Genetic diversity and connectivity of the Ostreid herpesvirus 1 populations in France: A first attempt to phylogeographic inference for a marine mollusc disease
Abstract The genetic diversity of viral populations is a key driver of the spatial and temporal diffusion of viruses; yet, studying the diversity of whole genomes from natural populations still remains a challenge. Phylodynamic approaches are commonly used for RNA viruses harboring small genomes but...
Published in: | Virus Evolution |
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Oxford University Press (OUP)
2022
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Online Access: | http://dx.doi.org/10.1093/ve/veac039 https://academic.oup.com/ve/advance-article-pdf/doi/10.1093/ve/veac039/43431704/veac039.pdf https://academic.oup.com/ve/article-pdf/8/1/veac039/43771399/veac039.pdf |
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croxfordunivpr:10.1093/ve/veac039 2024-10-13T14:10:08+00:00 Genetic diversity and connectivity of the Ostreid herpesvirus 1 populations in France: A first attempt to phylogeographic inference for a marine mollusc disease Delmotte, Jean Pelletier, Camille Morga, Benjamin Galinier, Richard Petton, Bruno Lamy, Jean-Baptiste Kaltz, Oliver Avarre, Jean-Christophe Jacquot, Maude Montagnani, Caroline Escoubas, Jean-Michel Institut Français de Recherche pour l’Exploitation de la Mer VIVALDI Fond Européen pour les Affaires Maritimes et la Pêche Centre Méditerranéen de l’Environnement et de la Biodiversité 2022 http://dx.doi.org/10.1093/ve/veac039 https://academic.oup.com/ve/advance-article-pdf/doi/10.1093/ve/veac039/43431704/veac039.pdf https://academic.oup.com/ve/article-pdf/8/1/veac039/43771399/veac039.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ Virus Evolution volume 8, issue 1 ISSN 2057-1577 journal-article 2022 croxfordunivpr https://doi.org/10.1093/ve/veac039 2024-09-17T04:29:59Z Abstract The genetic diversity of viral populations is a key driver of the spatial and temporal diffusion of viruses; yet, studying the diversity of whole genomes from natural populations still remains a challenge. Phylodynamic approaches are commonly used for RNA viruses harboring small genomes but have only rarely been applied to DNA viruses with larger genomes. Here, we used the Pacific oyster mortality syndrome (a disease that affects oyster farms around the world) as a model to study the genetic diversity of its causative agent, the Ostreid herpesvirus 1 (OsHV-1) in the three main French oyster-farming areas. Using ultra-deep sequencing on individual moribund oysters and an innovative combination of bioinformatics tools, we de novo assembled twenty-one OsHV-1 new genomes. Combining quantification of major and minor genetic variations, phylogenetic analysis, and ancestral state reconstruction of discrete traits approaches, we assessed the connectivity of OsHV-1 viral populations between the three oyster-farming areas. Our results suggest that the Marennes-Oléron Bay represents the main source of OsHV-1 diversity, from where the virus has dispersed to other farming areas, a scenario consistent with current practices of oyster transfers in France. We demonstrate that phylodynamic approaches can be applied to aquatic DNA viruses to determine how epidemiological, immunological, and evolutionary processes act and potentially interact to shape their diversity patterns. Article in Journal/Newspaper Pacific oyster Oxford University Press Pacific Virus Evolution 8 1 |
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Open Polar |
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Oxford University Press |
op_collection_id |
croxfordunivpr |
language |
English |
description |
Abstract The genetic diversity of viral populations is a key driver of the spatial and temporal diffusion of viruses; yet, studying the diversity of whole genomes from natural populations still remains a challenge. Phylodynamic approaches are commonly used for RNA viruses harboring small genomes but have only rarely been applied to DNA viruses with larger genomes. Here, we used the Pacific oyster mortality syndrome (a disease that affects oyster farms around the world) as a model to study the genetic diversity of its causative agent, the Ostreid herpesvirus 1 (OsHV-1) in the three main French oyster-farming areas. Using ultra-deep sequencing on individual moribund oysters and an innovative combination of bioinformatics tools, we de novo assembled twenty-one OsHV-1 new genomes. Combining quantification of major and minor genetic variations, phylogenetic analysis, and ancestral state reconstruction of discrete traits approaches, we assessed the connectivity of OsHV-1 viral populations between the three oyster-farming areas. Our results suggest that the Marennes-Oléron Bay represents the main source of OsHV-1 diversity, from where the virus has dispersed to other farming areas, a scenario consistent with current practices of oyster transfers in France. We demonstrate that phylodynamic approaches can be applied to aquatic DNA viruses to determine how epidemiological, immunological, and evolutionary processes act and potentially interact to shape their diversity patterns. |
author2 |
Institut Français de Recherche pour l’Exploitation de la Mer VIVALDI Fond Européen pour les Affaires Maritimes et la Pêche Centre Méditerranéen de l’Environnement et de la Biodiversité |
format |
Article in Journal/Newspaper |
author |
Delmotte, Jean Pelletier, Camille Morga, Benjamin Galinier, Richard Petton, Bruno Lamy, Jean-Baptiste Kaltz, Oliver Avarre, Jean-Christophe Jacquot, Maude Montagnani, Caroline Escoubas, Jean-Michel |
spellingShingle |
Delmotte, Jean Pelletier, Camille Morga, Benjamin Galinier, Richard Petton, Bruno Lamy, Jean-Baptiste Kaltz, Oliver Avarre, Jean-Christophe Jacquot, Maude Montagnani, Caroline Escoubas, Jean-Michel Genetic diversity and connectivity of the Ostreid herpesvirus 1 populations in France: A first attempt to phylogeographic inference for a marine mollusc disease |
author_facet |
Delmotte, Jean Pelletier, Camille Morga, Benjamin Galinier, Richard Petton, Bruno Lamy, Jean-Baptiste Kaltz, Oliver Avarre, Jean-Christophe Jacquot, Maude Montagnani, Caroline Escoubas, Jean-Michel |
author_sort |
Delmotte, Jean |
title |
Genetic diversity and connectivity of the Ostreid herpesvirus 1 populations in France: A first attempt to phylogeographic inference for a marine mollusc disease |
title_short |
Genetic diversity and connectivity of the Ostreid herpesvirus 1 populations in France: A first attempt to phylogeographic inference for a marine mollusc disease |
title_full |
Genetic diversity and connectivity of the Ostreid herpesvirus 1 populations in France: A first attempt to phylogeographic inference for a marine mollusc disease |
title_fullStr |
Genetic diversity and connectivity of the Ostreid herpesvirus 1 populations in France: A first attempt to phylogeographic inference for a marine mollusc disease |
title_full_unstemmed |
Genetic diversity and connectivity of the Ostreid herpesvirus 1 populations in France: A first attempt to phylogeographic inference for a marine mollusc disease |
title_sort |
genetic diversity and connectivity of the ostreid herpesvirus 1 populations in france: a first attempt to phylogeographic inference for a marine mollusc disease |
publisher |
Oxford University Press (OUP) |
publishDate |
2022 |
url |
http://dx.doi.org/10.1093/ve/veac039 https://academic.oup.com/ve/advance-article-pdf/doi/10.1093/ve/veac039/43431704/veac039.pdf https://academic.oup.com/ve/article-pdf/8/1/veac039/43771399/veac039.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Virus Evolution volume 8, issue 1 ISSN 2057-1577 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/ve/veac039 |
container_title |
Virus Evolution |
container_volume |
8 |
container_issue |
1 |
_version_ |
1812817286343426048 |