Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)

Serratia marcescens is a species that belongs to the family of Yersiniaceae . This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3 R ) of the species Serratia marc...

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Published in:PLOS ONE
Main Authors: Friedrich, Ines, Bodenberger, Bernhard, Neubauer, Hannes, Hertel, Robert, Daniel, Rolf
Other Authors: Kuo, Chih-Horng
Format: Article in Journal/Newspaper
Language:English
Published: Public Library of Science (PLoS) 2021
Subjects:
Online Access:http://dx.doi.org/10.1371/journal.pone.0259673
https://dx.plos.org/10.1371/journal.pone.0259673
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spelling crplos:10.1371/journal.pone.0259673 2024-09-15T18:10:22+00:00 Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa) Friedrich, Ines Bodenberger, Bernhard Neubauer, Hannes Hertel, Robert Daniel, Rolf Kuo, Chih-Horng 2021 http://dx.doi.org/10.1371/journal.pone.0259673 https://dx.plos.org/10.1371/journal.pone.0259673 en eng Public Library of Science (PLoS) http://creativecommons.org/licenses/by/4.0/ PLOS ONE volume 16, issue 11, page e0259673 ISSN 1932-6203 journal-article 2021 crplos https://doi.org/10.1371/journal.pone.0259673 2024-08-06T04:10:53Z Serratia marcescens is a species that belongs to the family of Yersiniaceae . This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3 R ) of the species Serratia marcescens . The strain was characterized genomically, morphologically, and physiologically. In addition, the potential of the isolate to act as a host strain to assess the diversity of Serratia associated phages in environmental samples was explored. Average nucleotide identity analysis revealed that LVF3 R belongs to the species Serratia marcescens . In silico analysis and ProphageSeq data resulted in the identification of one prophage, which is capable of viral particle formation. Electron microscopy showed cells of a rod-shaped, flagellated morphotype. The cells revealed a length and width of 1–1.6 μm and 0.8 μm, respectively. LVF3 R showed optimal growth at 30 C and in the presence of up to 2% (w/v) NaCl. It exhibited resistances to ampicillin, erythromycin, oxacillin, oxytetracycline, rifampicin, tetracycline, and vancomycin. Genome data indicate that strain S . marcescens LVF3 R is a potential PGPR strain. It harbors genes coding for indole acetic acid ( IAA) biosynthesis, siderophore production, plant polymer degradation enzymes, acetoin synthesis, flagellar proteins, type IV secretion system, chemotaxis, phosphorous solubilization, and biofilm formation. Article in Journal/Newspaper Groenlandia PLOS PLOS ONE 16 11 e0259673
institution Open Polar
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op_collection_id crplos
language English
description Serratia marcescens is a species that belongs to the family of Yersiniaceae . This family comprises taxa representing opportunistic human- and phytopathogens but also plant growth-promoting rhizobacteria (PGPR). This study describes a novel Gram-negative strain (LVF3 R ) of the species Serratia marcescens . The strain was characterized genomically, morphologically, and physiologically. In addition, the potential of the isolate to act as a host strain to assess the diversity of Serratia associated phages in environmental samples was explored. Average nucleotide identity analysis revealed that LVF3 R belongs to the species Serratia marcescens . In silico analysis and ProphageSeq data resulted in the identification of one prophage, which is capable of viral particle formation. Electron microscopy showed cells of a rod-shaped, flagellated morphotype. The cells revealed a length and width of 1–1.6 μm and 0.8 μm, respectively. LVF3 R showed optimal growth at 30 C and in the presence of up to 2% (w/v) NaCl. It exhibited resistances to ampicillin, erythromycin, oxacillin, oxytetracycline, rifampicin, tetracycline, and vancomycin. Genome data indicate that strain S . marcescens LVF3 R is a potential PGPR strain. It harbors genes coding for indole acetic acid ( IAA) biosynthesis, siderophore production, plant polymer degradation enzymes, acetoin synthesis, flagellar proteins, type IV secretion system, chemotaxis, phosphorous solubilization, and biofilm formation.
author2 Kuo, Chih-Horng
format Article in Journal/Newspaper
author Friedrich, Ines
Bodenberger, Bernhard
Neubauer, Hannes
Hertel, Robert
Daniel, Rolf
spellingShingle Friedrich, Ines
Bodenberger, Bernhard
Neubauer, Hannes
Hertel, Robert
Daniel, Rolf
Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)
author_facet Friedrich, Ines
Bodenberger, Bernhard
Neubauer, Hannes
Hertel, Robert
Daniel, Rolf
author_sort Friedrich, Ines
title Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)
title_short Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)
title_full Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)
title_fullStr Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)
title_full_unstemmed Down in the pond: Isolation and characterization of a new Serratia marcescens strain (LVF3) from the surface water near frog’s lettuce (Groenlandia densa)
title_sort down in the pond: isolation and characterization of a new serratia marcescens strain (lvf3) from the surface water near frog’s lettuce (groenlandia densa)
publisher Public Library of Science (PLoS)
publishDate 2021
url http://dx.doi.org/10.1371/journal.pone.0259673
https://dx.plos.org/10.1371/journal.pone.0259673
genre Groenlandia
genre_facet Groenlandia
op_source PLOS ONE
volume 16, issue 11, page e0259673
ISSN 1932-6203
op_rights http://creativecommons.org/licenses/by/4.0/
op_doi https://doi.org/10.1371/journal.pone.0259673
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