Studies on carbon monoxide binding by shark haemoglobin
The kinetics of the reactions of Pacific-porbeagle haemoglobin with CO were studied by flash-photolysis and stopped-flow methods, and the equilibrium binding curves for CO were measured in spectrophotometric titrations. Measurements were made in the pH range 6-8 and in the temperature range 0-40 deg...
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Format: | Article in Journal/Newspaper |
Language: | English |
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Portland Press Ltd.
1981
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Online Access: | http://dx.doi.org/10.1042/bj1970437 https://portlandpress.com/biochemj/article-pdf/197/2/437/576355/bj1970437.pdf |
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crportlandpress:10.1042/bj1970437 2024-06-09T07:49:10+00:00 Studies on carbon monoxide binding by shark haemoglobin Dickinson, F M Gibson, Q H 1981 http://dx.doi.org/10.1042/bj1970437 https://portlandpress.com/biochemj/article-pdf/197/2/437/576355/bj1970437.pdf en eng Portland Press Ltd. Biochemical Journal volume 197, issue 2, page 437-446 ISSN 0264-6021 journal-article 1981 crportlandpress https://doi.org/10.1042/bj1970437 2024-05-16T14:10:23Z The kinetics of the reactions of Pacific-porbeagle haemoglobin with CO were studied by flash-photolysis and stopped-flow methods, and the equilibrium binding curves for CO were measured in spectrophotometric titrations. Measurements were made in the pH range 6-8 and in the temperature range 0-40 degrees C. The results are discussed in terms of the allosteric model proposed by Monod, Wyman & Changeux [(1965) J. Mol. Biol. 12, 88-118]. Within this framework the results indicate that in the R-state the haem groups fall into two classes of different reactivity with different spectral characteristics, but that in the T-state the groups may be essentially equivalent. The physiological importance of the temperature-insensitivity of the equilibrium ligand-binding curves for porbeagle haemoglobin is discussed. Article in Journal/Newspaper Porbeagle Portland Press Pacific Wyman ENVELOPE(158.950,158.950,-83.900,-83.900) Biochemical Journal 197 2 437 446 |
institution |
Open Polar |
collection |
Portland Press |
op_collection_id |
crportlandpress |
language |
English |
description |
The kinetics of the reactions of Pacific-porbeagle haemoglobin with CO were studied by flash-photolysis and stopped-flow methods, and the equilibrium binding curves for CO were measured in spectrophotometric titrations. Measurements were made in the pH range 6-8 and in the temperature range 0-40 degrees C. The results are discussed in terms of the allosteric model proposed by Monod, Wyman & Changeux [(1965) J. Mol. Biol. 12, 88-118]. Within this framework the results indicate that in the R-state the haem groups fall into two classes of different reactivity with different spectral characteristics, but that in the T-state the groups may be essentially equivalent. The physiological importance of the temperature-insensitivity of the equilibrium ligand-binding curves for porbeagle haemoglobin is discussed. |
format |
Article in Journal/Newspaper |
author |
Dickinson, F M Gibson, Q H |
spellingShingle |
Dickinson, F M Gibson, Q H Studies on carbon monoxide binding by shark haemoglobin |
author_facet |
Dickinson, F M Gibson, Q H |
author_sort |
Dickinson, F M |
title |
Studies on carbon monoxide binding by shark haemoglobin |
title_short |
Studies on carbon monoxide binding by shark haemoglobin |
title_full |
Studies on carbon monoxide binding by shark haemoglobin |
title_fullStr |
Studies on carbon monoxide binding by shark haemoglobin |
title_full_unstemmed |
Studies on carbon monoxide binding by shark haemoglobin |
title_sort |
studies on carbon monoxide binding by shark haemoglobin |
publisher |
Portland Press Ltd. |
publishDate |
1981 |
url |
http://dx.doi.org/10.1042/bj1970437 https://portlandpress.com/biochemj/article-pdf/197/2/437/576355/bj1970437.pdf |
long_lat |
ENVELOPE(158.950,158.950,-83.900,-83.900) |
geographic |
Pacific Wyman |
geographic_facet |
Pacific Wyman |
genre |
Porbeagle |
genre_facet |
Porbeagle |
op_source |
Biochemical Journal volume 197, issue 2, page 437-446 ISSN 0264-6021 |
op_doi |
https://doi.org/10.1042/bj1970437 |
container_title |
Biochemical Journal |
container_volume |
197 |
container_issue |
2 |
container_start_page |
437 |
op_container_end_page |
446 |
_version_ |
1801381428633534464 |