An investigation by dielectric methods of hydration in myoglobin solutions
The permittivities of three solutions of sperm-whale myoglobin of different concentrations were measured in the frequency range 300–1300MHz at 20°C by using a coaxial-line technique. These results were combined with those measured previously at frequencies below 10MHz. Two methods are described for...
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Language: | English |
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Portland Press Ltd.
1974
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Online Access: | http://dx.doi.org/10.1042/bj1390375 https://portlandpress.com/biochemj/article-pdf/139/2/375/562089/bj1390375.pdf |
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crportlandpress:10.1042/bj1390375 2024-06-09T07:49:47+00:00 An investigation by dielectric methods of hydration in myoglobin solutions Grant, Edward H. Mitton, Barbara G. R. South, Gerald P. Sheppard, Rodney J. 1974 http://dx.doi.org/10.1042/bj1390375 https://portlandpress.com/biochemj/article-pdf/139/2/375/562089/bj1390375.pdf en eng Portland Press Ltd. Biochemical Journal volume 139, issue 2, page 375-380 ISSN 0264-6021 journal-article 1974 crportlandpress https://doi.org/10.1042/bj1390375 2024-05-16T14:10:36Z The permittivities of three solutions of sperm-whale myoglobin of different concentrations were measured in the frequency range 300–1300MHz at 20°C by using a coaxial-line technique. These results were combined with those measured previously at frequencies below 10MHz. Two methods are described for calculating the extent of macromolecular hydration from the data. The more reliable method yields results of approx. 0.25g of H2O/g of protein, which is in satisfactory agreement with the theoretically calculated value. Agreement with the value found from the rotational motion of the molecule is not so close, which is probably caused by the different meanings that may be ascribed to the term hydration. Article in Journal/Newspaper Sperm whale Portland Press Biochemical Journal 139 2 375 380 |
institution |
Open Polar |
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Portland Press |
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crportlandpress |
language |
English |
description |
The permittivities of three solutions of sperm-whale myoglobin of different concentrations were measured in the frequency range 300–1300MHz at 20°C by using a coaxial-line technique. These results were combined with those measured previously at frequencies below 10MHz. Two methods are described for calculating the extent of macromolecular hydration from the data. The more reliable method yields results of approx. 0.25g of H2O/g of protein, which is in satisfactory agreement with the theoretically calculated value. Agreement with the value found from the rotational motion of the molecule is not so close, which is probably caused by the different meanings that may be ascribed to the term hydration. |
format |
Article in Journal/Newspaper |
author |
Grant, Edward H. Mitton, Barbara G. R. South, Gerald P. Sheppard, Rodney J. |
spellingShingle |
Grant, Edward H. Mitton, Barbara G. R. South, Gerald P. Sheppard, Rodney J. An investigation by dielectric methods of hydration in myoglobin solutions |
author_facet |
Grant, Edward H. Mitton, Barbara G. R. South, Gerald P. Sheppard, Rodney J. |
author_sort |
Grant, Edward H. |
title |
An investigation by dielectric methods of hydration in myoglobin solutions |
title_short |
An investigation by dielectric methods of hydration in myoglobin solutions |
title_full |
An investigation by dielectric methods of hydration in myoglobin solutions |
title_fullStr |
An investigation by dielectric methods of hydration in myoglobin solutions |
title_full_unstemmed |
An investigation by dielectric methods of hydration in myoglobin solutions |
title_sort |
investigation by dielectric methods of hydration in myoglobin solutions |
publisher |
Portland Press Ltd. |
publishDate |
1974 |
url |
http://dx.doi.org/10.1042/bj1390375 https://portlandpress.com/biochemj/article-pdf/139/2/375/562089/bj1390375.pdf |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
Biochemical Journal volume 139, issue 2, page 375-380 ISSN 0264-6021 |
op_doi |
https://doi.org/10.1042/bj1390375 |
container_title |
Biochemical Journal |
container_volume |
139 |
container_issue |
2 |
container_start_page |
375 |
op_container_end_page |
380 |
_version_ |
1801382593112834048 |