Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland
The mechanisms controlling the extraordinarily slow carbon (C) mineralization rates characteristic of Sphagnum -rich peatlands (“bogs”) are not fully understood, despite decades of research on this topic. Soluble phenolic compounds have been invoked as potentially significant contributors to bog pea...
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Online Access: | http://dx.doi.org/10.1371/journal.pone.0252743 https://dx.plos.org/10.1371/journal.pone.0252743 |
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crplos:10.1371/journal.pone.0252743 2024-06-23T07:56:08+00:00 Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland Cory, Alexandra B. Chanton, Jeffrey P. Spencer, Robert G. M. Ogles, Olivia C. Rich, Virginia I. McCalley, Carmody K. Wilson, Rachel M. Riaz, Muhammad U.S. Department of Energy U.S. Department of Energy National Science Foundation 2022 http://dx.doi.org/10.1371/journal.pone.0252743 https://dx.plos.org/10.1371/journal.pone.0252743 en eng Public Library of Science (PLoS) http://creativecommons.org/licenses/by/4.0/ PLOS ONE volume 17, issue 2, page e0252743 ISSN 1932-6203 journal-article 2022 crplos https://doi.org/10.1371/journal.pone.0252743 2024-06-11T04:23:31Z The mechanisms controlling the extraordinarily slow carbon (C) mineralization rates characteristic of Sphagnum -rich peatlands (“bogs”) are not fully understood, despite decades of research on this topic. Soluble phenolic compounds have been invoked as potentially significant contributors to bog peat recalcitrance due to their affinity to slow microbial metabolism and cell growth. Despite this potentially significant role, the effects of soluble phenolic compounds on bog peat C mineralization remain unclear. We analyzed this effect by manipulating the concentration of free soluble phenolics in anaerobic bog and fen peat incubations using water-soluble polyvinylpyrrolidone (“PVP”), a compound that binds with and inactivates phenolics, preventing phenolic-enzyme interactions. CO 2 and CH 4 production rates (end-products of anaerobic C mineralization) generally correlated positively with PVP concentration following Michaelis-Menten (M.M.) saturation functions. Using M.M. parameters, we estimated that the extent to which phenolics inhibit anaerobic CO 2 production was significantly higher in the bog—62 ± 16%—than the fen—14 ± 4%. This difference was found to be more substantial with regards to methane production—wherein phenolic inhibition for the bog was estimated at 54 ± 19%, while the fen demonstrated no apparent inhibition. Consistent with this habitat difference, we observed significantly higher soluble phenolic content in bog vs. fen pore-water. Together, these findings suggest that soluble phenolics could contribute to bogs’ extraordinary recalcitrance and high (relative to other peatland habitats) CO 2 :CH 4 production ratios. Article in Journal/Newspaper permafrost PLOS PLOS ONE 17 2 e0252743 |
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English |
description |
The mechanisms controlling the extraordinarily slow carbon (C) mineralization rates characteristic of Sphagnum -rich peatlands (“bogs”) are not fully understood, despite decades of research on this topic. Soluble phenolic compounds have been invoked as potentially significant contributors to bog peat recalcitrance due to their affinity to slow microbial metabolism and cell growth. Despite this potentially significant role, the effects of soluble phenolic compounds on bog peat C mineralization remain unclear. We analyzed this effect by manipulating the concentration of free soluble phenolics in anaerobic bog and fen peat incubations using water-soluble polyvinylpyrrolidone (“PVP”), a compound that binds with and inactivates phenolics, preventing phenolic-enzyme interactions. CO 2 and CH 4 production rates (end-products of anaerobic C mineralization) generally correlated positively with PVP concentration following Michaelis-Menten (M.M.) saturation functions. Using M.M. parameters, we estimated that the extent to which phenolics inhibit anaerobic CO 2 production was significantly higher in the bog—62 ± 16%—than the fen—14 ± 4%. This difference was found to be more substantial with regards to methane production—wherein phenolic inhibition for the bog was estimated at 54 ± 19%, while the fen demonstrated no apparent inhibition. Consistent with this habitat difference, we observed significantly higher soluble phenolic content in bog vs. fen pore-water. Together, these findings suggest that soluble phenolics could contribute to bogs’ extraordinary recalcitrance and high (relative to other peatland habitats) CO 2 :CH 4 production ratios. |
author2 |
Riaz, Muhammad U.S. Department of Energy U.S. Department of Energy National Science Foundation |
format |
Article in Journal/Newspaper |
author |
Cory, Alexandra B. Chanton, Jeffrey P. Spencer, Robert G. M. Ogles, Olivia C. Rich, Virginia I. McCalley, Carmody K. Wilson, Rachel M. |
spellingShingle |
Cory, Alexandra B. Chanton, Jeffrey P. Spencer, Robert G. M. Ogles, Olivia C. Rich, Virginia I. McCalley, Carmody K. Wilson, Rachel M. Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
author_facet |
Cory, Alexandra B. Chanton, Jeffrey P. Spencer, Robert G. M. Ogles, Olivia C. Rich, Virginia I. McCalley, Carmody K. Wilson, Rachel M. |
author_sort |
Cory, Alexandra B. |
title |
Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
title_short |
Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
title_full |
Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
title_fullStr |
Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
title_full_unstemmed |
Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
title_sort |
quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
http://dx.doi.org/10.1371/journal.pone.0252743 https://dx.plos.org/10.1371/journal.pone.0252743 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
PLOS ONE volume 17, issue 2, page e0252743 ISSN 1932-6203 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1371/journal.pone.0252743 |
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PLOS ONE |
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17 |
container_issue |
2 |
container_start_page |
e0252743 |
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1802649041747574784 |