Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers
Abstract In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts...
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croxfordunivpr:10.1111/lam.12143 2024-09-15T17:47:55+00:00 Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers Geiger, M. Guitton, Y. Vansteelandt, M. Kerzaon, I. Blanchet, E. Robiou du Pont, T. Frisvad, J.C. Hess, P. Pouchus, Y.F. Grovel, O. 2013 http://dx.doi.org/10.1111/lam.12143 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Flam.12143 https://academic.oup.com/lambio/article-pdf/57/5/385/49002286/lambio0385.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Letters in Applied Microbiology volume 57, issue 5, page 385-392 ISSN 1472-765X 0266-8254 journal-article 2013 croxfordunivpr https://doi.org/10.1111/lam.12143 2024-07-15T04:23:57Z Abstract In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with other constituent identical to YES medium. When shellfish-derived strains of fungi were grown on MES medium, extracts were found to be more cytotoxic than on the YES medium for some of the strains. HPLC-UV/DAD-MS/MS dereplication of extracts from Penicillium marinum and P. restrictum strains grown on MES medium showed the enhancement of the production of some cytotoxic compounds. The mycotoxin patulin was detected in some P. antarcticum extracts, and its presence seemed to be related to their cytotoxicity. Thus, the enhancement of the toxicity of extracts obtained from shellfish-derived Penicillium strains grown on a host-derived medium, and the production of metabolites such as patulin suggests that a survey of mycotoxins in edible shellfish should be considered. Significance and Impact of the Study Penicillium strains isolated from bivalve molluscs produce extracts exhibiting a higher cytotoxicity than extracts from Penicillium strains isolated from the surrounding marine environment. The use of a mussel-based medium for cultures of some shellfish-derived strains enhances the cytotoxicity of extracts when compared with classical media. The production of cytotoxic compounds and of the mycotoxin patulin on such a host-derived medium highlights a ... Article in Journal/Newspaper Antarc* Oxford University Press Letters in Applied Microbiology 57 5 385 392 |
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Oxford University Press |
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croxfordunivpr |
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English |
description |
Abstract In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with other constituent identical to YES medium. When shellfish-derived strains of fungi were grown on MES medium, extracts were found to be more cytotoxic than on the YES medium for some of the strains. HPLC-UV/DAD-MS/MS dereplication of extracts from Penicillium marinum and P. restrictum strains grown on MES medium showed the enhancement of the production of some cytotoxic compounds. The mycotoxin patulin was detected in some P. antarcticum extracts, and its presence seemed to be related to their cytotoxicity. Thus, the enhancement of the toxicity of extracts obtained from shellfish-derived Penicillium strains grown on a host-derived medium, and the production of metabolites such as patulin suggests that a survey of mycotoxins in edible shellfish should be considered. Significance and Impact of the Study Penicillium strains isolated from bivalve molluscs produce extracts exhibiting a higher cytotoxicity than extracts from Penicillium strains isolated from the surrounding marine environment. The use of a mussel-based medium for cultures of some shellfish-derived strains enhances the cytotoxicity of extracts when compared with classical media. The production of cytotoxic compounds and of the mycotoxin patulin on such a host-derived medium highlights a ... |
format |
Article in Journal/Newspaper |
author |
Geiger, M. Guitton, Y. Vansteelandt, M. Kerzaon, I. Blanchet, E. Robiou du Pont, T. Frisvad, J.C. Hess, P. Pouchus, Y.F. Grovel, O. |
spellingShingle |
Geiger, M. Guitton, Y. Vansteelandt, M. Kerzaon, I. Blanchet, E. Robiou du Pont, T. Frisvad, J.C. Hess, P. Pouchus, Y.F. Grovel, O. Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
author_facet |
Geiger, M. Guitton, Y. Vansteelandt, M. Kerzaon, I. Blanchet, E. Robiou du Pont, T. Frisvad, J.C. Hess, P. Pouchus, Y.F. Grovel, O. |
author_sort |
Geiger, M. |
title |
Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
title_short |
Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
title_full |
Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
title_fullStr |
Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
title_full_unstemmed |
Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers |
title_sort |
cytotoxicity and mycotoxin production of shellfish-derived penicillium spp., a risk for shellfish consumers |
publisher |
Oxford University Press (OUP) |
publishDate |
2013 |
url |
http://dx.doi.org/10.1111/lam.12143 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Flam.12143 https://academic.oup.com/lambio/article-pdf/57/5/385/49002286/lambio0385.pdf |
genre |
Antarc* |
genre_facet |
Antarc* |
op_source |
Letters in Applied Microbiology volume 57, issue 5, page 385-392 ISSN 1472-765X 0266-8254 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1111/lam.12143 |
container_title |
Letters in Applied Microbiology |
container_volume |
57 |
container_issue |
5 |
container_start_page |
385 |
op_container_end_page |
392 |
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1810497627644493824 |