Comparative genomic analysis of a new tellurite-resistant Psychrobacterstrain isolated from the Antarctic Peninsula
The Psychrobacter genus is a cosmopolitan and diverse group of aerobic, cold-adapted, Gram-negative bacteria exhibiting biotechnological potential for low-temperature applications including bioremediation. Here, we present the draft genome sequence of a bacterium from the Psychrobacter genus isolate...
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crpeerj:10.7717/peerj.4402 2024-09-15T17:44:39+00:00 Comparative genomic analysis of a new tellurite-resistant Psychrobacterstrain isolated from the Antarctic Peninsula Muñoz-Villagrán, Claudia Melissa Mendez, Katterinne N. Cornejo, Fabian Figueroa, Maximiliano Undabarrena, Agustina Morales, Eduardo Hugo Arenas-Salinas, Mauricio Arenas, Felipe Alejandro Castro-Nallar, Eduardo Vásquez, Claudio Christian Fondecyt (Fondo Nacional de Ciencia y Tecnología) Conicyt (Comisión Nacional de Investigación Científica y Tecnológica) INACH (Instituto Antártico Chileno) Fondo de Proyectos para Investigadores Iniciales—Universidad de Talca Fondecyt de Iniciación en Investigación 2018 http://dx.doi.org/10.7717/peerj.4402 https://peerj.com/articles/4402.pdf https://peerj.com/articles/4402.xml https://peerj.com/articles/4402.html en eng PeerJ http://creativecommons.org/licenses/by/4.0/ PeerJ volume 6, page e4402 ISSN 2167-8359 journal-article 2018 crpeerj https://doi.org/10.7717/peerj.4402 2024-08-20T04:10:22Z The Psychrobacter genus is a cosmopolitan and diverse group of aerobic, cold-adapted, Gram-negative bacteria exhibiting biotechnological potential for low-temperature applications including bioremediation. Here, we present the draft genome sequence of a bacterium from the Psychrobacter genus isolated from a sediment sample from King George Island, Antarctica (3,490,622 bp; 18 scaffolds; G + C = 42.76%). Using phylogenetic analysis, biochemical properties and scanning electron microscopy the bacterium was identified as Psychrobacter glacincola BNF20, making it the first genome sequence reported for this species. P. glacincola BNF20 showed high tellurite (MIC 2.3 mM) and chromate (MIC 6.0 mM) resistance, respectively. Genome-wide nucleotide identity comparisons revealed that P. glacincola BNF20 is highly similar (>90%) to other uncharacterized Psychrobacter spp. such as JCM18903, JCM18902, and P11F6. Bayesian multi-locus phylogenetic analysis showed that P. glacincola BNF20 belongs to a polyphyletic clade with other bacteria isolated from polar regions. A high number of genes related to metal(loid) resistance were found, including tellurite resistance genetic determinants located in two contigs: Contig LIQB01000002.1 exhibited five ter genes, each showing putative promoter sequences (terACDEZ), whereas contig LIQB1000003.2 showed a variant of the terZ gene. Finally, investigating the presence and taxonomic distribution of ter genes in the NCBI’s RefSeq bacterial database (5,398 genomes, as January 2017), revealed that 2,623 (48.59%) genomes showed at least one ter gene. At the family level, most (68.7%) genomes harbored one ter gene and 15.6% exhibited five (including P. glacincola BNF20). Overall, our results highlight the diverse nature (genetic and geographic diversity) of the Psychrobacter genus, provide insights into potential mechanisms of metal resistance, and exemplify the benefits of sampling remote locations for prospecting new molecular determinants. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica King George Island PeerJ Publishing PeerJ 6 e4402 |
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Open Polar |
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PeerJ Publishing |
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crpeerj |
language |
English |
description |
The Psychrobacter genus is a cosmopolitan and diverse group of aerobic, cold-adapted, Gram-negative bacteria exhibiting biotechnological potential for low-temperature applications including bioremediation. Here, we present the draft genome sequence of a bacterium from the Psychrobacter genus isolated from a sediment sample from King George Island, Antarctica (3,490,622 bp; 18 scaffolds; G + C = 42.76%). Using phylogenetic analysis, biochemical properties and scanning electron microscopy the bacterium was identified as Psychrobacter glacincola BNF20, making it the first genome sequence reported for this species. P. glacincola BNF20 showed high tellurite (MIC 2.3 mM) and chromate (MIC 6.0 mM) resistance, respectively. Genome-wide nucleotide identity comparisons revealed that P. glacincola BNF20 is highly similar (>90%) to other uncharacterized Psychrobacter spp. such as JCM18903, JCM18902, and P11F6. Bayesian multi-locus phylogenetic analysis showed that P. glacincola BNF20 belongs to a polyphyletic clade with other bacteria isolated from polar regions. A high number of genes related to metal(loid) resistance were found, including tellurite resistance genetic determinants located in two contigs: Contig LIQB01000002.1 exhibited five ter genes, each showing putative promoter sequences (terACDEZ), whereas contig LIQB1000003.2 showed a variant of the terZ gene. Finally, investigating the presence and taxonomic distribution of ter genes in the NCBI’s RefSeq bacterial database (5,398 genomes, as January 2017), revealed that 2,623 (48.59%) genomes showed at least one ter gene. At the family level, most (68.7%) genomes harbored one ter gene and 15.6% exhibited five (including P. glacincola BNF20). Overall, our results highlight the diverse nature (genetic and geographic diversity) of the Psychrobacter genus, provide insights into potential mechanisms of metal resistance, and exemplify the benefits of sampling remote locations for prospecting new molecular determinants. |
author2 |
Fondecyt (Fondo Nacional de Ciencia y Tecnología) Conicyt (Comisión Nacional de Investigación Científica y Tecnológica) INACH (Instituto Antártico Chileno) Fondo de Proyectos para Investigadores Iniciales—Universidad de Talca Fondecyt de Iniciación en Investigación |
format |
Article in Journal/Newspaper |
author |
Muñoz-Villagrán, Claudia Melissa Mendez, Katterinne N. Cornejo, Fabian Figueroa, Maximiliano Undabarrena, Agustina Morales, Eduardo Hugo Arenas-Salinas, Mauricio Arenas, Felipe Alejandro Castro-Nallar, Eduardo Vásquez, Claudio Christian |
spellingShingle |
Muñoz-Villagrán, Claudia Melissa Mendez, Katterinne N. Cornejo, Fabian Figueroa, Maximiliano Undabarrena, Agustina Morales, Eduardo Hugo Arenas-Salinas, Mauricio Arenas, Felipe Alejandro Castro-Nallar, Eduardo Vásquez, Claudio Christian Comparative genomic analysis of a new tellurite-resistant Psychrobacterstrain isolated from the Antarctic Peninsula |
author_facet |
Muñoz-Villagrán, Claudia Melissa Mendez, Katterinne N. Cornejo, Fabian Figueroa, Maximiliano Undabarrena, Agustina Morales, Eduardo Hugo Arenas-Salinas, Mauricio Arenas, Felipe Alejandro Castro-Nallar, Eduardo Vásquez, Claudio Christian |
author_sort |
Muñoz-Villagrán, Claudia Melissa |
title |
Comparative genomic analysis of a new tellurite-resistant Psychrobacterstrain isolated from the Antarctic Peninsula |
title_short |
Comparative genomic analysis of a new tellurite-resistant Psychrobacterstrain isolated from the Antarctic Peninsula |
title_full |
Comparative genomic analysis of a new tellurite-resistant Psychrobacterstrain isolated from the Antarctic Peninsula |
title_fullStr |
Comparative genomic analysis of a new tellurite-resistant Psychrobacterstrain isolated from the Antarctic Peninsula |
title_full_unstemmed |
Comparative genomic analysis of a new tellurite-resistant Psychrobacterstrain isolated from the Antarctic Peninsula |
title_sort |
comparative genomic analysis of a new tellurite-resistant psychrobacterstrain isolated from the antarctic peninsula |
publisher |
PeerJ |
publishDate |
2018 |
url |
http://dx.doi.org/10.7717/peerj.4402 https://peerj.com/articles/4402.pdf https://peerj.com/articles/4402.xml https://peerj.com/articles/4402.html |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica King George Island |
op_source |
PeerJ volume 6, page e4402 ISSN 2167-8359 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7717/peerj.4402 |
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PeerJ |
container_volume |
6 |
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e4402 |
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1810492299410407424 |