Genome-wide support for incipient Tula hantavirus species within a single rodent host lineage
Abstract Evolutionary divergence of viruses is most commonly driven by co-divergence with their hosts or through isolation of transmission after host shifts. It remains mostly unknown, however, whether divergent phylogenetic clades within named virus species represent functionally equivalent byprodu...
Published in: | Virus Evolution |
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Oxford University Press (OUP)
2024
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Online Access: | http://dx.doi.org/10.1093/ve/veae002 https://academic.oup.com/ve/advance-article-pdf/doi/10.1093/ve/veae002/55120087/veae002.pdf https://academic.oup.com/ve/article-pdf/10/1/veae002/56487491/veae002.pdf |
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croxfordunivpr:10.1093/ve/veae002 2024-03-03T08:43:42+00:00 Genome-wide support for incipient Tula hantavirus species within a single rodent host lineage Labutin, Anton Heckel, Gerald Swiss National Science Foundation Swiss National Science Foundation 2024 http://dx.doi.org/10.1093/ve/veae002 https://academic.oup.com/ve/advance-article-pdf/doi/10.1093/ve/veae002/55120087/veae002.pdf https://academic.oup.com/ve/article-pdf/10/1/veae002/56487491/veae002.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ Virus Evolution volume 10, issue 1 ISSN 2057-1577 Virology Microbiology journal-article 2024 croxfordunivpr https://doi.org/10.1093/ve/veae002 2024-02-05T10:32:10Z Abstract Evolutionary divergence of viruses is most commonly driven by co-divergence with their hosts or through isolation of transmission after host shifts. It remains mostly unknown, however, whether divergent phylogenetic clades within named virus species represent functionally equivalent byproducts of high evolutionary rates or rather incipient virus species. Here, we test these alternatives with genomic data from two widespread phylogenetic clades in Tula orthohantavirus (TULV) within a single evolutionary lineage of their natural rodent host, the common vole Microtus arvalis. We examined voles from forty-two locations in the contact region between clades for TULV infection by reverse transcription (RT)-PCR. Sequencing yielded twenty-three TULV Central North and twenty-one TULV Central South genomes, which differed by 14.9–18.5 per cent at the nucleotide and 2.2–3.7 per cent at the amino acid (AA) level without evidence of recombination or reassortment between clades. Geographic cline analyses demonstrated an abrupt (<1 km wide) transition between the parapatric TULV clades in continuous landscape. This transition was located within the Central mitochondrial lineage of M. arvalis, and genomic single nucleotide polymorphisms showed gradual mixing of host populations across it. Genomic differentiation of hosts was much weaker across the TULV Central North to South transition than across the nearby hybrid zone between two evolutionary lineages in the host. We suggest that these parapatric TULV clades represent functionally distinct, incipient species, which are likely differently affected by genetic polymorphisms in the host. This highlights the potential of natural viral contact zones as systems for investigating the genetic and evolutionary factors enabling or restricting the transmission of RNA viruses. Article in Journal/Newspaper Common vole Microtus arvalis Oxford University Press Tula ENVELOPE(-65.650,-65.650,-65.517,-65.517) Virus Evolution |
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Open Polar |
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Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
topic |
Virology Microbiology |
spellingShingle |
Virology Microbiology Labutin, Anton Heckel, Gerald Genome-wide support for incipient Tula hantavirus species within a single rodent host lineage |
topic_facet |
Virology Microbiology |
description |
Abstract Evolutionary divergence of viruses is most commonly driven by co-divergence with their hosts or through isolation of transmission after host shifts. It remains mostly unknown, however, whether divergent phylogenetic clades within named virus species represent functionally equivalent byproducts of high evolutionary rates or rather incipient virus species. Here, we test these alternatives with genomic data from two widespread phylogenetic clades in Tula orthohantavirus (TULV) within a single evolutionary lineage of their natural rodent host, the common vole Microtus arvalis. We examined voles from forty-two locations in the contact region between clades for TULV infection by reverse transcription (RT)-PCR. Sequencing yielded twenty-three TULV Central North and twenty-one TULV Central South genomes, which differed by 14.9–18.5 per cent at the nucleotide and 2.2–3.7 per cent at the amino acid (AA) level without evidence of recombination or reassortment between clades. Geographic cline analyses demonstrated an abrupt (<1 km wide) transition between the parapatric TULV clades in continuous landscape. This transition was located within the Central mitochondrial lineage of M. arvalis, and genomic single nucleotide polymorphisms showed gradual mixing of host populations across it. Genomic differentiation of hosts was much weaker across the TULV Central North to South transition than across the nearby hybrid zone between two evolutionary lineages in the host. We suggest that these parapatric TULV clades represent functionally distinct, incipient species, which are likely differently affected by genetic polymorphisms in the host. This highlights the potential of natural viral contact zones as systems for investigating the genetic and evolutionary factors enabling or restricting the transmission of RNA viruses. |
author2 |
Swiss National Science Foundation Swiss National Science Foundation |
format |
Article in Journal/Newspaper |
author |
Labutin, Anton Heckel, Gerald |
author_facet |
Labutin, Anton Heckel, Gerald |
author_sort |
Labutin, Anton |
title |
Genome-wide support for incipient Tula hantavirus species within a single rodent host lineage |
title_short |
Genome-wide support for incipient Tula hantavirus species within a single rodent host lineage |
title_full |
Genome-wide support for incipient Tula hantavirus species within a single rodent host lineage |
title_fullStr |
Genome-wide support for incipient Tula hantavirus species within a single rodent host lineage |
title_full_unstemmed |
Genome-wide support for incipient Tula hantavirus species within a single rodent host lineage |
title_sort |
genome-wide support for incipient tula hantavirus species within a single rodent host lineage |
publisher |
Oxford University Press (OUP) |
publishDate |
2024 |
url |
http://dx.doi.org/10.1093/ve/veae002 https://academic.oup.com/ve/advance-article-pdf/doi/10.1093/ve/veae002/55120087/veae002.pdf https://academic.oup.com/ve/article-pdf/10/1/veae002/56487491/veae002.pdf |
long_lat |
ENVELOPE(-65.650,-65.650,-65.517,-65.517) |
geographic |
Tula |
geographic_facet |
Tula |
genre |
Common vole Microtus arvalis |
genre_facet |
Common vole Microtus arvalis |
op_source |
Virus Evolution volume 10, issue 1 ISSN 2057-1577 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/ve/veae002 |
container_title |
Virus Evolution |
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1792499161543737344 |