Identification of the Major Sex-Determining Region of Turbot ( Scophthalmus maximus)
Abstract Sex determination in fish is a labile character in evolutionary terms. The sex-determining (SD) master gene can differ even between closely related fish species. This group is an interesting model for studying the evolution of the SD region and the gonadal differentiation pathway. The turbo...
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Language: | English |
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Oxford University Press (OUP)
2009
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Online Access: | http://dx.doi.org/10.1534/genetics.109.107979 https://academic.oup.com/genetics/article-pdf/183/4/1443/46850208/genetics1443.pdf |
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croxfordunivpr:10.1534/genetics.109.107979 2024-06-23T07:56:36+00:00 Identification of the Major Sex-Determining Region of Turbot ( Scophthalmus maximus) Martínez, Paulino Bouza, Carmen Hermida, Miguel Fernández, Jesús Toro, Miguel Angel Vera, Manuel Pardo, Belén Millán, Adrián Fernández, Carlos Vilas, Román Viñas, Ana Sánchez, Laura Felip, Alicia Piferrer, Francesc Ferreiro, Isabel Cabaleiro, Santiago 2009 http://dx.doi.org/10.1534/genetics.109.107979 https://academic.oup.com/genetics/article-pdf/183/4/1443/46850208/genetics1443.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Genetics volume 183, issue 4, page 1443-1452 ISSN 1943-2631 journal-article 2009 croxfordunivpr https://doi.org/10.1534/genetics.109.107979 2024-06-11T04:20:44Z Abstract Sex determination in fish is a labile character in evolutionary terms. The sex-determining (SD) master gene can differ even between closely related fish species. This group is an interesting model for studying the evolution of the SD region and the gonadal differentiation pathway. The turbot (Scophthalmus maximus) is a flatfish of great commercial value, where a strong sexual dimorphism exists for growth rate. Following a QTL and marker association approach in five families and a natural population, we identified the main SD region of turbot at the proximal end of linkage group (LG) 5, close to the SmaUSC-E30 marker. The refined map of this region suggested that this marker would be 2.6 cM and 1.4 Mb from the putative SD gene. This region appeared mostly undifferentiated between males and females, and no relevant recombination frequency differences were detected between sexes. Comparative genomics of LG5 marker sequences against five model species showed no similarity of this chromosome to the sex chromosomes of medaka, stickleback, and fugu, but suggested a similarity to a sex-associated QTL from Oreochromis spp. The segregation analysis of the closest markers to the SD region demonstrated a ZW/ZZ model of sex determination in turbot. A small proportion of families did not fit perfectly with this model, which suggests that other minor genetic and/or environmental factors are involved in sex determination in this species. Article in Journal/Newspaper Scophthalmus maximus Turbot Oxford University Press Genetics 183 4 1443 1452 |
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Oxford University Press |
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English |
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Abstract Sex determination in fish is a labile character in evolutionary terms. The sex-determining (SD) master gene can differ even between closely related fish species. This group is an interesting model for studying the evolution of the SD region and the gonadal differentiation pathway. The turbot (Scophthalmus maximus) is a flatfish of great commercial value, where a strong sexual dimorphism exists for growth rate. Following a QTL and marker association approach in five families and a natural population, we identified the main SD region of turbot at the proximal end of linkage group (LG) 5, close to the SmaUSC-E30 marker. The refined map of this region suggested that this marker would be 2.6 cM and 1.4 Mb from the putative SD gene. This region appeared mostly undifferentiated between males and females, and no relevant recombination frequency differences were detected between sexes. Comparative genomics of LG5 marker sequences against five model species showed no similarity of this chromosome to the sex chromosomes of medaka, stickleback, and fugu, but suggested a similarity to a sex-associated QTL from Oreochromis spp. The segregation analysis of the closest markers to the SD region demonstrated a ZW/ZZ model of sex determination in turbot. A small proportion of families did not fit perfectly with this model, which suggests that other minor genetic and/or environmental factors are involved in sex determination in this species. |
format |
Article in Journal/Newspaper |
author |
Martínez, Paulino Bouza, Carmen Hermida, Miguel Fernández, Jesús Toro, Miguel Angel Vera, Manuel Pardo, Belén Millán, Adrián Fernández, Carlos Vilas, Román Viñas, Ana Sánchez, Laura Felip, Alicia Piferrer, Francesc Ferreiro, Isabel Cabaleiro, Santiago |
spellingShingle |
Martínez, Paulino Bouza, Carmen Hermida, Miguel Fernández, Jesús Toro, Miguel Angel Vera, Manuel Pardo, Belén Millán, Adrián Fernández, Carlos Vilas, Román Viñas, Ana Sánchez, Laura Felip, Alicia Piferrer, Francesc Ferreiro, Isabel Cabaleiro, Santiago Identification of the Major Sex-Determining Region of Turbot ( Scophthalmus maximus) |
author_facet |
Martínez, Paulino Bouza, Carmen Hermida, Miguel Fernández, Jesús Toro, Miguel Angel Vera, Manuel Pardo, Belén Millán, Adrián Fernández, Carlos Vilas, Román Viñas, Ana Sánchez, Laura Felip, Alicia Piferrer, Francesc Ferreiro, Isabel Cabaleiro, Santiago |
author_sort |
Martínez, Paulino |
title |
Identification of the Major Sex-Determining Region of Turbot ( Scophthalmus maximus) |
title_short |
Identification of the Major Sex-Determining Region of Turbot ( Scophthalmus maximus) |
title_full |
Identification of the Major Sex-Determining Region of Turbot ( Scophthalmus maximus) |
title_fullStr |
Identification of the Major Sex-Determining Region of Turbot ( Scophthalmus maximus) |
title_full_unstemmed |
Identification of the Major Sex-Determining Region of Turbot ( Scophthalmus maximus) |
title_sort |
identification of the major sex-determining region of turbot ( scophthalmus maximus) |
publisher |
Oxford University Press (OUP) |
publishDate |
2009 |
url |
http://dx.doi.org/10.1534/genetics.109.107979 https://academic.oup.com/genetics/article-pdf/183/4/1443/46850208/genetics1443.pdf |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Genetics volume 183, issue 4, page 1443-1452 ISSN 1943-2631 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1534/genetics.109.107979 |
container_title |
Genetics |
container_volume |
183 |
container_issue |
4 |
container_start_page |
1443 |
op_container_end_page |
1452 |
_version_ |
1802649862186991616 |