The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate
1. (Na+ +K+)-dependent ATPase was partially purified from eel gills by a procedure in which the microsomal fraction of crude preparations of chloride cells was selectively extracted with sodium dodecyl sulphate. 2. The microsomal specific activity was increased 2-fold during optimal treatment with d...
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Portland Press Ltd.
1979
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Online Access: | http://dx.doi.org/10.1042/bj1790431 https://portlandpress.com/biochemj/article-pdf/179/2/431/571327/bj1790431.pdf |
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crportlandpress:10.1042/bj1790431 2024-06-23T07:45:33+00:00 The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate Bell, M V Sargent, J R 1979 http://dx.doi.org/10.1042/bj1790431 https://portlandpress.com/biochemj/article-pdf/179/2/431/571327/bj1790431.pdf en eng Portland Press Ltd. Biochemical Journal volume 179, issue 2, page 431-438 ISSN 0264-6021 journal-article 1979 crportlandpress https://doi.org/10.1042/bj1790431 2024-05-30T08:21:51Z 1. (Na+ +K+)-dependent ATPase was partially purified from eel gills by a procedure in which the microsomal fraction of crude preparations of chloride cells was selectively extracted with sodium dodecyl sulphate. 2. The microsomal specific activity was increased 2-fold during optimal treatment with detergent. 3. The final preparation (56% pure) had a specific activity of 341 mumol of ATP hydrolysed/h per mg of protein and a turnover number of 3560 min-1. The number of ouabain-binding sties equalled the number of sites phosphorylated by ATP. 4. Both sodium orthovanadate and ouabain inhibited the purified preparation more than the microsomal fraction, vanadate being more effective on an equimolar basis than ouabain. 5. Inhibition by orthovanadate was not enhanced at 28 mM-as compared with 1mM-MgCl2 and was not reversed by beta-adrenergic agonists (cf. Josephson & Cantley (1977) Biochemistry 16, 4572–4578). 6. Of various other metallic oxyanions tested only niobate proved an effective inhibitor of the enzyme although this anion was less effective than orthovanadate. 7. Orthovanadate partially inhibited phosphorylation of the enzyme by ATP in the presence of 28 mM-MgCl2. Article in Journal/Newspaper Anguilla anguilla Portland Press Biochemical Journal 179 2 431 438 |
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Portland Press |
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English |
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1. (Na+ +K+)-dependent ATPase was partially purified from eel gills by a procedure in which the microsomal fraction of crude preparations of chloride cells was selectively extracted with sodium dodecyl sulphate. 2. The microsomal specific activity was increased 2-fold during optimal treatment with detergent. 3. The final preparation (56% pure) had a specific activity of 341 mumol of ATP hydrolysed/h per mg of protein and a turnover number of 3560 min-1. The number of ouabain-binding sties equalled the number of sites phosphorylated by ATP. 4. Both sodium orthovanadate and ouabain inhibited the purified preparation more than the microsomal fraction, vanadate being more effective on an equimolar basis than ouabain. 5. Inhibition by orthovanadate was not enhanced at 28 mM-as compared with 1mM-MgCl2 and was not reversed by beta-adrenergic agonists (cf. Josephson & Cantley (1977) Biochemistry 16, 4572–4578). 6. Of various other metallic oxyanions tested only niobate proved an effective inhibitor of the enzyme although this anion was less effective than orthovanadate. 7. Orthovanadate partially inhibited phosphorylation of the enzyme by ATP in the presence of 28 mM-MgCl2. |
format |
Article in Journal/Newspaper |
author |
Bell, M V Sargent, J R |
spellingShingle |
Bell, M V Sargent, J R The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate |
author_facet |
Bell, M V Sargent, J R |
author_sort |
Bell, M V |
title |
The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate |
title_short |
The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate |
title_full |
The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate |
title_fullStr |
The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate |
title_full_unstemmed |
The partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of Anguilla anguilla and its inhibition by orthovanadate |
title_sort |
partial purification of sodium-plus-potassium ion-dependent adenosine triphosphatase from the gills of anguilla anguilla and its inhibition by orthovanadate |
publisher |
Portland Press Ltd. |
publishDate |
1979 |
url |
http://dx.doi.org/10.1042/bj1790431 https://portlandpress.com/biochemj/article-pdf/179/2/431/571327/bj1790431.pdf |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Biochemical Journal volume 179, issue 2, page 431-438 ISSN 0264-6021 |
op_doi |
https://doi.org/10.1042/bj1790431 |
container_title |
Biochemical Journal |
container_volume |
179 |
container_issue |
2 |
container_start_page |
431 |
op_container_end_page |
438 |
_version_ |
1802640817025712128 |