Genetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon
Epididymal glutathione peroxidase (GPX) has been suggested as a major factor in combating loss of fertility of spermatozoa due to lipid peroxidation. We report here the isolation and sequence of putative GPX cDNAs from rat (Rattus rattus) and cynomolgus-monkey (Macaca fascicularis) epididymis, which...
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Language: | English |
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Portland Press Ltd.
1992
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Online Access: | http://dx.doi.org/10.1042/bj2850863 https://portlandpress.com/biochemj/article-pdf/285/3/863/609760/bj2850863.pdf |
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crportlandpress:10.1042/bj2850863 2023-12-31T10:22:23+01:00 Genetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon Perry, A C F Jones, R Niang, L S P Jackson, R M Hall, L 1992 http://dx.doi.org/10.1042/bj2850863 https://portlandpress.com/biochemj/article-pdf/285/3/863/609760/bj2850863.pdf en eng Portland Press Ltd. Biochemical Journal volume 285, issue 3, page 863-870 ISSN 0264-6021 1470-8728 Cell Biology Molecular Biology Biochemistry journal-article 1992 crportlandpress https://doi.org/10.1042/bj2850863 2023-12-04T13:31:52Z Epididymal glutathione peroxidase (GPX) has been suggested as a major factor in combating loss of fertility of spermatozoa due to lipid peroxidation. We report here the isolation and sequence of putative GPX cDNAs from rat (Rattus rattus) and cynomolgus-monkey (Macaca fascicularis) epididymis, which exhibit marked sequence identity with known GPXs. In both species the cDNAs encode predicted preproteins containing 221 amino acid residues. Unlike other characterized GPX sequences, epididymal GPX mRNA does not contain a selenocysteine codon (UGA). However, sequence comparison and molecular-modelling studies suggest a high degree of structural conservation between epididymal and other GPXs. Transcripts corresponding to epididymal GPX are not detected in a variety of other tissues (liver, spleen, kidney and testis) and appear to be androgen-regulated in the epididymis. Article in Journal/Newspaper Rattus rattus Portland Press (via Crossref) Biochemical Journal 285 3 863 870 |
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Portland Press (via Crossref) |
op_collection_id |
crportlandpress |
language |
English |
topic |
Cell Biology Molecular Biology Biochemistry |
spellingShingle |
Cell Biology Molecular Biology Biochemistry Perry, A C F Jones, R Niang, L S P Jackson, R M Hall, L Genetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon |
topic_facet |
Cell Biology Molecular Biology Biochemistry |
description |
Epididymal glutathione peroxidase (GPX) has been suggested as a major factor in combating loss of fertility of spermatozoa due to lipid peroxidation. We report here the isolation and sequence of putative GPX cDNAs from rat (Rattus rattus) and cynomolgus-monkey (Macaca fascicularis) epididymis, which exhibit marked sequence identity with known GPXs. In both species the cDNAs encode predicted preproteins containing 221 amino acid residues. Unlike other characterized GPX sequences, epididymal GPX mRNA does not contain a selenocysteine codon (UGA). However, sequence comparison and molecular-modelling studies suggest a high degree of structural conservation between epididymal and other GPXs. Transcripts corresponding to epididymal GPX are not detected in a variety of other tissues (liver, spleen, kidney and testis) and appear to be androgen-regulated in the epididymis. |
format |
Article in Journal/Newspaper |
author |
Perry, A C F Jones, R Niang, L S P Jackson, R M Hall, L |
author_facet |
Perry, A C F Jones, R Niang, L S P Jackson, R M Hall, L |
author_sort |
Perry, A C F |
title |
Genetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon |
title_short |
Genetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon |
title_full |
Genetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon |
title_fullStr |
Genetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon |
title_full_unstemmed |
Genetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon |
title_sort |
genetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon |
publisher |
Portland Press Ltd. |
publishDate |
1992 |
url |
http://dx.doi.org/10.1042/bj2850863 https://portlandpress.com/biochemj/article-pdf/285/3/863/609760/bj2850863.pdf |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Biochemical Journal volume 285, issue 3, page 863-870 ISSN 0264-6021 1470-8728 |
op_doi |
https://doi.org/10.1042/bj2850863 |
container_title |
Biochemical Journal |
container_volume |
285 |
container_issue |
3 |
container_start_page |
863 |
op_container_end_page |
870 |
_version_ |
1786833489056759808 |