A simple method to isolate fatty acids and fatty alcohols from wax esters in a wax-ester rich marine oil
Calanus finmarchicus is one of the most important zooplankton species in the North Atlantic. The zooplankton is currently being harvested and industrially processed to a marine oil product for human consumption as a marine nutraceutical containing long-chain omega-3 polyunsaturated fatty acids. This...
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crplos:10.1371/journal.pone.0285751 2024-09-09T19:34:58+00:00 A simple method to isolate fatty acids and fatty alcohols from wax esters in a wax-ester rich marine oil Schots, Pauke Carlijn Edvinsen, Guro Kristine Olsen, Ragnar Ludvig Ramanan, Rishiram Publication Fund of UiT The Arctic University of Norway 2023 http://dx.doi.org/10.1371/journal.pone.0285751 https://dx.plos.org/10.1371/journal.pone.0285751 en eng Public Library of Science (PLoS) http://creativecommons.org/licenses/by/4.0/ PLOS ONE volume 18, issue 5, page e0285751 ISSN 1932-6203 journal-article 2023 crplos https://doi.org/10.1371/journal.pone.0285751 2024-06-18T04:09:05Z Calanus finmarchicus is one of the most important zooplankton species in the North Atlantic. The zooplankton is currently being harvested and industrially processed to a marine oil product for human consumption as a marine nutraceutical containing long-chain omega-3 polyunsaturated fatty acids. This oil is very rich in wax esters, a lipid class where fatty acids are esterified to long chain fatty alcohols. In this paper we describe a simple method to 1) isolate the wax esters from the other lipid classes present in the oil, 2) hydrolyze the wax esters, and 3) separate the fatty acids from the fatty alcohol, all by means of solid phase extraction. Starting with an average of 322 mg Calanus oil, we obtained 75 mg fatty alcohols and 63 mg fatty acids. Contrary to previously described techniques, our method neither oxidize the fatty alcohols to fatty acids, nor are the fatty acids methylated, allowing the native, unesterified fatty acids and fatty alcohols to be used for further studies, such as in cell culture experiments to study the metabolic effects of these specific lipid fractions rather than the intact oil or wax esters. Article in Journal/Newspaper Calanus finmarchicus North Atlantic PLOS PLOS ONE 18 5 e0285751 |
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language |
English |
description |
Calanus finmarchicus is one of the most important zooplankton species in the North Atlantic. The zooplankton is currently being harvested and industrially processed to a marine oil product for human consumption as a marine nutraceutical containing long-chain omega-3 polyunsaturated fatty acids. This oil is very rich in wax esters, a lipid class where fatty acids are esterified to long chain fatty alcohols. In this paper we describe a simple method to 1) isolate the wax esters from the other lipid classes present in the oil, 2) hydrolyze the wax esters, and 3) separate the fatty acids from the fatty alcohol, all by means of solid phase extraction. Starting with an average of 322 mg Calanus oil, we obtained 75 mg fatty alcohols and 63 mg fatty acids. Contrary to previously described techniques, our method neither oxidize the fatty alcohols to fatty acids, nor are the fatty acids methylated, allowing the native, unesterified fatty acids and fatty alcohols to be used for further studies, such as in cell culture experiments to study the metabolic effects of these specific lipid fractions rather than the intact oil or wax esters. |
author2 |
Ramanan, Rishiram Publication Fund of UiT The Arctic University of Norway |
format |
Article in Journal/Newspaper |
author |
Schots, Pauke Carlijn Edvinsen, Guro Kristine Olsen, Ragnar Ludvig |
spellingShingle |
Schots, Pauke Carlijn Edvinsen, Guro Kristine Olsen, Ragnar Ludvig A simple method to isolate fatty acids and fatty alcohols from wax esters in a wax-ester rich marine oil |
author_facet |
Schots, Pauke Carlijn Edvinsen, Guro Kristine Olsen, Ragnar Ludvig |
author_sort |
Schots, Pauke Carlijn |
title |
A simple method to isolate fatty acids and fatty alcohols from wax esters in a wax-ester rich marine oil |
title_short |
A simple method to isolate fatty acids and fatty alcohols from wax esters in a wax-ester rich marine oil |
title_full |
A simple method to isolate fatty acids and fatty alcohols from wax esters in a wax-ester rich marine oil |
title_fullStr |
A simple method to isolate fatty acids and fatty alcohols from wax esters in a wax-ester rich marine oil |
title_full_unstemmed |
A simple method to isolate fatty acids and fatty alcohols from wax esters in a wax-ester rich marine oil |
title_sort |
simple method to isolate fatty acids and fatty alcohols from wax esters in a wax-ester rich marine oil |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1371/journal.pone.0285751 https://dx.plos.org/10.1371/journal.pone.0285751 |
genre |
Calanus finmarchicus North Atlantic |
genre_facet |
Calanus finmarchicus North Atlantic |
op_source |
PLOS ONE volume 18, issue 5, page e0285751 ISSN 1932-6203 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1371/journal.pone.0285751 |
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PLOS ONE |
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18 |
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5 |
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e0285751 |
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1809904394901127168 |