Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers

• Published in: Biochemical Journal
• Main Authors: Boegman, R. J., Crumpton, M. J.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Portland Press Ltd. 1970
• Subjects: Sperm whale
• Online Access: http://dx.doi.org/10.1042/bj1200373; https://portlandpress.com/biochemj/article-pdf/120/2/373/769262/bj1200373.pdf

Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene–maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparations indicated that Sepharose–myoglobin was superior, as an immunoadsorbent, to the other conjugates. Elution of adsorbed antibodies with m-propionic acid, pH2.5, gave yields of precipitable antibody higher than those achieved with other dissociating solvents. Propionic acid eluted 60–100% of the antibody adsorbed by Sepharose–myoglobin, and 60–90% of the eluted protein was precipitable antibody.