Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonassp strain AMS3
A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular we...
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crpeerj:10.7717/peerj.2420 2024-09-15T17:44:00+00:00 Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonassp strain AMS3 Latip, Wahhida Raja Abd Rahman, Raja Noor Zaliha Chor Leow, Adam Thean Mohd Shariff, Fairolniza Mohamad Ali, Mohd Shukuri University Putra Malaysia 2016 http://dx.doi.org/10.7717/peerj.2420 https://peerj.com/articles/2420.pdf https://peerj.com/articles/2420.xml https://peerj.com/articles/2420.html en eng PeerJ http://creativecommons.org/licenses/by/4.0/ PeerJ volume 4, page e2420 ISSN 2167-8359 journal-article 2016 crpeerj https://doi.org/10.7717/peerj.2420 2024-06-25T04:09:46Z A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular weight was approximately ∼60 kDa including the strep and affinity tags. Interestingly, the purified Antarctic AMS3 lipase exhibited broad temperature profile from 10–70 °C and stable over a broad pH range from 5.0 to pH 10.0. Various mono and divalent metal ions increased the activity of the AMS3 lipase, but Ni 2+ decreased its activity. The purified lipase exhibited the highest activity in the presence of sunflower oil. In addition, the enzyme activity in 25% v/v solvents at 50 °C particularly to n-hexane, DMSO and methanol could be useful for catalysis reaction in organic solvent and at broad temperature. Article in Journal/Newspaper Antarc* Antarctic PeerJ Publishing PeerJ 4 e2420 |
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English |
description |
A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular weight was approximately ∼60 kDa including the strep and affinity tags. Interestingly, the purified Antarctic AMS3 lipase exhibited broad temperature profile from 10–70 °C and stable over a broad pH range from 5.0 to pH 10.0. Various mono and divalent metal ions increased the activity of the AMS3 lipase, but Ni 2+ decreased its activity. The purified lipase exhibited the highest activity in the presence of sunflower oil. In addition, the enzyme activity in 25% v/v solvents at 50 °C particularly to n-hexane, DMSO and methanol could be useful for catalysis reaction in organic solvent and at broad temperature. |
author2 |
University Putra Malaysia |
format |
Article in Journal/Newspaper |
author |
Latip, Wahhida Raja Abd Rahman, Raja Noor Zaliha Chor Leow, Adam Thean Mohd Shariff, Fairolniza Mohamad Ali, Mohd Shukuri |
spellingShingle |
Latip, Wahhida Raja Abd Rahman, Raja Noor Zaliha Chor Leow, Adam Thean Mohd Shariff, Fairolniza Mohamad Ali, Mohd Shukuri Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonassp strain AMS3 |
author_facet |
Latip, Wahhida Raja Abd Rahman, Raja Noor Zaliha Chor Leow, Adam Thean Mohd Shariff, Fairolniza Mohamad Ali, Mohd Shukuri |
author_sort |
Latip, Wahhida |
title |
Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonassp strain AMS3 |
title_short |
Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonassp strain AMS3 |
title_full |
Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonassp strain AMS3 |
title_fullStr |
Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonassp strain AMS3 |
title_full_unstemmed |
Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonassp strain AMS3 |
title_sort |
expression and characterization of thermotolerant lipase with broad ph profiles isolated from an antarctic pseudomonassp strain ams3 |
publisher |
PeerJ |
publishDate |
2016 |
url |
http://dx.doi.org/10.7717/peerj.2420 https://peerj.com/articles/2420.pdf https://peerj.com/articles/2420.xml https://peerj.com/articles/2420.html |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
PeerJ volume 4, page e2420 ISSN 2167-8359 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7717/peerj.2420 |
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PeerJ |
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4 |
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e2420 |
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1810491268212457472 |