Electron transfer between horse ferritin and ferrihaemoproteins

• Published in: Biochemical Journal
• Main Authors: Kadir, F H A, al-Massad, F K, Fatemi, S J A, Singh, H K, Wilson, M T, Moore, G R
• Format: Article in Journal/Newspaper
• Language: English
• Published: Portland Press Ltd. 1991
• Subjects: Cell Biology; Molecular Biology; Biochemistry; Sperm whale
• Online Access: http://dx.doi.org/10.1042/bj2780817; https://portlandpress.com/biochemj/article-pdf/278/3/817/603913/bj2780817.pdf

Reactions of reduced horse spleen ferritin with horse and Saccharomyces cerevisiae ferricytochromes c, cow ferricytochrome b5, sperm-whale metmyoglobin and Pseudomonas aeruginosa ferricytochrome c-551 were investigated by u.v.-visible spectrophotometry. In all cases the reduced ferritin reduced the ferrihaemoproteins. The rate of reduction varied from less than 0.2 M-1.s-1 for metmyoglobin to 1.1 x 10(3) M-1.s-1 for horse ferricytochrome c (0.1 M-phosphate buffer, pH 7.4, at 25 degrees C). We conclude that the mechanism of ferrihaemoprotein reduction involves long-range electron transfer through the coat of ferritin and that such electron transfer is rapid enough to account for the rates of iron release observed by other workers in reductive release assays.