Unearthing soil arthropod diversity through DNA metabarcoding
DNA metabarcoding has the potential to greatly advance understanding of soil biodiversity, but this approach has seen limited application for the most abundant and species-rich group of soil fauna–the arthropods. This study begins to address this gap by comparing information on species composition r...
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crpeerj:10.7717/peerj.12845 2024-10-06T13:46:17+00:00 Unearthing soil arthropod diversity through DNA metabarcoding Young, Monica R. Hebert, Paul D. N. NSERC Discovery Grant Ontario Ministry of Research, Innovation and Science, Polar Knowledge Canada Canada Foundation for Innovation Canada C3 Initiative Northern Scientific Training Program NSERC’s Canada Graduate Scholarship Canada First Research Excellence Fund 2022 http://dx.doi.org/10.7717/peerj.12845 https://peerj.com/articles/12845.pdf https://peerj.com/articles/12845.xml https://peerj.com/articles/12845.html en eng PeerJ https://creativecommons.org/licenses/by/4.0/ PeerJ volume 10, page e12845 ISSN 2167-8359 journal-article 2022 crpeerj https://doi.org/10.7717/peerj.12845 2024-09-09T06:00:24Z DNA metabarcoding has the potential to greatly advance understanding of soil biodiversity, but this approach has seen limited application for the most abundant and species-rich group of soil fauna–the arthropods. This study begins to address this gap by comparing information on species composition recovered from metabarcoding two types of bulk samples (specimens, soil) from a temperate zone site and from bulk soil samples collected at eight sites in the Arctic. Analysis of 22 samples (3 specimen, 19 soil) revealed 410 arthropod OTUs belonging to 112 families, 25 orders, and nine classes. Studies at the temperate zone site revealed little overlap in species composition between soil and specimen samples, but more overlap at higher taxonomic levels (families, orders) and congruent patterns of α - and β -diversity. Expansion of soil analyses to the Arctic revealed locally rich, highly dissimilar, and spatially structured assemblages compatible with dispersal limited and environmentally driven assembly. The current study demonstrates that DNA metabarcoding of bulk soil enables rapid, large-scale assessments of soil arthropod diversity. However, deep sequence coverage is required to adequately capture the species present in these samples, and expansion of the DNA barcode reference library is necessary to improve taxonomic resolution of the sequences recovered through this approach. Article in Journal/Newspaper Arctic PeerJ Publishing Arctic PeerJ 10 e12845 |
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English |
description |
DNA metabarcoding has the potential to greatly advance understanding of soil biodiversity, but this approach has seen limited application for the most abundant and species-rich group of soil fauna–the arthropods. This study begins to address this gap by comparing information on species composition recovered from metabarcoding two types of bulk samples (specimens, soil) from a temperate zone site and from bulk soil samples collected at eight sites in the Arctic. Analysis of 22 samples (3 specimen, 19 soil) revealed 410 arthropod OTUs belonging to 112 families, 25 orders, and nine classes. Studies at the temperate zone site revealed little overlap in species composition between soil and specimen samples, but more overlap at higher taxonomic levels (families, orders) and congruent patterns of α - and β -diversity. Expansion of soil analyses to the Arctic revealed locally rich, highly dissimilar, and spatially structured assemblages compatible with dispersal limited and environmentally driven assembly. The current study demonstrates that DNA metabarcoding of bulk soil enables rapid, large-scale assessments of soil arthropod diversity. However, deep sequence coverage is required to adequately capture the species present in these samples, and expansion of the DNA barcode reference library is necessary to improve taxonomic resolution of the sequences recovered through this approach. |
author2 |
NSERC Discovery Grant Ontario Ministry of Research, Innovation and Science, Polar Knowledge Canada Canada Foundation for Innovation Canada C3 Initiative Northern Scientific Training Program NSERC’s Canada Graduate Scholarship Canada First Research Excellence Fund |
format |
Article in Journal/Newspaper |
author |
Young, Monica R. Hebert, Paul D. N. |
spellingShingle |
Young, Monica R. Hebert, Paul D. N. Unearthing soil arthropod diversity through DNA metabarcoding |
author_facet |
Young, Monica R. Hebert, Paul D. N. |
author_sort |
Young, Monica R. |
title |
Unearthing soil arthropod diversity through DNA metabarcoding |
title_short |
Unearthing soil arthropod diversity through DNA metabarcoding |
title_full |
Unearthing soil arthropod diversity through DNA metabarcoding |
title_fullStr |
Unearthing soil arthropod diversity through DNA metabarcoding |
title_full_unstemmed |
Unearthing soil arthropod diversity through DNA metabarcoding |
title_sort |
unearthing soil arthropod diversity through dna metabarcoding |
publisher |
PeerJ |
publishDate |
2022 |
url |
http://dx.doi.org/10.7717/peerj.12845 https://peerj.com/articles/12845.pdf https://peerj.com/articles/12845.xml https://peerj.com/articles/12845.html |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PeerJ volume 10, page e12845 ISSN 2167-8359 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7717/peerj.12845 |
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PeerJ |
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10 |
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e12845 |
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1812174584565202944 |