Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica
Considering the industrial interest of biodegradable polymer poly-3-hydroxybutyrate (PHB), the marine bacteria Neptunomonas antarctica was studied for its ability to accumulate PHB. The extracted polymer was confirmed to be PHB by nuclear magnetic resonance analysis. In shake flask cultures using na...
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crpeerj:10.7717/peerj.2291 2024-06-23T07:46:00+00:00 Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica Liu, Xiao-Jie Zhang, Jie Hong, Peng-Hui Li, Zheng-Jun National Natural Science Foundation of China National Science & Technology Pillar Program of China Beijing Higher Education Young Elite Teacher Project 2016 http://dx.doi.org/10.7717/peerj.2291 https://peerj.com/articles/2291.pdf https://peerj.com/articles/2291.xml https://peerj.com/articles/2291.html en eng PeerJ http://creativecommons.org/licenses/by/4.0/ PeerJ volume 4, page e2291 ISSN 2167-8359 journal-article 2016 crpeerj https://doi.org/10.7717/peerj.2291 2024-06-04T06:21:59Z Considering the industrial interest of biodegradable polymer poly-3-hydroxybutyrate (PHB), the marine bacteria Neptunomonas antarctica was studied for its ability to accumulate PHB. The extracted polymer was confirmed to be PHB by nuclear magnetic resonance analysis. In shake flask cultures using natural seawater as medium components, PHB was produced up to 2.12 g/L with a yield of 0.18 g PHB/g fructose. In the presence of artificial seawater, the PHB titer and yield reached 2.13 g/L and 0.13 g PHB/g fructose, respectively. The accumulated polymer gradually decreased when fructose was exhausted, indicating that intracellular PHB was degraded by N. antarctica . The weight-average and number-average molecular weights of PHB produced within natural seawater were 2.4 × 10 5 g/mol and 1.7 × 10 5 g/mol, respectively. Our results highlight the potential of N. antarctica for PHB production with seawater as a nutrient source. Article in Journal/Newspaper Antarc* Antarctica PeerJ Publishing PeerJ 4 e2291 |
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PeerJ Publishing |
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English |
description |
Considering the industrial interest of biodegradable polymer poly-3-hydroxybutyrate (PHB), the marine bacteria Neptunomonas antarctica was studied for its ability to accumulate PHB. The extracted polymer was confirmed to be PHB by nuclear magnetic resonance analysis. In shake flask cultures using natural seawater as medium components, PHB was produced up to 2.12 g/L with a yield of 0.18 g PHB/g fructose. In the presence of artificial seawater, the PHB titer and yield reached 2.13 g/L and 0.13 g PHB/g fructose, respectively. The accumulated polymer gradually decreased when fructose was exhausted, indicating that intracellular PHB was degraded by N. antarctica . The weight-average and number-average molecular weights of PHB produced within natural seawater were 2.4 × 10 5 g/mol and 1.7 × 10 5 g/mol, respectively. Our results highlight the potential of N. antarctica for PHB production with seawater as a nutrient source. |
author2 |
National Natural Science Foundation of China National Science & Technology Pillar Program of China Beijing Higher Education Young Elite Teacher Project |
format |
Article in Journal/Newspaper |
author |
Liu, Xiao-Jie Zhang, Jie Hong, Peng-Hui Li, Zheng-Jun |
spellingShingle |
Liu, Xiao-Jie Zhang, Jie Hong, Peng-Hui Li, Zheng-Jun Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
author_facet |
Liu, Xiao-Jie Zhang, Jie Hong, Peng-Hui Li, Zheng-Jun |
author_sort |
Liu, Xiao-Jie |
title |
Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
title_short |
Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
title_full |
Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
title_fullStr |
Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
title_full_unstemmed |
Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
title_sort |
microbial production and characterization of poly-3-hydroxybutyrate by neptunomonas antarctica |
publisher |
PeerJ |
publishDate |
2016 |
url |
http://dx.doi.org/10.7717/peerj.2291 https://peerj.com/articles/2291.pdf https://peerj.com/articles/2291.xml https://peerj.com/articles/2291.html |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
PeerJ volume 4, page e2291 ISSN 2167-8359 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7717/peerj.2291 |
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PeerJ |
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4 |
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e2291 |
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1802643489200013312 |