De Novo Assembly of a High-Quality Reference Genome for the Horned Lark ( Eremophila alpestris )
Abstract The Horned Lark (Eremophila alpestris) is a small songbird that exhibits remarkable geographic variation in appearance and habitat across an expansive distribution. While E. alpestris has been the focus of many ecological and evolutionary studies, we still lack a highly contiguous genome as...
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croxfordunivpr:10.1534/g3.119.400846 2024-04-28T08:17:57+00:00 De Novo Assembly of a High-Quality Reference Genome for the Horned Lark ( Eremophila alpestris ) Mason, Nicholas A Pulgarin, Paulo Cadena, Carlos Daniel Lovette, Irby J 2020 http://dx.doi.org/10.1534/g3.119.400846 http://academic.oup.com/g3journal/article-pdf/10/2/475/37180639/g3journal0475.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model G3 Genes|Genomes|Genetics volume 10, issue 2, page 475-478 ISSN 2160-1836 Genetics (clinical) Genetics Molecular Biology journal-article 2020 croxfordunivpr https://doi.org/10.1534/g3.119.400846 2024-04-09T07:55:26Z Abstract The Horned Lark (Eremophila alpestris) is a small songbird that exhibits remarkable geographic variation in appearance and habitat across an expansive distribution. While E. alpestris has been the focus of many ecological and evolutionary studies, we still lack a highly contiguous genome assembly for the Horned Lark and related taxa (Alaudidae). Here, we present CLO_EAlp_1.0, a highly contiguous assembly for E. alpestris generated from a blood sample of a wild, male bird captured in the Altiplano Cundiboyacense of Colombia. By combining short-insert and mate-pair libraries with the ALLPATHS-LG genome assembly pipeline, we generated a 1.04 Gb assembly comprised of 2713 scaffolds, with a largest scaffold size of 31.81 Mb, a scaffold N50 of 9.42 Mb, and a scaffold L50 of 30. These scaffolds were assembled from 23685 contigs, with a largest contig size of 1.69 Mb, a contig N50 of 193.81 kb, and a contig L50 of 1429. Our assembly pipeline also produced a single mitochondrial DNA contig of 14.00 kb. After polishing the genome, we identified 94.5% of single-copy gene orthologs from an Aves data set and 97.7% of single-copy gene orthologs from a vertebrata data set, which further demonstrates the high quality of our assembly. We anticipate that this genomic resource will be useful to the broader ornithological community and those interested in studying the evolutionary history and ecological interactions of larks, which comprise a widespread, yet understudied lineage of songbirds. Article in Journal/Newspaper Eremophila alpestris Oxford University Press G3 Genes|Genomes|Genetics 10 2 475 478 |
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Oxford University Press |
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language |
English |
topic |
Genetics (clinical) Genetics Molecular Biology |
spellingShingle |
Genetics (clinical) Genetics Molecular Biology Mason, Nicholas A Pulgarin, Paulo Cadena, Carlos Daniel Lovette, Irby J De Novo Assembly of a High-Quality Reference Genome for the Horned Lark ( Eremophila alpestris ) |
topic_facet |
Genetics (clinical) Genetics Molecular Biology |
description |
Abstract The Horned Lark (Eremophila alpestris) is a small songbird that exhibits remarkable geographic variation in appearance and habitat across an expansive distribution. While E. alpestris has been the focus of many ecological and evolutionary studies, we still lack a highly contiguous genome assembly for the Horned Lark and related taxa (Alaudidae). Here, we present CLO_EAlp_1.0, a highly contiguous assembly for E. alpestris generated from a blood sample of a wild, male bird captured in the Altiplano Cundiboyacense of Colombia. By combining short-insert and mate-pair libraries with the ALLPATHS-LG genome assembly pipeline, we generated a 1.04 Gb assembly comprised of 2713 scaffolds, with a largest scaffold size of 31.81 Mb, a scaffold N50 of 9.42 Mb, and a scaffold L50 of 30. These scaffolds were assembled from 23685 contigs, with a largest contig size of 1.69 Mb, a contig N50 of 193.81 kb, and a contig L50 of 1429. Our assembly pipeline also produced a single mitochondrial DNA contig of 14.00 kb. After polishing the genome, we identified 94.5% of single-copy gene orthologs from an Aves data set and 97.7% of single-copy gene orthologs from a vertebrata data set, which further demonstrates the high quality of our assembly. We anticipate that this genomic resource will be useful to the broader ornithological community and those interested in studying the evolutionary history and ecological interactions of larks, which comprise a widespread, yet understudied lineage of songbirds. |
format |
Article in Journal/Newspaper |
author |
Mason, Nicholas A Pulgarin, Paulo Cadena, Carlos Daniel Lovette, Irby J |
author_facet |
Mason, Nicholas A Pulgarin, Paulo Cadena, Carlos Daniel Lovette, Irby J |
author_sort |
Mason, Nicholas A |
title |
De Novo Assembly of a High-Quality Reference Genome for the Horned Lark ( Eremophila alpestris ) |
title_short |
De Novo Assembly of a High-Quality Reference Genome for the Horned Lark ( Eremophila alpestris ) |
title_full |
De Novo Assembly of a High-Quality Reference Genome for the Horned Lark ( Eremophila alpestris ) |
title_fullStr |
De Novo Assembly of a High-Quality Reference Genome for the Horned Lark ( Eremophila alpestris ) |
title_full_unstemmed |
De Novo Assembly of a High-Quality Reference Genome for the Horned Lark ( Eremophila alpestris ) |
title_sort |
de novo assembly of a high-quality reference genome for the horned lark ( eremophila alpestris ) |
publisher |
Oxford University Press (OUP) |
publishDate |
2020 |
url |
http://dx.doi.org/10.1534/g3.119.400846 http://academic.oup.com/g3journal/article-pdf/10/2/475/37180639/g3journal0475.pdf |
genre |
Eremophila alpestris |
genre_facet |
Eremophila alpestris |
op_source |
G3 Genes|Genomes|Genetics volume 10, issue 2, page 475-478 ISSN 2160-1836 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1534/g3.119.400846 |
container_title |
G3 Genes|Genomes|Genetics |
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10 |
container_issue |
2 |
container_start_page |
475 |
op_container_end_page |
478 |
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1797582205680091136 |