Nearest-neighbour analysis of myoglobin antigenic sites. Nearest-neighbour residues whose replacement can alter the environment of binding-site residue(s) and thus change their characteristics and binding capability
By using the known antigenic structure of sperm-whale myoglobin previously determined in this laboratory and the X-ray co-ordinates for the myoglobin molecule, we have calculated the nearest-atom distances between each of the residues of the antigenic sites and all the other amino acids of the myogl...
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Language: | English |
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Portland Press Ltd.
1980
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Online Access: | http://dx.doi.org/10.1042/bj1910673 https://portlandpress.com/biochemj/article-pdf/191/3/673/574752/bj1910673.pdf |
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crportlandpress:10.1042/bj1910673 2024-06-09T07:49:47+00:00 Nearest-neighbour analysis of myoglobin antigenic sites. Nearest-neighbour residues whose replacement can alter the environment of binding-site residue(s) and thus change their characteristics and binding capability Kazim, A L Atassi, M Z 1980 http://dx.doi.org/10.1042/bj1910673 https://portlandpress.com/biochemj/article-pdf/191/3/673/574752/bj1910673.pdf en eng Portland Press Ltd. Biochemical Journal volume 191, issue 3, page 673-680 ISSN 0264-6021 journal-article 1980 crportlandpress https://doi.org/10.1042/bj1910673 2024-05-16T14:10:29Z By using the known antigenic structure of sperm-whale myoglobin previously determined in this laboratory and the X-ray co-ordinates for the myoglobin molecule, we have calculated the nearest-atom distances between each of the residues of the antigenic sites and all the other amino acids of the myoglobin molecule. These calculations have enabled us to identify the nearest-neighbour residues to each of the residues in the five antigenic sites, and which thus describe the immediate molecular environment of the sites. The influences of chemical changes or replacements in these environmental residues on the binding capacity of an antigenic site, when considered together with replacements directly in the antigenic sites, are expected to account for the major effects and will be extremely useful in explaining the cross-reactions of myoglobins from various species. However, it is stressed that the analysis has limitations due to the qualitative estimates of the effects, the influences of substitutions of once-removed or even at more distant locations (especially when they are cumulative) and finally the influences of any conformational re-adjustments when these occur as a result of the replacement(s). Article in Journal/Newspaper Sperm whale Portland Press Biochemical Journal 191 3 673 680 |
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Open Polar |
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Portland Press |
op_collection_id |
crportlandpress |
language |
English |
description |
By using the known antigenic structure of sperm-whale myoglobin previously determined in this laboratory and the X-ray co-ordinates for the myoglobin molecule, we have calculated the nearest-atom distances between each of the residues of the antigenic sites and all the other amino acids of the myoglobin molecule. These calculations have enabled us to identify the nearest-neighbour residues to each of the residues in the five antigenic sites, and which thus describe the immediate molecular environment of the sites. The influences of chemical changes or replacements in these environmental residues on the binding capacity of an antigenic site, when considered together with replacements directly in the antigenic sites, are expected to account for the major effects and will be extremely useful in explaining the cross-reactions of myoglobins from various species. However, it is stressed that the analysis has limitations due to the qualitative estimates of the effects, the influences of substitutions of once-removed or even at more distant locations (especially when they are cumulative) and finally the influences of any conformational re-adjustments when these occur as a result of the replacement(s). |
format |
Article in Journal/Newspaper |
author |
Kazim, A L Atassi, M Z |
spellingShingle |
Kazim, A L Atassi, M Z Nearest-neighbour analysis of myoglobin antigenic sites. Nearest-neighbour residues whose replacement can alter the environment of binding-site residue(s) and thus change their characteristics and binding capability |
author_facet |
Kazim, A L Atassi, M Z |
author_sort |
Kazim, A L |
title |
Nearest-neighbour analysis of myoglobin antigenic sites. Nearest-neighbour residues whose replacement can alter the environment of binding-site residue(s) and thus change their characteristics and binding capability |
title_short |
Nearest-neighbour analysis of myoglobin antigenic sites. Nearest-neighbour residues whose replacement can alter the environment of binding-site residue(s) and thus change their characteristics and binding capability |
title_full |
Nearest-neighbour analysis of myoglobin antigenic sites. Nearest-neighbour residues whose replacement can alter the environment of binding-site residue(s) and thus change their characteristics and binding capability |
title_fullStr |
Nearest-neighbour analysis of myoglobin antigenic sites. Nearest-neighbour residues whose replacement can alter the environment of binding-site residue(s) and thus change their characteristics and binding capability |
title_full_unstemmed |
Nearest-neighbour analysis of myoglobin antigenic sites. Nearest-neighbour residues whose replacement can alter the environment of binding-site residue(s) and thus change their characteristics and binding capability |
title_sort |
nearest-neighbour analysis of myoglobin antigenic sites. nearest-neighbour residues whose replacement can alter the environment of binding-site residue(s) and thus change their characteristics and binding capability |
publisher |
Portland Press Ltd. |
publishDate |
1980 |
url |
http://dx.doi.org/10.1042/bj1910673 https://portlandpress.com/biochemj/article-pdf/191/3/673/574752/bj1910673.pdf |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
Biochemical Journal volume 191, issue 3, page 673-680 ISSN 0264-6021 |
op_doi |
https://doi.org/10.1042/bj1910673 |
container_title |
Biochemical Journal |
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191 |
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3 |
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673 |
op_container_end_page |
680 |
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1801382599072940032 |