Analysis of CRISPR–Cas system and antimicrobial resistance in Staphylococcus coagulans isolates
Abstract CRISPR–Cas system contributes adaptive immunity to protect the bacterial and archaeal genome against invading mobile genetic elements. In this study, an attempt was made to characterize the CRISPR–Cas system in Staphylococcus coagulans, the second most prevalent coagulase positive staphyloc...
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croxfordunivpr:10.1111/lam.13713 2024-05-12T07:55:10+00:00 Analysis of CRISPR–Cas system and antimicrobial resistance in Staphylococcus coagulans isolates Murugesan, A.C. Varughese, H.S. Department of Science and Technology-Science and Engineering Research Board, Government of India 2022 http://dx.doi.org/10.1111/lam.13713 https://onlinelibrary.wiley.com/doi/pdf/10.1111/lam.13713 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/lam.13713 https://academic.oup.com/lambio/article-pdf/75/1/126/48737714/lambio0126.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Letters in Applied Microbiology volume 75, issue 1, page 126-134 ISSN 1472-765X 0266-8254 Applied Microbiology and Biotechnology journal-article 2022 croxfordunivpr https://doi.org/10.1111/lam.13713 2024-04-18T08:14:47Z Abstract CRISPR–Cas system contributes adaptive immunity to protect the bacterial and archaeal genome against invading mobile genetic elements. In this study, an attempt was made to characterize the CRISPR–Cas system in Staphylococcus coagulans, the second most prevalent coagulase positive staphylococci causing skin infections in dogs. Out of 45 S. coagulans isolates, 42/45 (93·33%) strains contained CRISPR–Cas system and 45 confirmed CRISPR system was identified in 42 S. coagulans isolates. The length of CRISPR loci ranged from 167 to 2477 bp, and the number of spacers in each CRISPR was varied from two spacers to as high as 37 numbers. Direct repeat (DR) sequences were between 30 and 37, but most (35/45) of the DRs contained 36 sequences. The predominant S. coagulans strains 29/45 did not possess any antimicrobial resistant genes (ARG); 26/29 strains contained Type IIC CRISPR–Cas system. Three isolates from Antarctica seals neither contain CRISPR–Cas system nor ARG. Only 15/45 S. coagulans strains (33·33%) harboured at least one ARG and 13/15 of them were having mecA gene. All the methicillin susceptible S. coagulans isolates contained Type IIC CRISPR–Cas system. In contrast, many (10/13) S. coagulans isolates which were methicillin resistant had Type IIIA CRISPR–Cas system, and this Type IIIA CRISPR–Cas system was present within the SCCmec mobile genetic element. Hence, this study suggests that Type II CRISPR–Cas in S. coagulans isolates might have played a possible role in preventing acquisition of plasmid/phage invasion and Type IIIA CRISPR–Cas system may have an insignificant role in the prevention of horizontal gene transfer of antimicrobial resistance genes in S. coagulans species. Article in Journal/Newspaper Antarc* Antarctica Oxford University Press Letters in Applied Microbiology 75 1 126 134 |
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English |
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Applied Microbiology and Biotechnology |
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Applied Microbiology and Biotechnology Murugesan, A.C. Varughese, H.S. Analysis of CRISPR–Cas system and antimicrobial resistance in Staphylococcus coagulans isolates |
topic_facet |
Applied Microbiology and Biotechnology |
description |
Abstract CRISPR–Cas system contributes adaptive immunity to protect the bacterial and archaeal genome against invading mobile genetic elements. In this study, an attempt was made to characterize the CRISPR–Cas system in Staphylococcus coagulans, the second most prevalent coagulase positive staphylococci causing skin infections in dogs. Out of 45 S. coagulans isolates, 42/45 (93·33%) strains contained CRISPR–Cas system and 45 confirmed CRISPR system was identified in 42 S. coagulans isolates. The length of CRISPR loci ranged from 167 to 2477 bp, and the number of spacers in each CRISPR was varied from two spacers to as high as 37 numbers. Direct repeat (DR) sequences were between 30 and 37, but most (35/45) of the DRs contained 36 sequences. The predominant S. coagulans strains 29/45 did not possess any antimicrobial resistant genes (ARG); 26/29 strains contained Type IIC CRISPR–Cas system. Three isolates from Antarctica seals neither contain CRISPR–Cas system nor ARG. Only 15/45 S. coagulans strains (33·33%) harboured at least one ARG and 13/15 of them were having mecA gene. All the methicillin susceptible S. coagulans isolates contained Type IIC CRISPR–Cas system. In contrast, many (10/13) S. coagulans isolates which were methicillin resistant had Type IIIA CRISPR–Cas system, and this Type IIIA CRISPR–Cas system was present within the SCCmec mobile genetic element. Hence, this study suggests that Type II CRISPR–Cas in S. coagulans isolates might have played a possible role in preventing acquisition of plasmid/phage invasion and Type IIIA CRISPR–Cas system may have an insignificant role in the prevention of horizontal gene transfer of antimicrobial resistance genes in S. coagulans species. |
author2 |
Department of Science and Technology-Science and Engineering Research Board, Government of India |
format |
Article in Journal/Newspaper |
author |
Murugesan, A.C. Varughese, H.S. |
author_facet |
Murugesan, A.C. Varughese, H.S. |
author_sort |
Murugesan, A.C. |
title |
Analysis of CRISPR–Cas system and antimicrobial resistance in Staphylococcus coagulans isolates |
title_short |
Analysis of CRISPR–Cas system and antimicrobial resistance in Staphylococcus coagulans isolates |
title_full |
Analysis of CRISPR–Cas system and antimicrobial resistance in Staphylococcus coagulans isolates |
title_fullStr |
Analysis of CRISPR–Cas system and antimicrobial resistance in Staphylococcus coagulans isolates |
title_full_unstemmed |
Analysis of CRISPR–Cas system and antimicrobial resistance in Staphylococcus coagulans isolates |
title_sort |
analysis of crispr–cas system and antimicrobial resistance in staphylococcus coagulans isolates |
publisher |
Oxford University Press (OUP) |
publishDate |
2022 |
url |
http://dx.doi.org/10.1111/lam.13713 https://onlinelibrary.wiley.com/doi/pdf/10.1111/lam.13713 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/lam.13713 https://academic.oup.com/lambio/article-pdf/75/1/126/48737714/lambio0126.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Letters in Applied Microbiology volume 75, issue 1, page 126-134 ISSN 1472-765X 0266-8254 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1111/lam.13713 |
container_title |
Letters in Applied Microbiology |
container_volume |
75 |
container_issue |
1 |
container_start_page |
126 |
op_container_end_page |
134 |
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1798834641278337024 |