Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport
Abstract Water uptake by roots is a key adaptation of plants to aerial life. Water uptake depends on root system architecture (RSA) and tissue hydraulic properties that, together, shape the root hydraulic architecture. This work investigates how the interplay between conductivities along radial (e.g...
Published in: | Plant Physiology |
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Oxford University Press (OUP)
2022
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Online Access: | http://dx.doi.org/10.1093/plphys/kiac281 https://academic.oup.com/plphys/advance-article-pdf/doi/10.1093/plphys/kiac281/44145584/kiac281.pdf https://academic.oup.com/plphys/article-pdf/190/2/1289/46114103/kiac281.pdf |
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croxfordunivpr:10.1093/plphys/kiac281 2024-05-19T07:39:46+00:00 Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport Boursiac, Yann Pradal, Christophe Bauget, Fabrice Lucas, Mikaël Delivorias, Stathis Godin, Christophe Maurel, Christophe Agence Nationale de la Recherche European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program 2022 http://dx.doi.org/10.1093/plphys/kiac281 https://academic.oup.com/plphys/advance-article-pdf/doi/10.1093/plphys/kiac281/44145584/kiac281.pdf https://academic.oup.com/plphys/article-pdf/190/2/1289/46114103/kiac281.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by-nc-nd/4.0/ Plant Physiology volume 190, issue 2, page 1289-1306 ISSN 0032-0889 1532-2548 journal-article 2022 croxfordunivpr https://doi.org/10.1093/plphys/kiac281 2024-05-02T09:32:04Z Abstract Water uptake by roots is a key adaptation of plants to aerial life. Water uptake depends on root system architecture (RSA) and tissue hydraulic properties that, together, shape the root hydraulic architecture. This work investigates how the interplay between conductivities along radial (e.g. aquaporins) and axial (e.g. xylem vessels) pathways determines the water transport properties of highly branched RSAs as found in adult Arabidopsis (Arabidopsis thaliana) plants. A hydraulic model named HydroRoot was developed, based on multi-scale tree graph representations of RSAs. Root water flow was measured by the pressure chamber technique after successive cuts of a same root system from the tip toward the base. HydroRoot model inversion in corresponding RSAs allowed us to concomitantly determine radial and axial conductivities, providing evidence that the latter is often overestimated by classical evaluation based on the Hagen–Poiseuille law. Organizing principles of Arabidopsis primary and lateral root growth and branching were determined and used to apply the HydroRoot model to an extended set of simulated RSAs. Sensitivity analyses revealed that water transport can be co-limited by radial and axial conductances throughout the whole RSA. The number of roots that can be sectioned (intercepted) at a given distance from the base was defined as an accessible and informative indicator of RSA. The overall set of experimental and theoretical procedures was applied to plants mutated in ESKIMO1 and previously shown to have xylem collapse. This approach will be instrumental to dissect the root water transport phenotype of plants with intricate alterations in root growth or transport functions. Article in Journal/Newspaper eskimo* Oxford University Press Plant Physiology |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Water uptake by roots is a key adaptation of plants to aerial life. Water uptake depends on root system architecture (RSA) and tissue hydraulic properties that, together, shape the root hydraulic architecture. This work investigates how the interplay between conductivities along radial (e.g. aquaporins) and axial (e.g. xylem vessels) pathways determines the water transport properties of highly branched RSAs as found in adult Arabidopsis (Arabidopsis thaliana) plants. A hydraulic model named HydroRoot was developed, based on multi-scale tree graph representations of RSAs. Root water flow was measured by the pressure chamber technique after successive cuts of a same root system from the tip toward the base. HydroRoot model inversion in corresponding RSAs allowed us to concomitantly determine radial and axial conductivities, providing evidence that the latter is often overestimated by classical evaluation based on the Hagen–Poiseuille law. Organizing principles of Arabidopsis primary and lateral root growth and branching were determined and used to apply the HydroRoot model to an extended set of simulated RSAs. Sensitivity analyses revealed that water transport can be co-limited by radial and axial conductances throughout the whole RSA. The number of roots that can be sectioned (intercepted) at a given distance from the base was defined as an accessible and informative indicator of RSA. The overall set of experimental and theoretical procedures was applied to plants mutated in ESKIMO1 and previously shown to have xylem collapse. This approach will be instrumental to dissect the root water transport phenotype of plants with intricate alterations in root growth or transport functions. |
author2 |
Agence Nationale de la Recherche European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program |
format |
Article in Journal/Newspaper |
author |
Boursiac, Yann Pradal, Christophe Bauget, Fabrice Lucas, Mikaël Delivorias, Stathis Godin, Christophe Maurel, Christophe |
spellingShingle |
Boursiac, Yann Pradal, Christophe Bauget, Fabrice Lucas, Mikaël Delivorias, Stathis Godin, Christophe Maurel, Christophe Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
author_facet |
Boursiac, Yann Pradal, Christophe Bauget, Fabrice Lucas, Mikaël Delivorias, Stathis Godin, Christophe Maurel, Christophe |
author_sort |
Boursiac, Yann |
title |
Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
title_short |
Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
title_full |
Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
title_fullStr |
Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
title_full_unstemmed |
Phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
title_sort |
phenotyping and modeling of root hydraulic architecture reveal critical determinants of axial water transport |
publisher |
Oxford University Press (OUP) |
publishDate |
2022 |
url |
http://dx.doi.org/10.1093/plphys/kiac281 https://academic.oup.com/plphys/advance-article-pdf/doi/10.1093/plphys/kiac281/44145584/kiac281.pdf https://academic.oup.com/plphys/article-pdf/190/2/1289/46114103/kiac281.pdf |
genre |
eskimo* |
genre_facet |
eskimo* |
op_source |
Plant Physiology volume 190, issue 2, page 1289-1306 ISSN 0032-0889 1532-2548 |
op_rights |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1093/plphys/kiac281 |
container_title |
Plant Physiology |
_version_ |
1799479357449699328 |