Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway
Abstract Dimethylsulfoniopropionate (DMSP) plays important roles in oceanic carbon and sulfur cycling and may significantly impact climate. It is a biomolecule synthesized from the methionine (Met) pathway and proposed to serve various physiological functions to aid in environmental stress adaptatio...
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croxfordunivpr:10.1104/pp.111.185025 2024-05-12T08:10:52+00:00 Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway Lyon, Barbara R. Lee, Peter A. Bennett, Jennifer M. DiTullio, Giacomo R. Janech, Michael G. 2011 http://dx.doi.org/10.1104/pp.111.185025 http://academic.oup.com/plphys/article-pdf/157/4/1926/37147603/plphys_v157_4_1926.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ Plant Physiology volume 157, issue 4, page 1926-1941 ISSN 1532-2548 Plant Science Genetics Physiology journal-article 2011 croxfordunivpr https://doi.org/10.1104/pp.111.185025 2024-04-18T08:16:00Z Abstract Dimethylsulfoniopropionate (DMSP) plays important roles in oceanic carbon and sulfur cycling and may significantly impact climate. It is a biomolecule synthesized from the methionine (Met) pathway and proposed to serve various physiological functions to aid in environmental stress adaptation through its compatible solute, cryoprotectant, and antioxidant properties. Yet, the enzymes and mechanisms regulating DMSP production are poorly understood. This study utilized a proteomics approach to investigate protein changes associated with salinity-induced DMSP increases in the model sea-ice diatom Fragilariopsis cylindrus (CCMP 1102). We hypothesized proteins associated with the Met-DMSP biosynthesis pathway would increase in relative abundance when challenged with elevated salinity. To test this hypothesis axenic log-phase cultures initially grown at a salinity of 35 were gradually shifted to a final salinity of 70 over a 24-h period. Intracellular DMSP was measured and two-dimensional gel electrophoresis was used to identify protein changes at 48 h after the shift. Intracellular DMSP increased by approximately 85% in the hypersaline cultures. One-third of the proteins increased under high salinity were associated with amino acid pathways. Three protein isoforms of S-adenosylhomo-cysteine hydrolase, which synthesizes a Met precursor, increased 1.8- to 2.1-fold, two isoforms of S-adenosyl Met synthetase increased 1.9- to 2.5-fold, and S-adenosyl Met methyltransferase increased by 2.8-fold, suggesting active methyl cycle proteins are recruited in the synthesis of DMSP. Proteins from the four enzyme classes of the proposed algal Met transaminase DMSP pathway were among the elevated proteins, supporting our hypothesis and providing candidate genes for future characterization studies. Article in Journal/Newspaper Sea ice Oxford University Press Plant Physiology 157 4 1926 1941 |
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English |
topic |
Plant Science Genetics Physiology |
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Plant Science Genetics Physiology Lyon, Barbara R. Lee, Peter A. Bennett, Jennifer M. DiTullio, Giacomo R. Janech, Michael G. Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway |
topic_facet |
Plant Science Genetics Physiology |
description |
Abstract Dimethylsulfoniopropionate (DMSP) plays important roles in oceanic carbon and sulfur cycling and may significantly impact climate. It is a biomolecule synthesized from the methionine (Met) pathway and proposed to serve various physiological functions to aid in environmental stress adaptation through its compatible solute, cryoprotectant, and antioxidant properties. Yet, the enzymes and mechanisms regulating DMSP production are poorly understood. This study utilized a proteomics approach to investigate protein changes associated with salinity-induced DMSP increases in the model sea-ice diatom Fragilariopsis cylindrus (CCMP 1102). We hypothesized proteins associated with the Met-DMSP biosynthesis pathway would increase in relative abundance when challenged with elevated salinity. To test this hypothesis axenic log-phase cultures initially grown at a salinity of 35 were gradually shifted to a final salinity of 70 over a 24-h period. Intracellular DMSP was measured and two-dimensional gel electrophoresis was used to identify protein changes at 48 h after the shift. Intracellular DMSP increased by approximately 85% in the hypersaline cultures. One-third of the proteins increased under high salinity were associated with amino acid pathways. Three protein isoforms of S-adenosylhomo-cysteine hydrolase, which synthesizes a Met precursor, increased 1.8- to 2.1-fold, two isoforms of S-adenosyl Met synthetase increased 1.9- to 2.5-fold, and S-adenosyl Met methyltransferase increased by 2.8-fold, suggesting active methyl cycle proteins are recruited in the synthesis of DMSP. Proteins from the four enzyme classes of the proposed algal Met transaminase DMSP pathway were among the elevated proteins, supporting our hypothesis and providing candidate genes for future characterization studies. |
format |
Article in Journal/Newspaper |
author |
Lyon, Barbara R. Lee, Peter A. Bennett, Jennifer M. DiTullio, Giacomo R. Janech, Michael G. |
author_facet |
Lyon, Barbara R. Lee, Peter A. Bennett, Jennifer M. DiTullio, Giacomo R. Janech, Michael G. |
author_sort |
Lyon, Barbara R. |
title |
Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway |
title_short |
Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway |
title_full |
Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway |
title_fullStr |
Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway |
title_full_unstemmed |
Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway |
title_sort |
proteomic analysis of a sea-ice diatom: salinity acclimation provides new insight into the dimethylsulfoniopropionate production pathway |
publisher |
Oxford University Press (OUP) |
publishDate |
2011 |
url |
http://dx.doi.org/10.1104/pp.111.185025 http://academic.oup.com/plphys/article-pdf/157/4/1926/37147603/plphys_v157_4_1926.pdf |
genre |
Sea ice |
genre_facet |
Sea ice |
op_source |
Plant Physiology volume 157, issue 4, page 1926-1941 ISSN 1532-2548 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1104/pp.111.185025 |
container_title |
Plant Physiology |
container_volume |
157 |
container_issue |
4 |
container_start_page |
1926 |
op_container_end_page |
1941 |
_version_ |
1798854412433620992 |