The Chromosome-Level Genome Assembly of European Grayling Reveals Aspects of a Unique Genome Evolution Process Within Salmonids
Abstract Salmonids represent an intriguing taxonomical group for investigating genome evolution in vertebrates due to their relatively recent last common whole genome duplication event, which occurred between 80 and 100 million years ago. Here, we report on the chromosome-level genome assembly of Eu...
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Online Access: | http://dx.doi.org/10.1534/g3.118.200919 http://academic.oup.com/g3journal/article-pdf/9/5/1283/37178781/g3journal1283.pdf |
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croxfordunivpr:10.1534/g3.118.200919 2024-05-19T07:37:26+00:00 The Chromosome-Level Genome Assembly of European Grayling Reveals Aspects of a Unique Genome Evolution Process Within Salmonids Sävilammi, Tiina Primmer, Craig R Varadharajan, Srinidhi Guyomard, René Guiguen, Yann Sandve, Simen R Vøllestad, L Asbjørn Papakostas, Spiros Lien, Sigbjørn 2019 http://dx.doi.org/10.1534/g3.118.200919 http://academic.oup.com/g3journal/article-pdf/9/5/1283/37178781/g3journal1283.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model G3 Genes|Genomes|Genetics volume 9, issue 5, page 1283-1294 ISSN 2160-1836 journal-article 2019 croxfordunivpr https://doi.org/10.1534/g3.118.200919 2024-05-02T09:31:02Z Abstract Salmonids represent an intriguing taxonomical group for investigating genome evolution in vertebrates due to their relatively recent last common whole genome duplication event, which occurred between 80 and 100 million years ago. Here, we report on the chromosome-level genome assembly of European grayling (Thymallus thymallus), which represents one of the earliest diverged salmonid subfamilies. To achieve this, we first generated relatively long genomic scaffolds by using a previously published draft genome assembly along with long-read sequencing data and a linkage map. We then merged those scaffolds by applying synteny evidence from the Atlantic salmon (Salmo salar) genome. Comparisons of the European grayling genome assembly to the genomes of Atlantic salmon and Northern pike (Esox lucius), the latter used as a nonduplicated outgroup, detailed aspects of the characteristic chromosome evolution process that has taken place in European grayling. While Atlantic salmon and other salmonid genomes are portrayed by the typical occurrence of numerous chromosomal fusions, European grayling chromosomes were confirmed to be fusion-free and were characterized by a relatively large proportion of paracentric and pericentric inversions. We further reported on transposable elements specific to either the European grayling or Atlantic salmon genome, on the male-specific sdY gene in the European grayling chromosome 11A, and on regions under residual tetrasomy in the homeologous European grayling chromosome pairs 9A-9B and 25A-25B. The same chromosome pairs have been observed under residual tetrasomy in Atlantic salmon and in other salmonids, suggesting that this feature has been conserved since the subfamily split. Article in Journal/Newspaper Atlantic salmon Salmo salar Oxford University Press G3 Genes|Genomes|Genetics 9 5 1283 1294 |
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English |
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Abstract Salmonids represent an intriguing taxonomical group for investigating genome evolution in vertebrates due to their relatively recent last common whole genome duplication event, which occurred between 80 and 100 million years ago. Here, we report on the chromosome-level genome assembly of European grayling (Thymallus thymallus), which represents one of the earliest diverged salmonid subfamilies. To achieve this, we first generated relatively long genomic scaffolds by using a previously published draft genome assembly along with long-read sequencing data and a linkage map. We then merged those scaffolds by applying synteny evidence from the Atlantic salmon (Salmo salar) genome. Comparisons of the European grayling genome assembly to the genomes of Atlantic salmon and Northern pike (Esox lucius), the latter used as a nonduplicated outgroup, detailed aspects of the characteristic chromosome evolution process that has taken place in European grayling. While Atlantic salmon and other salmonid genomes are portrayed by the typical occurrence of numerous chromosomal fusions, European grayling chromosomes were confirmed to be fusion-free and were characterized by a relatively large proportion of paracentric and pericentric inversions. We further reported on transposable elements specific to either the European grayling or Atlantic salmon genome, on the male-specific sdY gene in the European grayling chromosome 11A, and on regions under residual tetrasomy in the homeologous European grayling chromosome pairs 9A-9B and 25A-25B. The same chromosome pairs have been observed under residual tetrasomy in Atlantic salmon and in other salmonids, suggesting that this feature has been conserved since the subfamily split. |
format |
Article in Journal/Newspaper |
author |
Sävilammi, Tiina Primmer, Craig R Varadharajan, Srinidhi Guyomard, René Guiguen, Yann Sandve, Simen R Vøllestad, L Asbjørn Papakostas, Spiros Lien, Sigbjørn |
spellingShingle |
Sävilammi, Tiina Primmer, Craig R Varadharajan, Srinidhi Guyomard, René Guiguen, Yann Sandve, Simen R Vøllestad, L Asbjørn Papakostas, Spiros Lien, Sigbjørn The Chromosome-Level Genome Assembly of European Grayling Reveals Aspects of a Unique Genome Evolution Process Within Salmonids |
author_facet |
Sävilammi, Tiina Primmer, Craig R Varadharajan, Srinidhi Guyomard, René Guiguen, Yann Sandve, Simen R Vøllestad, L Asbjørn Papakostas, Spiros Lien, Sigbjørn |
author_sort |
Sävilammi, Tiina |
title |
The Chromosome-Level Genome Assembly of European Grayling Reveals Aspects of a Unique Genome Evolution Process Within Salmonids |
title_short |
The Chromosome-Level Genome Assembly of European Grayling Reveals Aspects of a Unique Genome Evolution Process Within Salmonids |
title_full |
The Chromosome-Level Genome Assembly of European Grayling Reveals Aspects of a Unique Genome Evolution Process Within Salmonids |
title_fullStr |
The Chromosome-Level Genome Assembly of European Grayling Reveals Aspects of a Unique Genome Evolution Process Within Salmonids |
title_full_unstemmed |
The Chromosome-Level Genome Assembly of European Grayling Reveals Aspects of a Unique Genome Evolution Process Within Salmonids |
title_sort |
chromosome-level genome assembly of european grayling reveals aspects of a unique genome evolution process within salmonids |
publisher |
Oxford University Press (OUP) |
publishDate |
2019 |
url |
http://dx.doi.org/10.1534/g3.118.200919 http://academic.oup.com/g3journal/article-pdf/9/5/1283/37178781/g3journal1283.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
G3 Genes|Genomes|Genetics volume 9, issue 5, page 1283-1294 ISSN 2160-1836 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1534/g3.118.200919 |
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G3 Genes|Genomes|Genetics |
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9 |
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5 |
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1283 |
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1294 |
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1799476719463170048 |