Fleas of black rats ( Rattus rattus) as reservoir host of Bartonellaspp. in Chile
Background Rattus rattus is a widely distributed, invasive species that presents an important role in disease transmission, either directly or through vector arthropods such as fleas. These black rats can transmit a wide variety of pathogens, including bacteria of the genus Bartonella , which can ca...
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crpeerj:10.7717/peerj.7371 2024-09-15T18:32:00+00:00 Fleas of black rats ( Rattus rattus) as reservoir host of Bartonellaspp. in Chile Moreno Salas, Lucila Espinoza-Carniglia, Mario Lizama Schmeisser, Nicol Torres, L. Gonzalo Silva-de la Fuente, María Carolina Lareschi, Marcela González-Acuña, Daniel National Fund for Scientific and Technological Development 2019 http://dx.doi.org/10.7717/peerj.7371 https://peerj.com/articles/7371.pdf https://peerj.com/articles/7371.xml https://peerj.com/articles/7371.html en eng PeerJ https://creativecommons.org/licenses/by/4.0/ PeerJ volume 7, page e7371 ISSN 2167-8359 journal-article 2019 crpeerj https://doi.org/10.7717/peerj.7371 2024-07-09T04:08:51Z Background Rattus rattus is a widely distributed, invasive species that presents an important role in disease transmission, either directly or through vector arthropods such as fleas. These black rats can transmit a wide variety of pathogens, including bacteria of the genus Bartonella , which can cause diseases in humans and animals. In Chile, no data are available identifying fleas from synanthropic rodents as Bartonella vectors. The aim of this study was to investigate the prevalence of Bartonella spp. in the fleas of R. rattus in areas with different climate conditions and featuring different human population densities. Methods In all, 174 fleas collected from 261 R. rattus captured from 30 localities with different human densities (cities, villages, and wild areas) across five hydrographic zones of Chile (hyper-arid, arid, semi-arid, sub-humid, and hyper-humid) were examined. Bartonella spp. presence was determined through polymerase chain reaction, using gltA and rpoB genes, which were concatenated to perform a similarity analysis with BLAST and phylogenetic analysis. Results Overall, 15 fleas species were identified; Bartonella gltA and rpoB fragments were detected in 21.2% (37/174) and 19.5% (34/174) of fleas, respectively. A total of 10 of the 15 fleas species found were positive for Bartonella DNA. Leptopsylla segnis was the most commonly collected flea species ( n = 55), and it also presented a high prevalence of Bartonella DNA ( P % = 34.5%). The highest numbers of fleas of this species were collected in villages of the arid zone. There were no seasonal differences in the prevalence of Bartonella DNA. The presence of Bartonella DNA in fleas was recorded in all hydrographic areas, and the arid zone presented the highest prevalence of this species. Regarding areas with different human densities, the highest prevalence was noted in the villages (34.8% gltA and 31.8% rpoB ), followed by cities (14.8% gltA and 11.1% rpoB ) and wild areas (7.4% gltA and 14.8% rpoB ). The BLAST analysis showed a high ... Article in Journal/Newspaper Rattus rattus PeerJ Publishing PeerJ 7 e7371 |
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Background Rattus rattus is a widely distributed, invasive species that presents an important role in disease transmission, either directly or through vector arthropods such as fleas. These black rats can transmit a wide variety of pathogens, including bacteria of the genus Bartonella , which can cause diseases in humans and animals. In Chile, no data are available identifying fleas from synanthropic rodents as Bartonella vectors. The aim of this study was to investigate the prevalence of Bartonella spp. in the fleas of R. rattus in areas with different climate conditions and featuring different human population densities. Methods In all, 174 fleas collected from 261 R. rattus captured from 30 localities with different human densities (cities, villages, and wild areas) across five hydrographic zones of Chile (hyper-arid, arid, semi-arid, sub-humid, and hyper-humid) were examined. Bartonella spp. presence was determined through polymerase chain reaction, using gltA and rpoB genes, which were concatenated to perform a similarity analysis with BLAST and phylogenetic analysis. Results Overall, 15 fleas species were identified; Bartonella gltA and rpoB fragments were detected in 21.2% (37/174) and 19.5% (34/174) of fleas, respectively. A total of 10 of the 15 fleas species found were positive for Bartonella DNA. Leptopsylla segnis was the most commonly collected flea species ( n = 55), and it also presented a high prevalence of Bartonella DNA ( P % = 34.5%). The highest numbers of fleas of this species were collected in villages of the arid zone. There were no seasonal differences in the prevalence of Bartonella DNA. The presence of Bartonella DNA in fleas was recorded in all hydrographic areas, and the arid zone presented the highest prevalence of this species. Regarding areas with different human densities, the highest prevalence was noted in the villages (34.8% gltA and 31.8% rpoB ), followed by cities (14.8% gltA and 11.1% rpoB ) and wild areas (7.4% gltA and 14.8% rpoB ). The BLAST analysis showed a high ... |
author2 |
National Fund for Scientific and Technological Development |
format |
Article in Journal/Newspaper |
author |
Moreno Salas, Lucila Espinoza-Carniglia, Mario Lizama Schmeisser, Nicol Torres, L. Gonzalo Silva-de la Fuente, María Carolina Lareschi, Marcela González-Acuña, Daniel |
spellingShingle |
Moreno Salas, Lucila Espinoza-Carniglia, Mario Lizama Schmeisser, Nicol Torres, L. Gonzalo Silva-de la Fuente, María Carolina Lareschi, Marcela González-Acuña, Daniel Fleas of black rats ( Rattus rattus) as reservoir host of Bartonellaspp. in Chile |
author_facet |
Moreno Salas, Lucila Espinoza-Carniglia, Mario Lizama Schmeisser, Nicol Torres, L. Gonzalo Silva-de la Fuente, María Carolina Lareschi, Marcela González-Acuña, Daniel |
author_sort |
Moreno Salas, Lucila |
title |
Fleas of black rats ( Rattus rattus) as reservoir host of Bartonellaspp. in Chile |
title_short |
Fleas of black rats ( Rattus rattus) as reservoir host of Bartonellaspp. in Chile |
title_full |
Fleas of black rats ( Rattus rattus) as reservoir host of Bartonellaspp. in Chile |
title_fullStr |
Fleas of black rats ( Rattus rattus) as reservoir host of Bartonellaspp. in Chile |
title_full_unstemmed |
Fleas of black rats ( Rattus rattus) as reservoir host of Bartonellaspp. in Chile |
title_sort |
fleas of black rats ( rattus rattus) as reservoir host of bartonellaspp. in chile |
publisher |
PeerJ |
publishDate |
2019 |
url |
http://dx.doi.org/10.7717/peerj.7371 https://peerj.com/articles/7371.pdf https://peerj.com/articles/7371.xml https://peerj.com/articles/7371.html |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
PeerJ volume 7, page e7371 ISSN 2167-8359 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7717/peerj.7371 |
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7 |
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e7371 |
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1810473743495987200 |