Investigation of lipolytic activity of the red king crab hepatopancreas homogenate by NMR spectroscopy

• Published in: PeerJ
• Main Authors: Timchenko, Maria, Molchanov, Vladislav, Molchanov, Maxim, Timchenko, Alexander, Sogorin, Evgeny
• Format: Article in Journal/Newspaper
• Language: English
• Published: PeerJ 2022
• Subjects: Paralithodes camtschaticus; Red king crab
• Online Access: http://dx.doi.org/10.7717/peerj.12742; https://peerj.com/articles/12742.pdf; https://peerj.com/articles/12742.xml; https://peerj.com/articles/12742.html

The digestive gland of craboids (hepatopancreas) is rich in a huge number of various enzymes (collagenases, nucleases, hyaluronidases, proteases), which are well studied at the moment. However, little is known about crustacean lipases. In this work, using 1 H NMR spectroscopy, it was found that the hepatopancreas homogenate of the red king crab Paralithodes camtschaticus demonstrates high lipolytic activity against triacetin in a wide pH range and shows moderate activity against the caprylic/capric triglyceride emulsion. Under the action of the hepatopancreas homogenate, triacylglycerols are converted into 1,2-diacylglycerol, and then into 2-monoacylglycerol and 1-monoacylglycerol. The 1-monoacylglycerol predominates in the reaction products. The use of NMR spectroscopy makes it possible to quickly detect hydrolysis products and evaluate the reaction direction.