A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva
Some organisms in nature have developed the ability to enter a state of suspended metabolism called cryptobiosis when environmental conditions are unfavorable. This state-transition requires execution of a combination of genetic and biochemical pathways that enable the organism to survive for prolon...
Published in: | PLOS Genetics |
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Language: | English |
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Online Access: | http://dx.doi.org/10.1371/journal.pgen.1010798 https://dx.plos.org/10.1371/journal.pgen.1010798 |
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crplos:10.1371/journal.pgen.1010798 2024-09-15T18:29:55+00:00 A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva Shatilovich, Anastasia Gade, Vamshidhar R. Pippel, Martin Hoffmeyer, Tarja T. Tchesunov, Alexei V. Stevens, Lewis Winkler, Sylke Hughes, Graham M. Traikov, Sofia Hiller, Michael Rivkina, Elizaveta Schiffer, Philipp H. Myers, Eugene W. Kurzchalia, Teymuras V. Copenhaver, Gregory P. Russian Foundation for Basic Research Volkswagen Foundation Deutsche Forschungsgemeinschaft University College Dublin 2023 http://dx.doi.org/10.1371/journal.pgen.1010798 https://dx.plos.org/10.1371/journal.pgen.1010798 en eng Public Library of Science (PLoS) http://creativecommons.org/licenses/by/4.0/ PLOS Genetics volume 19, issue 7, page e1010798 ISSN 1553-7404 journal-article 2023 crplos https://doi.org/10.1371/journal.pgen.1010798 2024-08-13T04:09:00Z Some organisms in nature have developed the ability to enter a state of suspended metabolism called cryptobiosis when environmental conditions are unfavorable. This state-transition requires execution of a combination of genetic and biochemical pathways that enable the organism to survive for prolonged periods. Recently, nematode individuals have been reanimated from Siberian permafrost after remaining in cryptobiosis. Preliminary analysis indicates that these nematodes belong to the genera Panagrolaimus and Plectus . Here, we present precise radiocarbon dating indicating that the Panagrolaimus individuals have remained in cryptobiosis since the late Pleistocene (~46,000 years). Phylogenetic inference based on our genome assembly and a detailed morphological analysis demonstrate that they belong to an undescribed species, which we named Panagrolaimus kolymaensis . Comparative genome analysis revealed that the molecular toolkit for cryptobiosis in P . kolymaensis and in C . elegans is partly orthologous. We show that biochemical mechanisms employed by these two species to survive desiccation and freezing under laboratory conditions are similar. Our experimental evidence also reveals that C . elegans dauer larvae can remain viable for longer periods in suspended animation than previously reported. Altogether, our findings demonstrate that nematodes evolved mechanisms potentially allowing them to suspend life over geological time scales. Article in Journal/Newspaper permafrost PLOS PLOS Genetics 19 7 e1010798 |
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Open Polar |
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PLOS |
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crplos |
language |
English |
description |
Some organisms in nature have developed the ability to enter a state of suspended metabolism called cryptobiosis when environmental conditions are unfavorable. This state-transition requires execution of a combination of genetic and biochemical pathways that enable the organism to survive for prolonged periods. Recently, nematode individuals have been reanimated from Siberian permafrost after remaining in cryptobiosis. Preliminary analysis indicates that these nematodes belong to the genera Panagrolaimus and Plectus . Here, we present precise radiocarbon dating indicating that the Panagrolaimus individuals have remained in cryptobiosis since the late Pleistocene (~46,000 years). Phylogenetic inference based on our genome assembly and a detailed morphological analysis demonstrate that they belong to an undescribed species, which we named Panagrolaimus kolymaensis . Comparative genome analysis revealed that the molecular toolkit for cryptobiosis in P . kolymaensis and in C . elegans is partly orthologous. We show that biochemical mechanisms employed by these two species to survive desiccation and freezing under laboratory conditions are similar. Our experimental evidence also reveals that C . elegans dauer larvae can remain viable for longer periods in suspended animation than previously reported. Altogether, our findings demonstrate that nematodes evolved mechanisms potentially allowing them to suspend life over geological time scales. |
author2 |
Copenhaver, Gregory P. Russian Foundation for Basic Research Volkswagen Foundation Deutsche Forschungsgemeinschaft University College Dublin |
format |
Article in Journal/Newspaper |
author |
Shatilovich, Anastasia Gade, Vamshidhar R. Pippel, Martin Hoffmeyer, Tarja T. Tchesunov, Alexei V. Stevens, Lewis Winkler, Sylke Hughes, Graham M. Traikov, Sofia Hiller, Michael Rivkina, Elizaveta Schiffer, Philipp H. Myers, Eugene W. Kurzchalia, Teymuras V. |
spellingShingle |
Shatilovich, Anastasia Gade, Vamshidhar R. Pippel, Martin Hoffmeyer, Tarja T. Tchesunov, Alexei V. Stevens, Lewis Winkler, Sylke Hughes, Graham M. Traikov, Sofia Hiller, Michael Rivkina, Elizaveta Schiffer, Philipp H. Myers, Eugene W. Kurzchalia, Teymuras V. A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
author_facet |
Shatilovich, Anastasia Gade, Vamshidhar R. Pippel, Martin Hoffmeyer, Tarja T. Tchesunov, Alexei V. Stevens, Lewis Winkler, Sylke Hughes, Graham M. Traikov, Sofia Hiller, Michael Rivkina, Elizaveta Schiffer, Philipp H. Myers, Eugene W. Kurzchalia, Teymuras V. |
author_sort |
Shatilovich, Anastasia |
title |
A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
title_short |
A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
title_full |
A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
title_fullStr |
A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
title_full_unstemmed |
A novel nematode species from the Siberian permafrost shares adaptive mechanisms for cryptobiotic survival with C. elegans dauer larva |
title_sort |
novel nematode species from the siberian permafrost shares adaptive mechanisms for cryptobiotic survival with c. elegans dauer larva |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1371/journal.pgen.1010798 https://dx.plos.org/10.1371/journal.pgen.1010798 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
PLOS Genetics volume 19, issue 7, page e1010798 ISSN 1553-7404 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1371/journal.pgen.1010798 |
container_title |
PLOS Genetics |
container_volume |
19 |
container_issue |
7 |
container_start_page |
e1010798 |
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1810471407050555392 |