Silene tatarica microsatellites are frequently located in repetitive DNA
Abstract The genomic distribution of microsatellites can be explained by DNA slippage, slippage like processes and base substitutions. Nevertheless, microsatellites are also frequently associated with repetitive DNA, raising the question of the relative contributions of these processes to microsatel...
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croxfordunivpr:10.1111/j.1420-9101.2006.01118.x 2024-01-28T10:09:09+01:00 Silene tatarica microsatellites are frequently located in repetitive DNA Tero, N. Neumeier, H. Gudavalli, R. Schlötterer, C. 2006 http://dx.doi.org/10.1111/j.1420-9101.2006.01118.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1420-9101.2006.01118.x https://academic.oup.com/jeb/article-pdf/19/5/1612/54440432/jevbio1612.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Evolutionary Biology volume 19, issue 5, page 1612-1619 ISSN 1010-061X 1420-9101 Ecology, Evolution, Behavior and Systematics journal-article 2006 croxfordunivpr https://doi.org/10.1111/j.1420-9101.2006.01118.x 2023-12-29T09:47:15Z Abstract The genomic distribution of microsatellites can be explained by DNA slippage, slippage like processes and base substitutions. Nevertheless, microsatellites are also frequently associated with repetitive DNA, raising the question of the relative contributions of these processes to microsatellite genesis. We show that in Silene tatarica about 50% of the microsatellites isolated by an enrichment cloning protocol are associated with repetitive DNA. Based on the flanking sequences, we distinguished seven different classes of repetitive DNA. PCR primers designed for the flanking sequences of an individual clone amplified a heterogeneous family of repetitive DNA. Despite considerable variation in the flanking sequence (π = 0.108), the microsatellite repeats did not show any evidence for decay. Rather, we observed the emergence of a new repeat type that probably arose by mutation and was spread by replication slippage. In fact, a complete repeat type switch could be observed among the analysed clones. We propose that the analysis of microsatellite sequences embedded in repetitive DNA provides a hitherto largely unexplored tool to study microsatellite evolution. Article in Journal/Newspaper Silene tatarica Oxford University Press (via Crossref) Journal of Evolutionary Biology 19 5 1612 1619 |
institution |
Open Polar |
collection |
Oxford University Press (via Crossref) |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Ecology, Evolution, Behavior and Systematics Tero, N. Neumeier, H. Gudavalli, R. Schlötterer, C. Silene tatarica microsatellites are frequently located in repetitive DNA |
topic_facet |
Ecology, Evolution, Behavior and Systematics |
description |
Abstract The genomic distribution of microsatellites can be explained by DNA slippage, slippage like processes and base substitutions. Nevertheless, microsatellites are also frequently associated with repetitive DNA, raising the question of the relative contributions of these processes to microsatellite genesis. We show that in Silene tatarica about 50% of the microsatellites isolated by an enrichment cloning protocol are associated with repetitive DNA. Based on the flanking sequences, we distinguished seven different classes of repetitive DNA. PCR primers designed for the flanking sequences of an individual clone amplified a heterogeneous family of repetitive DNA. Despite considerable variation in the flanking sequence (π = 0.108), the microsatellite repeats did not show any evidence for decay. Rather, we observed the emergence of a new repeat type that probably arose by mutation and was spread by replication slippage. In fact, a complete repeat type switch could be observed among the analysed clones. We propose that the analysis of microsatellite sequences embedded in repetitive DNA provides a hitherto largely unexplored tool to study microsatellite evolution. |
format |
Article in Journal/Newspaper |
author |
Tero, N. Neumeier, H. Gudavalli, R. Schlötterer, C. |
author_facet |
Tero, N. Neumeier, H. Gudavalli, R. Schlötterer, C. |
author_sort |
Tero, N. |
title |
Silene tatarica microsatellites are frequently located in repetitive DNA |
title_short |
Silene tatarica microsatellites are frequently located in repetitive DNA |
title_full |
Silene tatarica microsatellites are frequently located in repetitive DNA |
title_fullStr |
Silene tatarica microsatellites are frequently located in repetitive DNA |
title_full_unstemmed |
Silene tatarica microsatellites are frequently located in repetitive DNA |
title_sort |
silene tatarica microsatellites are frequently located in repetitive dna |
publisher |
Oxford University Press (OUP) |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1420-9101.2006.01118.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1420-9101.2006.01118.x https://academic.oup.com/jeb/article-pdf/19/5/1612/54440432/jevbio1612.pdf |
genre |
Silene tatarica |
genre_facet |
Silene tatarica |
op_source |
Journal of Evolutionary Biology volume 19, issue 5, page 1612-1619 ISSN 1010-061X 1420-9101 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1420-9101.2006.01118.x |
container_title |
Journal of Evolutionary Biology |
container_volume |
19 |
container_issue |
5 |
container_start_page |
1612 |
op_container_end_page |
1619 |
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1789338782358568960 |