Eġġ proteins in cod serum. Natural occurrence and induction by injections of oestradiol 3-benzoate
1. Before the uptake of water that precedes spawning, eggs of cod (Gadus morhua L.) contained 30% dry matter, of which 80% was protein. Some 75% of this protein was soluble in 0.5m-sodium chloride. The major components in the extract were two similar lipoproteins, of molecular weight about 400000, c...
Published in: | Biochemical Journal |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Portland Press Ltd.
1971
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1042/bj1210847 https://portlandpress.com/biochemj/article-pdf/121/5/847/770271/bj1210847.pdf |
id |
crportlandpress:10.1042/bj1210847 |
---|---|
record_format |
openpolar |
spelling |
crportlandpress:10.1042/bj1210847 2024-06-09T07:46:05+00:00 Eġġ proteins in cod serum. Natural occurrence and induction by injections of oestradiol 3-benzoate Plack, P. A. Pritchard, D. J. Fraser, N. W. 1971 http://dx.doi.org/10.1042/bj1210847 https://portlandpress.com/biochemj/article-pdf/121/5/847/770271/bj1210847.pdf en eng Portland Press Ltd. Biochemical Journal volume 121, issue 5, page 847-856 ISSN 0306-3283 journal-article 1971 crportlandpress https://doi.org/10.1042/bj1210847 2024-05-16T14:10:24Z 1. Before the uptake of water that precedes spawning, eggs of cod (Gadus morhua L.) contained 30% dry matter, of which 80% was protein. Some 75% of this protein was soluble in 0.5m-sodium chloride. The major components in the extract were two similar lipoproteins, of molecular weight about 400000, containing 21% lipid, some two-thirds of which was phospholipid, and about 0.5% protein phosphorus. 2. These lipoproteins were identified by immunochemical methods in the serum of female cod with developing ovaries, but not in the serum of male or of immature female fish. 3. The concentrations of egg proteins in the serum of female cod were determined by a serial-dilution double-diffusion immunological method, and were shown to increase with development of the ovaries, reaching a value of about 32mg/ml when the weight of the ovaries was 10% of the weight of the fish. 4. Immature male and female cod were injected intramuscularly with a solution of oestradiol-17β 3-benzoate in oil and the concentration of egg proteins in their serum was measured by the immunodiffusion method. The serum contained no detectable egg proteins before injection of the fish, but 30μg of oestradiol benzoate/kg gave rise to detectable amounts of egg proteins in 10 days, and with 300μg or 1mg of oestradiol benzoate/kg the concentration of egg proteins rose to 32mg/ml. The values for male and female cod were similar and represented about one-half of the total serum protein. 5. With a dose of 1mg of oestradiol benzoate/kg, egg proteins were first detected in the serum 2 days after injection and the concentration increased up to 10 days. 6. Serum samples taken before and 10 days after an injection of 1mg of oestradiol benzoate/kg were fractionated by gel-filtration on Sephadex G-200. The difference curves obtained from fractionation curves after and before injection confirmed the values of the concentrations of egg proteins obtained from the immunodiffusion test and showed that the concentrations of the normal serum components fell by 20–50% of the ... Article in Journal/Newspaper Gadus morhua Portland Press Biochemical Journal 121 5 847 856 |
institution |
Open Polar |
collection |
Portland Press |
op_collection_id |
crportlandpress |
language |
English |
description |
1. Before the uptake of water that precedes spawning, eggs of cod (Gadus morhua L.) contained 30% dry matter, of which 80% was protein. Some 75% of this protein was soluble in 0.5m-sodium chloride. The major components in the extract were two similar lipoproteins, of molecular weight about 400000, containing 21% lipid, some two-thirds of which was phospholipid, and about 0.5% protein phosphorus. 2. These lipoproteins were identified by immunochemical methods in the serum of female cod with developing ovaries, but not in the serum of male or of immature female fish. 3. The concentrations of egg proteins in the serum of female cod were determined by a serial-dilution double-diffusion immunological method, and were shown to increase with development of the ovaries, reaching a value of about 32mg/ml when the weight of the ovaries was 10% of the weight of the fish. 4. Immature male and female cod were injected intramuscularly with a solution of oestradiol-17β 3-benzoate in oil and the concentration of egg proteins in their serum was measured by the immunodiffusion method. The serum contained no detectable egg proteins before injection of the fish, but 30μg of oestradiol benzoate/kg gave rise to detectable amounts of egg proteins in 10 days, and with 300μg or 1mg of oestradiol benzoate/kg the concentration of egg proteins rose to 32mg/ml. The values for male and female cod were similar and represented about one-half of the total serum protein. 5. With a dose of 1mg of oestradiol benzoate/kg, egg proteins were first detected in the serum 2 days after injection and the concentration increased up to 10 days. 6. Serum samples taken before and 10 days after an injection of 1mg of oestradiol benzoate/kg were fractionated by gel-filtration on Sephadex G-200. The difference curves obtained from fractionation curves after and before injection confirmed the values of the concentrations of egg proteins obtained from the immunodiffusion test and showed that the concentrations of the normal serum components fell by 20–50% of the ... |
format |
Article in Journal/Newspaper |
author |
Plack, P. A. Pritchard, D. J. Fraser, N. W. |
spellingShingle |
Plack, P. A. Pritchard, D. J. Fraser, N. W. Eġġ proteins in cod serum. Natural occurrence and induction by injections of oestradiol 3-benzoate |
author_facet |
Plack, P. A. Pritchard, D. J. Fraser, N. W. |
author_sort |
Plack, P. A. |
title |
Eġġ proteins in cod serum. Natural occurrence and induction by injections of oestradiol 3-benzoate |
title_short |
Eġġ proteins in cod serum. Natural occurrence and induction by injections of oestradiol 3-benzoate |
title_full |
Eġġ proteins in cod serum. Natural occurrence and induction by injections of oestradiol 3-benzoate |
title_fullStr |
Eġġ proteins in cod serum. Natural occurrence and induction by injections of oestradiol 3-benzoate |
title_full_unstemmed |
Eġġ proteins in cod serum. Natural occurrence and induction by injections of oestradiol 3-benzoate |
title_sort |
eġġ proteins in cod serum. natural occurrence and induction by injections of oestradiol 3-benzoate |
publisher |
Portland Press Ltd. |
publishDate |
1971 |
url |
http://dx.doi.org/10.1042/bj1210847 https://portlandpress.com/biochemj/article-pdf/121/5/847/770271/bj1210847.pdf |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Biochemical Journal volume 121, issue 5, page 847-856 ISSN 0306-3283 |
op_doi |
https://doi.org/10.1042/bj1210847 |
container_title |
Biochemical Journal |
container_volume |
121 |
container_issue |
5 |
container_start_page |
847 |
op_container_end_page |
856 |
_version_ |
1801375844193533952 |