Functional consequences of haem orientational disorder in sperm-whale and yellow-fin-tuna myoglobins
Ligand-binding kinetics of native and reconstituted sperm-whale myoglobin were studied in relation to haem orientational disorder by rapid kinetic methods. In addition, native yellow-fin-tuna myoglobin with significant amount of haem disorder was also used. The O2 dissociation and association rates...
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Language: | English |
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Portland Press Ltd.
1987
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Online Access: | http://dx.doi.org/10.1042/bj2430205 https://portlandpress.com/biochemj/article-pdf/243/1/205/589912/bj2430205.pdf |
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crportlandpress:10.1042/bj2430205 2023-12-31T10:23:21+01:00 Functional consequences of haem orientational disorder in sperm-whale and yellow-fin-tuna myoglobins Aojula, H S Wilson, M T Morrison, I E G 1987 http://dx.doi.org/10.1042/bj2430205 https://portlandpress.com/biochemj/article-pdf/243/1/205/589912/bj2430205.pdf en eng Portland Press Ltd. Biochemical Journal volume 243, issue 1, page 205-210 ISSN 0264-6021 1470-8728 Cell Biology Molecular Biology Biochemistry journal-article 1987 crportlandpress https://doi.org/10.1042/bj2430205 2023-12-04T13:33:29Z Ligand-binding kinetics of native and reconstituted sperm-whale myoglobin were studied in relation to haem orientational disorder by rapid kinetic methods. In addition, native yellow-fin-tuna myoglobin with significant amount of haem disorder was also used. The O2 dissociation and association rates were found for the proteins with different degrees of haem disorder, and these results suggest that the isomers are characterized by almost identical kinetic parameters. Rates of CO recombination after photolysis were also identical for the two orientational isomers. The results clearly indicate that the rotation of the haem about the alpha-gamma meso axis has little or no effect on the ligand-binding properties of these myoglobins. Article in Journal/Newspaper Sperm whale Portland Press (via Crossref) Biochemical Journal 243 1 205 210 |
institution |
Open Polar |
collection |
Portland Press (via Crossref) |
op_collection_id |
crportlandpress |
language |
English |
topic |
Cell Biology Molecular Biology Biochemistry |
spellingShingle |
Cell Biology Molecular Biology Biochemistry Aojula, H S Wilson, M T Morrison, I E G Functional consequences of haem orientational disorder in sperm-whale and yellow-fin-tuna myoglobins |
topic_facet |
Cell Biology Molecular Biology Biochemistry |
description |
Ligand-binding kinetics of native and reconstituted sperm-whale myoglobin were studied in relation to haem orientational disorder by rapid kinetic methods. In addition, native yellow-fin-tuna myoglobin with significant amount of haem disorder was also used. The O2 dissociation and association rates were found for the proteins with different degrees of haem disorder, and these results suggest that the isomers are characterized by almost identical kinetic parameters. Rates of CO recombination after photolysis were also identical for the two orientational isomers. The results clearly indicate that the rotation of the haem about the alpha-gamma meso axis has little or no effect on the ligand-binding properties of these myoglobins. |
format |
Article in Journal/Newspaper |
author |
Aojula, H S Wilson, M T Morrison, I E G |
author_facet |
Aojula, H S Wilson, M T Morrison, I E G |
author_sort |
Aojula, H S |
title |
Functional consequences of haem orientational disorder in sperm-whale and yellow-fin-tuna myoglobins |
title_short |
Functional consequences of haem orientational disorder in sperm-whale and yellow-fin-tuna myoglobins |
title_full |
Functional consequences of haem orientational disorder in sperm-whale and yellow-fin-tuna myoglobins |
title_fullStr |
Functional consequences of haem orientational disorder in sperm-whale and yellow-fin-tuna myoglobins |
title_full_unstemmed |
Functional consequences of haem orientational disorder in sperm-whale and yellow-fin-tuna myoglobins |
title_sort |
functional consequences of haem orientational disorder in sperm-whale and yellow-fin-tuna myoglobins |
publisher |
Portland Press Ltd. |
publishDate |
1987 |
url |
http://dx.doi.org/10.1042/bj2430205 https://portlandpress.com/biochemj/article-pdf/243/1/205/589912/bj2430205.pdf |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
Biochemical Journal volume 243, issue 1, page 205-210 ISSN 0264-6021 1470-8728 |
op_doi |
https://doi.org/10.1042/bj2430205 |
container_title |
Biochemical Journal |
container_volume |
243 |
container_issue |
1 |
container_start_page |
205 |
op_container_end_page |
210 |
_version_ |
1786834923541233664 |