Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)
Serratia marcescens is a species that belongs to the family of Yersiniaceae . This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3 R ) of the species Serratia marc...
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crplos:10.1371/journal.pone.0259673 2024-09-15T18:10:22+00:00 Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) Friedrich, Ines Bodenberger, Bernhard Neubauer, Hannes Hertel, Robert Daniel, Rolf Kuo, Chih-Horng 2021 http://dx.doi.org/10.1371/journal.pone.0259673 https://dx.plos.org/10.1371/journal.pone.0259673 en eng Public Library of Science (PLoS) http://creativecommons.org/licenses/by/4.0/ PLOS ONE volume 16, issue 11, page e0259673 ISSN 1932-6203 journal-article 2021 crplos https://doi.org/10.1371/journal.pone.0259673 2024-08-06T04:10:53Z Serratia marcescens is a species that belongs to the family of Yersiniaceae . This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3 R ) of the species Serratia marcescens . The strain was characterized genomically, morphologically, and physiologically. In addition, the potential of the isolate to act as a host strain to assess the diversity of Serratia associated phages in environmental samples was explored. Average nucleotide identity analysis revealed that LVF3 R belongs to the species Serratia marcescens . In silico analysis and ProphageSeq data resulted in the identification of one prophage, which is capable of viral particle formation. Electron microscopy showed cells of a rod-shaped, flagellated morphotype. The cells revealed a length and width of 1–1.6 μm and 0.8 μm, respectively. LVF3 R showed optimal growth at 30 C and in the presence of up to 2% (w/v) NaCl. It exhibited resistances to ampicillin, erythromycin, oxacillin, oxytetracycline, rifampicin, tetracycline, and vancomycin. Genome data indicate that strain S . marcescens LVF3 R is a potential PGPR strain. It harbors genes coding for indole acetic acid ( IAA) biosynthesis, siderophore production, plant polymer degradation enzymes, acetoin synthesis, flagellar proteins, type IV secretion system, chemotaxis, phosphorous solubilization, and biofilm formation. Article in Journal/Newspaper Groenlandia PLOS PLOS ONE 16 11 e0259673 |
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Serratia marcescens is a species that belongs to the family of Yersiniaceae . This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3 R ) of the species Serratia marcescens . The strain was characterized genomically, morphologically, and physiologically. In addition, the potential of the isolate to act as a host strain to assess the diversity of Serratia associated phages in environmental samples was explored. Average nucleotide identity analysis revealed that LVF3 R belongs to the species Serratia marcescens . In silico analysis and ProphageSeq data resulted in the identification of one prophage, which is capable of viral particle formation. Electron microscopy showed cells of a rod-shaped, flagellated morphotype. The cells revealed a length and width of 1–1.6 μm and 0.8 μm, respectively. LVF3 R showed optimal growth at 30 C and in the presence of up to 2% (w/v) NaCl. It exhibited resistances to ampicillin, erythromycin, oxacillin, oxytetracycline, rifampicin, tetracycline, and vancomycin. Genome data indicate that strain S . marcescens LVF3 R is a potential PGPR strain. It harbors genes coding for indole acetic acid ( IAA) biosynthesis, siderophore production, plant polymer degradation enzymes, acetoin synthesis, flagellar proteins, type IV secretion system, chemotaxis, phosphorous solubilization, and biofilm formation. |
author2 |
Kuo, Chih-Horng |
format |
Article in Journal/Newspaper |
author |
Friedrich, Ines Bodenberger, Bernhard Neubauer, Hannes Hertel, Robert Daniel, Rolf |
spellingShingle |
Friedrich, Ines Bodenberger, Bernhard Neubauer, Hannes Hertel, Robert Daniel, Rolf Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
author_facet |
Friedrich, Ines Bodenberger, Bernhard Neubauer, Hannes Hertel, Robert Daniel, Rolf |
author_sort |
Friedrich, Ines |
title |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_short |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_full |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_fullStr |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_full_unstemmed |
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) |
title_sort |
down in the pond: isolation and characterization of a new serratia marcescens strain (lvf3) from the surface water near frog’s lettuce (groenlandia densa) |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1371/journal.pone.0259673 https://dx.plos.org/10.1371/journal.pone.0259673 |
genre |
Groenlandia |
genre_facet |
Groenlandia |
op_source |
PLOS ONE volume 16, issue 11, page e0259673 ISSN 1932-6203 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1371/journal.pone.0259673 |
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PLOS ONE |
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16 |
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11 |
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