Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno
The key part of creating bone material for teaching is degreasing and whitening it. However, the substances used are often dangerous and toxic. We tested and compared safer methods based on two physical variables. These are light and heat. The material for our study was 45 femurs from 23 adult domes...
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crpeerj:10.7717/peerj.14036 2024-06-02T08:05:03+00:00 Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno Horák, Ondřej Pyszko, Martin Páral, Václav Šandor, Ondřej The University of Veterinary Sciences Brno paid the APC for this article 2022 http://dx.doi.org/10.7717/peerj.14036 https://peerj.com/articles/14036.pdf https://peerj.com/articles/14036.xml https://peerj.com/articles/14036.html en eng PeerJ https://creativecommons.org/licenses/by/4.0/ PeerJ volume 10, page e14036 ISSN 2167-8359 journal-article 2022 crpeerj https://doi.org/10.7717/peerj.14036 2024-05-07T14:14:19Z The key part of creating bone material for teaching is degreasing and whitening it. However, the substances used are often dangerous and toxic. We tested and compared safer methods based on two physical variables. These are light and heat. The material for our study was 45 femurs from 23 adult domestic dogs ( Canis lupus f. familiaris ). The bones were divided into three groups of 15 pieces according to the method used to remove muscles and ligaments from their surface. Five femurs from each group were exposed to three different light sources for 28 days—sunlight, warm light from a classical incandescent light bulb and cold light by a LED bulb. At regular intervals, the change in the colour of the bone surface and the amount of fat loss from the medullary cavity was also monitored. The best degreasing and bleaching results were achieved in macerated bones exposed to sunlight. They achieved the required condition as early as 21 days after the start of sun exposure. The biggest problem was haemoglobin, which permeated through the Haversian canals and discoloured the bone tissue. The results showed that the use of light and heat is a suitable and safe alternative to chemical methods of degreasing and bleaching bones. The disadvantage is the length of time, especially for native material. Article in Journal/Newspaper Canis lupus PeerJ Publishing PeerJ 10 e14036 |
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The key part of creating bone material for teaching is degreasing and whitening it. However, the substances used are often dangerous and toxic. We tested and compared safer methods based on two physical variables. These are light and heat. The material for our study was 45 femurs from 23 adult domestic dogs ( Canis lupus f. familiaris ). The bones were divided into three groups of 15 pieces according to the method used to remove muscles and ligaments from their surface. Five femurs from each group were exposed to three different light sources for 28 days—sunlight, warm light from a classical incandescent light bulb and cold light by a LED bulb. At regular intervals, the change in the colour of the bone surface and the amount of fat loss from the medullary cavity was also monitored. The best degreasing and bleaching results were achieved in macerated bones exposed to sunlight. They achieved the required condition as early as 21 days after the start of sun exposure. The biggest problem was haemoglobin, which permeated through the Haversian canals and discoloured the bone tissue. The results showed that the use of light and heat is a suitable and safe alternative to chemical methods of degreasing and bleaching bones. The disadvantage is the length of time, especially for native material. |
author2 |
The University of Veterinary Sciences Brno paid the APC for this article |
format |
Article in Journal/Newspaper |
author |
Horák, Ondřej Pyszko, Martin Páral, Václav Šandor, Ondřej |
spellingShingle |
Horák, Ondřej Pyszko, Martin Páral, Václav Šandor, Ondřej Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
author_facet |
Horák, Ondřej Pyszko, Martin Páral, Václav Šandor, Ondřej |
author_sort |
Horák, Ondřej |
title |
Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
title_short |
Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
title_full |
Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
title_fullStr |
Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
title_full_unstemmed |
Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
title_sort |
degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the university of veterinary sciences brno |
publisher |
PeerJ |
publishDate |
2022 |
url |
http://dx.doi.org/10.7717/peerj.14036 https://peerj.com/articles/14036.pdf https://peerj.com/articles/14036.xml https://peerj.com/articles/14036.html |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
PeerJ volume 10, page e14036 ISSN 2167-8359 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7717/peerj.14036 |
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PeerJ |
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10 |
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e14036 |
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1800749795397074944 |