Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas
Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause...
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crpeerj:10.7717/peerj.9180 2024-06-23T07:52:17+00:00 Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas Potts, Robert W.A. Gutierrez, Alejandro P. Cortés-Araya, Yennifer Houston, Ross D. Bean, Tim P. Biotechnology and Biological Sciences Research Council BBSRC Institute Strategic Programme Grants Centre for Environment, Fisheries and Aquaculture Science (Cefas) Seedcorn 2020 http://dx.doi.org/10.7717/peerj.9180 https://peerj.com/articles/9180.pdf https://peerj.com/articles/9180.xml https://peerj.com/articles/9180.html en eng PeerJ https://creativecommons.org/licenses/by/4.0/ PeerJ volume 8, page e9180 ISSN 2167-8359 journal-article 2020 crpeerj https://doi.org/10.7717/peerj.9180 2024-06-11T04:28:35Z Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture of primary cells from adult Pacific oyster tissues and identify novel cell morphologies that have not been reported previously. Cultures analysed by light microscopy, qPCR, and live cell imaging demonstrated maintenance of live, metabolically active Pacific oyster cells for several weeks post-explant. Interestingly, whole hearts dissected from adult oysters were found to continue contracting rhythmically up to 8 weeks after being transferred to a tissue culture system. Mantle tissue explants were also actively moving in the culture system. These improvements in primary cell culture of bivalves may be beneficial for research in ecotoxicology, virology, immunology, and genetic resistance to disease. Article in Journal/Newspaper Crassostrea gigas Pacific oyster PeerJ Publishing Pacific PeerJ 8 e9180 |
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description |
Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture of primary cells from adult Pacific oyster tissues and identify novel cell morphologies that have not been reported previously. Cultures analysed by light microscopy, qPCR, and live cell imaging demonstrated maintenance of live, metabolically active Pacific oyster cells for several weeks post-explant. Interestingly, whole hearts dissected from adult oysters were found to continue contracting rhythmically up to 8 weeks after being transferred to a tissue culture system. Mantle tissue explants were also actively moving in the culture system. These improvements in primary cell culture of bivalves may be beneficial for research in ecotoxicology, virology, immunology, and genetic resistance to disease. |
author2 |
Biotechnology and Biological Sciences Research Council BBSRC Institute Strategic Programme Grants Centre for Environment, Fisheries and Aquaculture Science (Cefas) Seedcorn |
format |
Article in Journal/Newspaper |
author |
Potts, Robert W.A. Gutierrez, Alejandro P. Cortés-Araya, Yennifer Houston, Ross D. Bean, Tim P. |
spellingShingle |
Potts, Robert W.A. Gutierrez, Alejandro P. Cortés-Araya, Yennifer Houston, Ross D. Bean, Tim P. Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas |
author_facet |
Potts, Robert W.A. Gutierrez, Alejandro P. Cortés-Araya, Yennifer Houston, Ross D. Bean, Tim P. |
author_sort |
Potts, Robert W.A. |
title |
Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas |
title_short |
Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas |
title_full |
Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas |
title_fullStr |
Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas |
title_full_unstemmed |
Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas |
title_sort |
developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve crassostrea gigas |
publisher |
PeerJ |
publishDate |
2020 |
url |
http://dx.doi.org/10.7717/peerj.9180 https://peerj.com/articles/9180.pdf https://peerj.com/articles/9180.xml https://peerj.com/articles/9180.html |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
PeerJ volume 8, page e9180 ISSN 2167-8359 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7717/peerj.9180 |
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PeerJ |
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8 |
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e9180 |
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1802643550621401088 |