mitochondrial and peroxisomal β-oxidation in an Antarctic fish, Notothenia gibberifrons |
title_short |
Substrate selectivities differ for hepatic mitochondrial and peroxisomal β-oxidation in an Antarctic fish, Notothenia gibberifrons |
title_full |
Substrate selectivities differ for hepatic mitochondrial and peroxisomal β-oxidation in an Antarctic fish, Notothenia gibberifrons |
title_fullStr |
Substrate selectivities differ for hepatic mitochondrial and peroxisomal β-oxidation in an Antarctic fish, Notothenia gibberifrons |
title_full_unstemmed |
Substrate selectivities differ for hepatic mitochondrial and peroxisomal β-oxidation in an Antarctic fish, Notothenia gibberifrons |
title_sort |
substrate selectivities differ for hepatic mitochondrial and peroxisomal β-oxidation in an antarctic fish, notothenia gibberifrons |
publisher |
Portland Press Ltd. |
publishDate |
1993 |
url |
http://dx.doi.org/10.1042/bj2890427 https://portlandpress.com/biochemj/article-pdf/289/2/427/609021/bj2890427.pdf |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Biochemical Journal volume 289, issue 2, page 427-433 ISSN 0264-6021 1470-8728 |
op_doi |
https://doi.org/10.1042/bj2890427 |
container_title |
Biochemical Journal |
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289 |
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2 |
container_start_page |
427 |
op_container_end_page |
433 |
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1802651719564263424 |