Spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves
Lipids are an integral part of cellular membranes that allow cells to alter stiffness, permeability, and curvature. Among the diversity of lipids, phosphonolipids uniquely contain a phosphonate bond between carbon and phosphorous. Despite this distinctive biochemical characteristic, few studies have...
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crpeerj:10.7717/peerj-achem.21 2024-09-15T18:03:14+00:00 Spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves Bourceau, Patric Michellod, Dolma Geier, Benedikt Liebeke, Manuel The Max Planck Society The MARUM Cluster of Excellence ‘The Ocean Floor’ (Deutsche Forschungsgemeinschaft European Research Council Advanced Grant 2022 http://dx.doi.org/10.7717/peerj-achem.21 https://peerj.com/articles/achem-21.pdf https://peerj.com/articles/achem-21.xml https://peerj.com/articles/achem-21.html en eng PeerJ https://creativecommons.org/licenses/by/4.0/ PeerJ Analytical Chemistry volume 4, page e21 ISSN 2691-6630 journal-article 2022 crpeerj https://doi.org/10.7717/peerj-achem.21 2024-08-20T04:10:22Z Lipids are an integral part of cellular membranes that allow cells to alter stiffness, permeability, and curvature. Among the diversity of lipids, phosphonolipids uniquely contain a phosphonate bond between carbon and phosphorous. Despite this distinctive biochemical characteristic, few studies have explored the biological role of phosphonolipids, although a protective function has been inferred based on chemical and biological stability. We analyzed two species of marine mollusks, the blue mussel Mytilus edulis and pacific oyster Crassostrea gigas , and determined the diversity of phosphonolipids and their distribution in different organs. High-resolution spatial metabolomics revealed that the lipidome varies significantly between tissues within one organ. Despite their chemical similarity, we observed a high heterogeneity of phosphonolipid distributions that originated from minor structural differences. Some phosphonolipids are ubiquitously distributed, while others are present almost exclusively in the layer of ciliated epithelial cells. This distinct localization of certain phosphonolipids in tissues exposed to the environment could support the hypothesis of a protective function in mollusks. This study highlights that the tissue specific distribution of an individual metabolite can be a valuable tool for inferring its function and guiding functional analyses. Article in Journal/Newspaper Crassostrea gigas Pacific oyster PeerJ Publishing PeerJ Analytical Chemistry 4 e21 |
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English |
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Lipids are an integral part of cellular membranes that allow cells to alter stiffness, permeability, and curvature. Among the diversity of lipids, phosphonolipids uniquely contain a phosphonate bond between carbon and phosphorous. Despite this distinctive biochemical characteristic, few studies have explored the biological role of phosphonolipids, although a protective function has been inferred based on chemical and biological stability. We analyzed two species of marine mollusks, the blue mussel Mytilus edulis and pacific oyster Crassostrea gigas , and determined the diversity of phosphonolipids and their distribution in different organs. High-resolution spatial metabolomics revealed that the lipidome varies significantly between tissues within one organ. Despite their chemical similarity, we observed a high heterogeneity of phosphonolipid distributions that originated from minor structural differences. Some phosphonolipids are ubiquitously distributed, while others are present almost exclusively in the layer of ciliated epithelial cells. This distinct localization of certain phosphonolipids in tissues exposed to the environment could support the hypothesis of a protective function in mollusks. This study highlights that the tissue specific distribution of an individual metabolite can be a valuable tool for inferring its function and guiding functional analyses. |
author2 |
The Max Planck Society The MARUM Cluster of Excellence ‘The Ocean Floor’ (Deutsche Forschungsgemeinschaft European Research Council Advanced Grant |
format |
Article in Journal/Newspaper |
author |
Bourceau, Patric Michellod, Dolma Geier, Benedikt Liebeke, Manuel |
spellingShingle |
Bourceau, Patric Michellod, Dolma Geier, Benedikt Liebeke, Manuel Spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves |
author_facet |
Bourceau, Patric Michellod, Dolma Geier, Benedikt Liebeke, Manuel |
author_sort |
Bourceau, Patric |
title |
Spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves |
title_short |
Spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves |
title_full |
Spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves |
title_fullStr |
Spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves |
title_full_unstemmed |
Spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves |
title_sort |
spatial metabolomics shows contrasting phosphonolipid distributions in tissues of marine bivalves |
publisher |
PeerJ |
publishDate |
2022 |
url |
http://dx.doi.org/10.7717/peerj-achem.21 https://peerj.com/articles/achem-21.pdf https://peerj.com/articles/achem-21.xml https://peerj.com/articles/achem-21.html |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
PeerJ Analytical Chemistry volume 4, page e21 ISSN 2691-6630 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.7717/peerj-achem.21 |
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PeerJ Analytical Chemistry |
container_volume |
4 |
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e21 |
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1810440746180804608 |