Hybrid identification in Nothofagus subgenus using high resolution melting with ITS and trnL approach
The genus Nothofagus is the main component of southern South American temperate forests. The 40 Nothofagus species, evergreen and deciduous, and some natural hybrids are spread among Central and Southern Chile, Argentina, New Zealand, Australia, New Guinea and New Caledonia. Nothofagus nervosa , Not...
Published in: | PeerJ |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
PeerJ
2019
|
Subjects: | |
Online Access: | http://dx.doi.org/10.7717/peerj.6779 https://peerj.com/articles/6779.pdf https://peerj.com/articles/6779.xml https://peerj.com/articles/6779.html |
id |
crpeerj:10.7717/peerj.6779 |
---|---|
record_format |
openpolar |
spelling |
crpeerj:10.7717/peerj.6779 2024-06-02T07:56:30+00:00 Hybrid identification in Nothofagus subgenus using high resolution melting with ITS and trnL approach Solano, Jaime Anabalón, Leonardo Encina, Francisco Esse, Carlos Penneckamp, Diego 2019 http://dx.doi.org/10.7717/peerj.6779 https://peerj.com/articles/6779.pdf https://peerj.com/articles/6779.xml https://peerj.com/articles/6779.html en eng PeerJ http://creativecommons.org/licenses/by/4.0/ PeerJ volume 7, page e6779 ISSN 2167-8359 journal-article 2019 crpeerj https://doi.org/10.7717/peerj.6779 2024-05-07T14:14:10Z The genus Nothofagus is the main component of southern South American temperate forests. The 40 Nothofagus species, evergreen and deciduous, and some natural hybrids are spread among Central and Southern Chile, Argentina, New Zealand, Australia, New Guinea and New Caledonia. Nothofagus nervosa , Nothofagus obliqua and Nothofagus dombeyi are potentially very important timber producers due to their high wood quality and relative fast growth; however, indiscriminate logging has degraded vast areas the Chilean forest causing a serious state of deterioration of their genetic resource. The South of Chile has a large area covered by secondary forests of Nothofagus dombeyi . These forests have a high diversity of species, large amount of biomass and high silvicultural potential. This work shows a case of hybrid identification in Nothofagus subgenus in different secondary forests of Chile, using high resolution melting. Unknown samples of Nothofagus subgenus are genetically distinguishable with the ITS region of Nothofagus antarctica , Nothofagus nitida and N. obliqua species. It was not possible to distinguish between unknown samples of Andean versus coastal origin. Melting curves with ITS approach of unknown material are genetically similar, positioned between N. dombeyi and N. antarctica and distant from N. nitida . The unknown samples are genetically very close to Nothofagus dombeyi . This suggests the presence of hybrid individuality between species ( N. dombeyi × N. antarctica ) with the possibility of introgression towards the gene pool of N. antarctica , producing the deciduous foliage that is both present. The trnL locus has no distinction between the N. dombeyi and N. antarctica species, since a similar melting curve is present and equal Tm (80.00 °C). The trnL locus cannot be genetically distinguished from one unknown sample of Nothofagus to another, as highlighted in this study. Article in Journal/Newspaper Antarc* Antarctica PeerJ Publishing Argentina New Zealand PeerJ 7 e6779 |
institution |
Open Polar |
collection |
PeerJ Publishing |
op_collection_id |
crpeerj |
language |
English |
description |
The genus Nothofagus is the main component of southern South American temperate forests. The 40 Nothofagus species, evergreen and deciduous, and some natural hybrids are spread among Central and Southern Chile, Argentina, New Zealand, Australia, New Guinea and New Caledonia. Nothofagus nervosa , Nothofagus obliqua and Nothofagus dombeyi are potentially very important timber producers due to their high wood quality and relative fast growth; however, indiscriminate logging has degraded vast areas the Chilean forest causing a serious state of deterioration of their genetic resource. The South of Chile has a large area covered by secondary forests of Nothofagus dombeyi . These forests have a high diversity of species, large amount of biomass and high silvicultural potential. This work shows a case of hybrid identification in Nothofagus subgenus in different secondary forests of Chile, using high resolution melting. Unknown samples of Nothofagus subgenus are genetically distinguishable with the ITS region of Nothofagus antarctica , Nothofagus nitida and N. obliqua species. It was not possible to distinguish between unknown samples of Andean versus coastal origin. Melting curves with ITS approach of unknown material are genetically similar, positioned between N. dombeyi and N. antarctica and distant from N. nitida . The unknown samples are genetically very close to Nothofagus dombeyi . This suggests the presence of hybrid individuality between species ( N. dombeyi × N. antarctica ) with the possibility of introgression towards the gene pool of N. antarctica , producing the deciduous foliage that is both present. The trnL locus has no distinction between the N. dombeyi and N. antarctica species, since a similar melting curve is present and equal Tm (80.00 °C). The trnL locus cannot be genetically distinguished from one unknown sample of Nothofagus to another, as highlighted in this study. |
format |
Article in Journal/Newspaper |
author |
Solano, Jaime Anabalón, Leonardo Encina, Francisco Esse, Carlos Penneckamp, Diego |
spellingShingle |
Solano, Jaime Anabalón, Leonardo Encina, Francisco Esse, Carlos Penneckamp, Diego Hybrid identification in Nothofagus subgenus using high resolution melting with ITS and trnL approach |
author_facet |
Solano, Jaime Anabalón, Leonardo Encina, Francisco Esse, Carlos Penneckamp, Diego |
author_sort |
Solano, Jaime |
title |
Hybrid identification in Nothofagus subgenus using high resolution melting with ITS and trnL approach |
title_short |
Hybrid identification in Nothofagus subgenus using high resolution melting with ITS and trnL approach |
title_full |
Hybrid identification in Nothofagus subgenus using high resolution melting with ITS and trnL approach |
title_fullStr |
Hybrid identification in Nothofagus subgenus using high resolution melting with ITS and trnL approach |
title_full_unstemmed |
Hybrid identification in Nothofagus subgenus using high resolution melting with ITS and trnL approach |
title_sort |
hybrid identification in nothofagus subgenus using high resolution melting with its and trnl approach |
publisher |
PeerJ |
publishDate |
2019 |
url |
http://dx.doi.org/10.7717/peerj.6779 https://peerj.com/articles/6779.pdf https://peerj.com/articles/6779.xml https://peerj.com/articles/6779.html |
geographic |
Argentina New Zealand |
geographic_facet |
Argentina New Zealand |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
PeerJ volume 7, page e6779 ISSN 2167-8359 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7717/peerj.6779 |
container_title |
PeerJ |
container_volume |
7 |
container_start_page |
e6779 |
_version_ |
1800756526751678464 |