The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma
Abstract Changes in plasmalemma permeability caused by excessive Cu2+ levels were examined in cells of a freshwater alga (Nitella flexilis) using a conventional microelectrode voltage-clamp technique. A rapid Cu2+-induced increase of plasmalemma conductance starting from 5 [mu]M Cu2+ was shown. Cu2+...
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Oxford University Press (OUP)
1997
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Online Access: | http://dx.doi.org/10.1104/pp.114.4.1313 http://academic.oup.com/plphys/article-pdf/114/4/1313/37088264/plphys_v114_4_1313.pdf |
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croxfordunivpr:10.1104/pp.114.4.1313 2024-04-07T07:54:11+00:00 The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma Demidchik, V. Sokolik, A. Yurin, V. 1997 http://dx.doi.org/10.1104/pp.114.4.1313 http://academic.oup.com/plphys/article-pdf/114/4/1313/37088264/plphys_v114_4_1313.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Plant Physiology volume 114, issue 4, page 1313-1325 ISSN 1532-2548 0032-0889 Plant Science Genetics Physiology journal-article 1997 croxfordunivpr https://doi.org/10.1104/pp.114.4.1313 2024-03-08T03:09:27Z Abstract Changes in plasmalemma permeability caused by excessive Cu2+ levels were examined in cells of a freshwater alga (Nitella flexilis) using a conventional microelectrode voltage-clamp technique. A rapid Cu2+-induced increase of plasmalemma conductance starting from 5 [mu]M Cu2+ was shown. Cu2+-induced plasmalemma conductance (ClGm) was nonselective and potential-independent, resembling the conductance of nonselective ionic leakage of the plasmalemma. The K+ channel conductance was shown to be unaltered by Cu2+, and a decrease in plasmalemma Cl- channel conductance at Cu2+ concentrations above 5 [mu]M was found. The depression of Cl- channels and ClGm were time-, dosage-, and Ca2+-dependent processes, revealing a great similarity in all parameters, with Ca2+ causing the preventive effect by shifting the effective Cu2+ concentrations to higher levels. This phenomenon may be explained by the same Cu2+-modified target on the plasmalemma both for ClGm and Cl- channel depression. In addition, a reversible, inhibitory effect of Cu2+ (>10 [mu]M) on the light-stimulated H+-ATPase electrogenic pump in the plasmalemma was demonstrated. This effect was Ca2+- independent, which made it possible to distinguish it from ClGm. Therefore, the Cu2+-induced dramatic alterations in plant cell plasmalemma permeability are caused mainly by nonselective conductance increases and electrogenic pump inhibition. Article in Journal/Newspaper Nitella flexilis Oxford University Press Plant Physiology 114 4 1313 1325 |
institution |
Open Polar |
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Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Plant Science Genetics Physiology |
spellingShingle |
Plant Science Genetics Physiology Demidchik, V. Sokolik, A. Yurin, V. The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma |
topic_facet |
Plant Science Genetics Physiology |
description |
Abstract Changes in plasmalemma permeability caused by excessive Cu2+ levels were examined in cells of a freshwater alga (Nitella flexilis) using a conventional microelectrode voltage-clamp technique. A rapid Cu2+-induced increase of plasmalemma conductance starting from 5 [mu]M Cu2+ was shown. Cu2+-induced plasmalemma conductance (ClGm) was nonselective and potential-independent, resembling the conductance of nonselective ionic leakage of the plasmalemma. The K+ channel conductance was shown to be unaltered by Cu2+, and a decrease in plasmalemma Cl- channel conductance at Cu2+ concentrations above 5 [mu]M was found. The depression of Cl- channels and ClGm were time-, dosage-, and Ca2+-dependent processes, revealing a great similarity in all parameters, with Ca2+ causing the preventive effect by shifting the effective Cu2+ concentrations to higher levels. This phenomenon may be explained by the same Cu2+-modified target on the plasmalemma both for ClGm and Cl- channel depression. In addition, a reversible, inhibitory effect of Cu2+ (>10 [mu]M) on the light-stimulated H+-ATPase electrogenic pump in the plasmalemma was demonstrated. This effect was Ca2+- independent, which made it possible to distinguish it from ClGm. Therefore, the Cu2+-induced dramatic alterations in plant cell plasmalemma permeability are caused mainly by nonselective conductance increases and electrogenic pump inhibition. |
format |
Article in Journal/Newspaper |
author |
Demidchik, V. Sokolik, A. Yurin, V. |
author_facet |
Demidchik, V. Sokolik, A. Yurin, V. |
author_sort |
Demidchik, V. |
title |
The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma |
title_short |
The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma |
title_full |
The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma |
title_fullStr |
The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma |
title_full_unstemmed |
The Effect of Cu2+ on Ion Transport Systems of the Plant Cell Plasmalemma |
title_sort |
effect of cu2+ on ion transport systems of the plant cell plasmalemma |
publisher |
Oxford University Press (OUP) |
publishDate |
1997 |
url |
http://dx.doi.org/10.1104/pp.114.4.1313 http://academic.oup.com/plphys/article-pdf/114/4/1313/37088264/plphys_v114_4_1313.pdf |
genre |
Nitella flexilis |
genre_facet |
Nitella flexilis |
op_source |
Plant Physiology volume 114, issue 4, page 1313-1325 ISSN 1532-2548 0032-0889 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1104/pp.114.4.1313 |
container_title |
Plant Physiology |
container_volume |
114 |
container_issue |
4 |
container_start_page |
1313 |
op_container_end_page |
1325 |
_version_ |
1795670564132618240 |