Liver slice culture as a model for lipid metabolism in fish
Hepatic lipid metabolism is traditionally investigated in vitro using hepatocyte monocultures lacking the complex three-dimensional structure and interacting cell types essential liver function. Precision cut liver slice (PCLS) culture represents an alternative in vitro system, which benefits from r...
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crpeerj:10.7717/peerj.7732 2024-09-15T17:56:27+00:00 Liver slice culture as a model for lipid metabolism in fish Harvey, Thomas N. Sandve, Simen R. Jin, Yang Vik, Jon Olav Torgersen, Jacob S. 2019 http://dx.doi.org/10.7717/peerj.7732 https://peerj.com/articles/7732.pdf https://peerj.com/articles/7732.xml https://peerj.com/articles/7732.html en eng PeerJ https://creativecommons.org/licenses/by/4.0/ PeerJ volume 7, page e7732 ISSN 2167-8359 journal-article 2019 crpeerj https://doi.org/10.7717/peerj.7732 2024-08-06T04:11:13Z Hepatic lipid metabolism is traditionally investigated in vitro using hepatocyte monocultures lacking the complex three-dimensional structure and interacting cell types essential liver function. Precision cut liver slice (PCLS) culture represents an alternative in vitro system, which benefits from retention of tissue architecture. Here, we present the first comprehensive evaluation of the PCLS method in fish (Atlantic salmon, Salmo salar L.) and validate it in the context of lipid metabolism using feeding trials, extensive transcriptomic data, and fatty acid measurements. We observe an initial period of post-slicing global transcriptome adjustment, which plateaued after 3 days in major metabolic pathways and stabilized through 9 days. PCLS fed alpha-linolenic acid (ALA) and insulin responded in a liver-like manner, increasing lipid biosynthesis gene expression. We identify interactions between insulin and ALA, where two PUFA biosynthesis genes that were induced by insulin or ALA alone, were highly down-regulated when insulin and ALA were combined. We also find that transcriptomic profiles of liver slices are exceedingly more similar to whole liver than hepatocyte monocultures, both for lipid metabolism and liver marker genes. PCLS culture opens new avenues for high throughput experimentation on the effect of “novel feed composition” and represent a promising new strategy for studying genotype-specific molecular features of metabolism. Article in Journal/Newspaper Atlantic salmon Salmo salar PeerJ Publishing PeerJ 7 e7732 |
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English |
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Hepatic lipid metabolism is traditionally investigated in vitro using hepatocyte monocultures lacking the complex three-dimensional structure and interacting cell types essential liver function. Precision cut liver slice (PCLS) culture represents an alternative in vitro system, which benefits from retention of tissue architecture. Here, we present the first comprehensive evaluation of the PCLS method in fish (Atlantic salmon, Salmo salar L.) and validate it in the context of lipid metabolism using feeding trials, extensive transcriptomic data, and fatty acid measurements. We observe an initial period of post-slicing global transcriptome adjustment, which plateaued after 3 days in major metabolic pathways and stabilized through 9 days. PCLS fed alpha-linolenic acid (ALA) and insulin responded in a liver-like manner, increasing lipid biosynthesis gene expression. We identify interactions between insulin and ALA, where two PUFA biosynthesis genes that were induced by insulin or ALA alone, were highly down-regulated when insulin and ALA were combined. We also find that transcriptomic profiles of liver slices are exceedingly more similar to whole liver than hepatocyte monocultures, both for lipid metabolism and liver marker genes. PCLS culture opens new avenues for high throughput experimentation on the effect of “novel feed composition” and represent a promising new strategy for studying genotype-specific molecular features of metabolism. |
format |
Article in Journal/Newspaper |
author |
Harvey, Thomas N. Sandve, Simen R. Jin, Yang Vik, Jon Olav Torgersen, Jacob S. |
spellingShingle |
Harvey, Thomas N. Sandve, Simen R. Jin, Yang Vik, Jon Olav Torgersen, Jacob S. Liver slice culture as a model for lipid metabolism in fish |
author_facet |
Harvey, Thomas N. Sandve, Simen R. Jin, Yang Vik, Jon Olav Torgersen, Jacob S. |
author_sort |
Harvey, Thomas N. |
title |
Liver slice culture as a model for lipid metabolism in fish |
title_short |
Liver slice culture as a model for lipid metabolism in fish |
title_full |
Liver slice culture as a model for lipid metabolism in fish |
title_fullStr |
Liver slice culture as a model for lipid metabolism in fish |
title_full_unstemmed |
Liver slice culture as a model for lipid metabolism in fish |
title_sort |
liver slice culture as a model for lipid metabolism in fish |
publisher |
PeerJ |
publishDate |
2019 |
url |
http://dx.doi.org/10.7717/peerj.7732 https://peerj.com/articles/7732.pdf https://peerj.com/articles/7732.xml https://peerj.com/articles/7732.html |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
PeerJ volume 7, page e7732 ISSN 2167-8359 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7717/peerj.7732 |
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7 |
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e7732 |
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1810432652567642112 |