Isolation, properties and amino acid sequence of a long-chain neurotoxin, Acanthophis antarcticus b, from the venom of an Australian snake (the common death adder, Acanthophis antarcticus)
The venom of an Australian elapid snake, the common death adder (Acanthophis antarcticus), was chromatographed on a CM-cellulose CM52 column. One of the neurotoxic components, Acanthophis antarcticus b (toxin Aa b) was isolated in about 9.4% (A280) yield. The complete amino acid sequence of toxin Aa...
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Portland Press Ltd.
1981
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crportlandpress:10.1042/bj1930899 2024-10-06T13:44:08+00:00 Isolation, properties and amino acid sequence of a long-chain neurotoxin, Acanthophis antarcticus b, from the venom of an Australian snake (the common death adder, Acanthophis antarcticus) Kim, H S Tamiya, N 1981 http://dx.doi.org/10.1042/bj1930899 https://portlandpress.com/biochemj/article-pdf/193/3/899/564458/bj1930899.pdf en eng Portland Press Ltd. Biochemical Journal volume 193, issue 3, page 899-906 ISSN 0264-6021 journal-article 1981 crportlandpress https://doi.org/10.1042/bj1930899 2024-09-12T05:00:32Z The venom of an Australian elapid snake, the common death adder (Acanthophis antarcticus), was chromatographed on a CM-cellulose CM52 column. One of the neurotoxic components, Acanthophis antarcticus b (toxin Aa b) was isolated in about 9.4% (A280) yield. The complete amino acid sequence of toxin Aa b was elucidated. Toxin Aa b is composed of 73 amino acid residues, with ten half-cystine residues, and has a formula weight of 8135. Toxin Aa b has no histidine or methionine residue in its sequence. The amino acid sequence of toxin Aa b is homologous with those of other neurotoxins with known sequences, although it is novel in having a valine residue at its N-terminus and an arginine residue at position-23, where a lysine residue is found in almost all the so-far-known neurotoxins. Irrespective of the latter replacement, the toxin Aa b is fully active, with an LD50 value (in mice) of 0.13 microgram/g body weight on intramuscular injection. Article in Journal/Newspaper Antarc* antarcticus Portland Press Biochemical Journal 193 3 899 906 |
institution |
Open Polar |
collection |
Portland Press |
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crportlandpress |
language |
English |
description |
The venom of an Australian elapid snake, the common death adder (Acanthophis antarcticus), was chromatographed on a CM-cellulose CM52 column. One of the neurotoxic components, Acanthophis antarcticus b (toxin Aa b) was isolated in about 9.4% (A280) yield. The complete amino acid sequence of toxin Aa b was elucidated. Toxin Aa b is composed of 73 amino acid residues, with ten half-cystine residues, and has a formula weight of 8135. Toxin Aa b has no histidine or methionine residue in its sequence. The amino acid sequence of toxin Aa b is homologous with those of other neurotoxins with known sequences, although it is novel in having a valine residue at its N-terminus and an arginine residue at position-23, where a lysine residue is found in almost all the so-far-known neurotoxins. Irrespective of the latter replacement, the toxin Aa b is fully active, with an LD50 value (in mice) of 0.13 microgram/g body weight on intramuscular injection. |
format |
Article in Journal/Newspaper |
author |
Kim, H S Tamiya, N |
spellingShingle |
Kim, H S Tamiya, N Isolation, properties and amino acid sequence of a long-chain neurotoxin, Acanthophis antarcticus b, from the venom of an Australian snake (the common death adder, Acanthophis antarcticus) |
author_facet |
Kim, H S Tamiya, N |
author_sort |
Kim, H S |
title |
Isolation, properties and amino acid sequence of a long-chain neurotoxin, Acanthophis antarcticus b, from the venom of an Australian snake (the common death adder, Acanthophis antarcticus) |
title_short |
Isolation, properties and amino acid sequence of a long-chain neurotoxin, Acanthophis antarcticus b, from the venom of an Australian snake (the common death adder, Acanthophis antarcticus) |
title_full |
Isolation, properties and amino acid sequence of a long-chain neurotoxin, Acanthophis antarcticus b, from the venom of an Australian snake (the common death adder, Acanthophis antarcticus) |
title_fullStr |
Isolation, properties and amino acid sequence of a long-chain neurotoxin, Acanthophis antarcticus b, from the venom of an Australian snake (the common death adder, Acanthophis antarcticus) |
title_full_unstemmed |
Isolation, properties and amino acid sequence of a long-chain neurotoxin, Acanthophis antarcticus b, from the venom of an Australian snake (the common death adder, Acanthophis antarcticus) |
title_sort |
isolation, properties and amino acid sequence of a long-chain neurotoxin, acanthophis antarcticus b, from the venom of an australian snake (the common death adder, acanthophis antarcticus) |
publisher |
Portland Press Ltd. |
publishDate |
1981 |
url |
http://dx.doi.org/10.1042/bj1930899 https://portlandpress.com/biochemj/article-pdf/193/3/899/564458/bj1930899.pdf |
genre |
Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_source |
Biochemical Journal volume 193, issue 3, page 899-906 ISSN 0264-6021 |
op_doi |
https://doi.org/10.1042/bj1930899 |
container_title |
Biochemical Journal |
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193 |
container_issue |
3 |
container_start_page |
899 |
op_container_end_page |
906 |
_version_ |
1812182536949858304 |