Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification
Mytilus coruscus is an economically important marine bivalve mollusk found in the Yangtze River estuary, which experiences dramatic pH fluctuations due to seasonal freshwater input and suffer from shell fracture or injury in the natural environment. In this study, we used intact-shell and damaged-sh...
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Online Access: | http://dx.doi.org/10.1371/journal.pone.0293565 https://dx.plos.org/10.1371/journal.pone.0293565 |
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crplos:10.1371/journal.pone.0293565 2024-05-19T07:46:37+00:00 Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification Fan, Xiaojun Wang, Ying Tang, Changsheng Zhang, Xiaolin He, Jianyu Buttino, Isabella Yan, Xiaojun Liao, Zhi Mukherjee, Amitava National Natural Science Foundation of China National Natural Science Foundation of China National Natural Science Foundation of China Zhejiang Provincial Natural Science Foundation Zhejiang Provincial Natural Science Foundation 2023 http://dx.doi.org/10.1371/journal.pone.0293565 https://dx.plos.org/10.1371/journal.pone.0293565 en eng Public Library of Science (PLoS) http://creativecommons.org/licenses/by/4.0/ PLOS ONE volume 18, issue 10, page e0293565 ISSN 1932-6203 journal-article 2023 crplos https://doi.org/10.1371/journal.pone.0293565 2024-05-01T06:59:35Z Mytilus coruscus is an economically important marine bivalve mollusk found in the Yangtze River estuary, which experiences dramatic pH fluctuations due to seasonal freshwater input and suffer from shell fracture or injury in the natural environment. In this study, we used intact-shell and damaged-shell M . coruscus and performed metabolomic analysis, free amino acids analysis, calcium-positive staining, and intracellular calcium level tests in the mantle to investigate whether the mantle-specific metabolites can be induced by acute sea-water acidification and understand how the mantle responds to acute acidification during the shell repair process. We observed that both shell damage and acute acidification induced alterations in phospholipids, amino acids, nucleotides, organic acids, benzenoids, and their analogs and derivatives. Glycylproline, spicamycin, and 2-aminoheptanoic acid (2-AHA) are explicitly induced by shell damage. Betaine, aspartate, and oxidized glutathione are specifically induced by acute acidification. Our results show different metabolic patterns in the mussel mantle in response to different stressors, which can help elucidate the shell repair process under ocean acidification. furthermore, metabolic processes related to energy supply, cell function, signal transduction, and amino acid synthesis are disturbed by shell damage and/or acute acidification, indicating that both shell damage and acute acidification increased energy consumption, and disturb phospholipid synthesis, osmotic regulation, and redox balance. Free amino acid analysis and enzymatic activity assays partially confirmed our findings, highlighting the adaptation of M . coruscus to dramatic pH fluctuations in the Yangtze River estuary. Article in Journal/Newspaper Ocean acidification PLOS PLOS ONE 18 10 e0293565 |
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English |
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Mytilus coruscus is an economically important marine bivalve mollusk found in the Yangtze River estuary, which experiences dramatic pH fluctuations due to seasonal freshwater input and suffer from shell fracture or injury in the natural environment. In this study, we used intact-shell and damaged-shell M . coruscus and performed metabolomic analysis, free amino acids analysis, calcium-positive staining, and intracellular calcium level tests in the mantle to investigate whether the mantle-specific metabolites can be induced by acute sea-water acidification and understand how the mantle responds to acute acidification during the shell repair process. We observed that both shell damage and acute acidification induced alterations in phospholipids, amino acids, nucleotides, organic acids, benzenoids, and their analogs and derivatives. Glycylproline, spicamycin, and 2-aminoheptanoic acid (2-AHA) are explicitly induced by shell damage. Betaine, aspartate, and oxidized glutathione are specifically induced by acute acidification. Our results show different metabolic patterns in the mussel mantle in response to different stressors, which can help elucidate the shell repair process under ocean acidification. furthermore, metabolic processes related to energy supply, cell function, signal transduction, and amino acid synthesis are disturbed by shell damage and/or acute acidification, indicating that both shell damage and acute acidification increased energy consumption, and disturb phospholipid synthesis, osmotic regulation, and redox balance. Free amino acid analysis and enzymatic activity assays partially confirmed our findings, highlighting the adaptation of M . coruscus to dramatic pH fluctuations in the Yangtze River estuary. |
author2 |
Mukherjee, Amitava National Natural Science Foundation of China National Natural Science Foundation of China National Natural Science Foundation of China Zhejiang Provincial Natural Science Foundation Zhejiang Provincial Natural Science Foundation |
format |
Article in Journal/Newspaper |
author |
Fan, Xiaojun Wang, Ying Tang, Changsheng Zhang, Xiaolin He, Jianyu Buttino, Isabella Yan, Xiaojun Liao, Zhi |
spellingShingle |
Fan, Xiaojun Wang, Ying Tang, Changsheng Zhang, Xiaolin He, Jianyu Buttino, Isabella Yan, Xiaojun Liao, Zhi Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification |
author_facet |
Fan, Xiaojun Wang, Ying Tang, Changsheng Zhang, Xiaolin He, Jianyu Buttino, Isabella Yan, Xiaojun Liao, Zhi |
author_sort |
Fan, Xiaojun |
title |
Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification |
title_short |
Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification |
title_full |
Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification |
title_fullStr |
Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification |
title_full_unstemmed |
Metabolic profiling of Mytilus coruscus mantle in response of shell repairing under acute acidification |
title_sort |
metabolic profiling of mytilus coruscus mantle in response of shell repairing under acute acidification |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1371/journal.pone.0293565 https://dx.plos.org/10.1371/journal.pone.0293565 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
PLOS ONE volume 18, issue 10, page e0293565 ISSN 1932-6203 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1371/journal.pone.0293565 |
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PLOS ONE |
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18 |
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10 |
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e0293565 |
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