The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment
Numerous bacteria entered the viable but non-culturable state due to the stresses of dry and salt in soils. YeaZ of Gram-negative bacteria is a resuscitation promoting factor (Rpf) homologous protein could resuscitate bacteria of natural environment in VBNC state. To investigate the promoting effect...
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crpeerj:10.7717/peerj.10342 2024-06-02T07:56:51+00:00 The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment Li, Yanlin Chen, Jixiang Wang, Yonggang Ma, Dan Rui, Wenhong National Natural Science Foundation of China Petrochina Beijing Gas Pipeline Co Ltd. Scientific research project Scientific and Technological Research Program of Chongqing Municipal Education Commission 2020 http://dx.doi.org/10.7717/peerj.10342 https://peerj.com/articles/10342.pdf https://peerj.com/articles/10342.xml https://peerj.com/articles/10342.html en eng PeerJ https://creativecommons.org/licenses/by/4.0/ PeerJ volume 8, page e10342 ISSN 2167-8359 journal-article 2020 crpeerj https://doi.org/10.7717/peerj.10342 2024-05-07T14:13:35Z Numerous bacteria entered the viable but non-culturable state due to the stresses of dry and salt in soils. YeaZ of Gram-negative bacteria is a resuscitation promoting factor (Rpf) homologous protein could resuscitate bacteria of natural environment in VBNC state. To investigate the promoting effect of YeaZ on the isolation of viable but non-culturable (VBNC) bacteria from soil samples in extreme environments, the recombinant YeaZ of Vibrio harveyi was prepared and added to the soil samples from volcanic soil and saline soil in Northwest China. The study has shown that YeaZ can promote the recovery and growth of soil microorganisms, and the number of cultivable bacteria in volcanic and saline soil has increased from 0.17 × 10 3 and 2.03 × 10 3 cfu⋅ml −1 to 1.00 × 10 3 and 5.55 × 10 3 cfu⋅ml −1 , respectively. The 16S rDNA gene sequencing and phylogenetic analysis showed that YeaZ played an essential role in the increase of composition and diversity of bacteria. A total of 13 bacterial strains were isolated from the volcanic soil samples, which belong to phyla Actinobacteria, Firmicutes and Gamma-proteobacteria. Four species, including Ornithinimicrobium kibberense , Agrococcus citreus , Stenotrophomonas rhizophila and Pseudomonas zhaodongensis were found in the control group, while Micrococcus antarcticus , Kocuria rose , Salinibacterium xinjiangense , Planococcus antarcticus , Ornithinimicrobium kibberense and Pseudomonas zhaodongensis were isolated from the treatment groups (addition of YeaZ). Twenty-one strains were isolated from the saline soil samples, including eight species from the control group and thirteen species from the treatment groups, among which nine species were only found, including Bacillus oceanisediminis , Brevibacillus brevis , Paenibacillus xylanilyticus , Microbacterium maritypicum , B. subtilis , B. alcalophilus , B. niabensis , Oceanimonas doudoroffii and Zobellella taiwanensis . The results suggest that addition of YeaZ to soil samples can promote the recovery of VBNC. This method has ... Article in Journal/Newspaper Antarc* antarcticus PeerJ Publishing PeerJ 8 e10342 |
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English |
description |
Numerous bacteria entered the viable but non-culturable state due to the stresses of dry and salt in soils. YeaZ of Gram-negative bacteria is a resuscitation promoting factor (Rpf) homologous protein could resuscitate bacteria of natural environment in VBNC state. To investigate the promoting effect of YeaZ on the isolation of viable but non-culturable (VBNC) bacteria from soil samples in extreme environments, the recombinant YeaZ of Vibrio harveyi was prepared and added to the soil samples from volcanic soil and saline soil in Northwest China. The study has shown that YeaZ can promote the recovery and growth of soil microorganisms, and the number of cultivable bacteria in volcanic and saline soil has increased from 0.17 × 10 3 and 2.03 × 10 3 cfu⋅ml −1 to 1.00 × 10 3 and 5.55 × 10 3 cfu⋅ml −1 , respectively. The 16S rDNA gene sequencing and phylogenetic analysis showed that YeaZ played an essential role in the increase of composition and diversity of bacteria. A total of 13 bacterial strains were isolated from the volcanic soil samples, which belong to phyla Actinobacteria, Firmicutes and Gamma-proteobacteria. Four species, including Ornithinimicrobium kibberense , Agrococcus citreus , Stenotrophomonas rhizophila and Pseudomonas zhaodongensis were found in the control group, while Micrococcus antarcticus , Kocuria rose , Salinibacterium xinjiangense , Planococcus antarcticus , Ornithinimicrobium kibberense and Pseudomonas zhaodongensis were isolated from the treatment groups (addition of YeaZ). Twenty-one strains were isolated from the saline soil samples, including eight species from the control group and thirteen species from the treatment groups, among which nine species were only found, including Bacillus oceanisediminis , Brevibacillus brevis , Paenibacillus xylanilyticus , Microbacterium maritypicum , B. subtilis , B. alcalophilus , B. niabensis , Oceanimonas doudoroffii and Zobellella taiwanensis . The results suggest that addition of YeaZ to soil samples can promote the recovery of VBNC. This method has ... |
author2 |
National Natural Science Foundation of China Petrochina Beijing Gas Pipeline Co Ltd. Scientific research project Scientific and Technological Research Program of Chongqing Municipal Education Commission |
format |
Article in Journal/Newspaper |
author |
Li, Yanlin Chen, Jixiang Wang, Yonggang Ma, Dan Rui, Wenhong |
spellingShingle |
Li, Yanlin Chen, Jixiang Wang, Yonggang Ma, Dan Rui, Wenhong The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment |
author_facet |
Li, Yanlin Chen, Jixiang Wang, Yonggang Ma, Dan Rui, Wenhong |
author_sort |
Li, Yanlin |
title |
The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment |
title_short |
The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment |
title_full |
The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment |
title_fullStr |
The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment |
title_full_unstemmed |
The effects of the recombinant YeaZ of Vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment |
title_sort |
effects of the recombinant yeaz of vibrio harveyi on the resuscitation and growth of soil bacteria in extreme soil environment |
publisher |
PeerJ |
publishDate |
2020 |
url |
http://dx.doi.org/10.7717/peerj.10342 https://peerj.com/articles/10342.pdf https://peerj.com/articles/10342.xml https://peerj.com/articles/10342.html |
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Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_source |
PeerJ volume 8, page e10342 ISSN 2167-8359 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7717/peerj.10342 |
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e10342 |
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1800759589893832704 |