Poaceae Type II Galactinol Synthase 2 from Antarctic Flowering Plant Deschampsia antarctica and Rice Improves Cold and Drought Tolerance by Accumulation of Raffinose Family Oligosaccharides in Transgenic Rice Plants
Abstract Deschampsia antarctica is a Poaceae grass that has adapted to and colonized Antarctica. When D. antarctica plants were subjected to cold and dehydration stress both in the Antarctic field and in laboratory experiments, galactinol, a precursor of raffinose family oligosaccharides (RFOs) and...
Published in: | Plant and Cell Physiology |
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Oxford University Press (OUP)
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Online Access: | http://dx.doi.org/10.1093/pcp/pcz180 http://academic.oup.com/pcp/advance-article-pdf/doi/10.1093/pcp/pcz180/30123552/pcz180.pdf http://academic.oup.com/pcp/article-pdf/61/1/88/32500138/pcz180.pdf |
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croxfordunivpr:10.1093/pcp/pcz180 2024-09-15T17:48:20+00:00 Poaceae Type II Galactinol Synthase 2 from Antarctic Flowering Plant Deschampsia antarctica and Rice Improves Cold and Drought Tolerance by Accumulation of Raffinose Family Oligosaccharides in Transgenic Rice Plants Cui, Li Hua Byun, Mi Young Oh, Hyeong Geun Kim, Sung Jin Lee, Jungeun Park, Hyun Lee, Hyoungseok Kim, Woo Taek The Polar Genomics 101 Project: Genome analysis Korea Polar Research Institute Next-Generation BioGreen 21 Program for Agriculture and Technology Development Rural Development Administration and the Basic Science Research Program National Research Foundation NRF Ministry of Education, Republic of Korea 2019 http://dx.doi.org/10.1093/pcp/pcz180 http://academic.oup.com/pcp/advance-article-pdf/doi/10.1093/pcp/pcz180/30123552/pcz180.pdf http://academic.oup.com/pcp/article-pdf/61/1/88/32500138/pcz180.pdf en eng Oxford University Press (OUP) https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model Plant and Cell Physiology volume 61, issue 1, page 88-104 ISSN 0032-0781 1471-9053 journal-article 2019 croxfordunivpr https://doi.org/10.1093/pcp/pcz180 2024-08-05T04:32:30Z Abstract Deschampsia antarctica is a Poaceae grass that has adapted to and colonized Antarctica. When D. antarctica plants were subjected to cold and dehydration stress both in the Antarctic field and in laboratory experiments, galactinol, a precursor of raffinose family oligosaccharides (RFOs) and raffinose were highly accumulated, which was accompanied by upregulation of galactinol synthase (GolS). The Poaceae monocots have a small family of GolS genes, which are divided into two distinct groups called types I and II. Type II GolSs are highly expanded in cold-adapted monocot plants. Transgenic rice plants, in which type II D. antarctica GolS2 (DaGolS2) and rice GolS2 (OsGolS2) were constitutively expressed, were markedly tolerant to cold and drought stress as compared to the wild-type rice plants. The RFO contents and GolS enzyme activities were higher in the DaGolS2- and OsGolS2-overexpressing progeny than in the wild-type plants under both normal and stress conditions. DaGolS2 and OsGolS2 overexpressors contained reduced levels of reactive oxygen species (ROS) relative to the wild-type plants after cold and drought treatments. Overall, these results suggest that Poaceae type II GolS2s play a conserved role in D. antarctica and rice in response to drought and cold stress by inducing the accumulation of RFO and decreasing ROS levels. Article in Journal/Newspaper Antarc* Antarctic Antarctica Oxford University Press Plant and Cell Physiology 61 1 88 104 |
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Open Polar |
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Oxford University Press |
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croxfordunivpr |
language |
English |
description |
Abstract Deschampsia antarctica is a Poaceae grass that has adapted to and colonized Antarctica. When D. antarctica plants were subjected to cold and dehydration stress both in the Antarctic field and in laboratory experiments, galactinol, a precursor of raffinose family oligosaccharides (RFOs) and raffinose were highly accumulated, which was accompanied by upregulation of galactinol synthase (GolS). The Poaceae monocots have a small family of GolS genes, which are divided into two distinct groups called types I and II. Type II GolSs are highly expanded in cold-adapted monocot plants. Transgenic rice plants, in which type II D. antarctica GolS2 (DaGolS2) and rice GolS2 (OsGolS2) were constitutively expressed, were markedly tolerant to cold and drought stress as compared to the wild-type rice plants. The RFO contents and GolS enzyme activities were higher in the DaGolS2- and OsGolS2-overexpressing progeny than in the wild-type plants under both normal and stress conditions. DaGolS2 and OsGolS2 overexpressors contained reduced levels of reactive oxygen species (ROS) relative to the wild-type plants after cold and drought treatments. Overall, these results suggest that Poaceae type II GolS2s play a conserved role in D. antarctica and rice in response to drought and cold stress by inducing the accumulation of RFO and decreasing ROS levels. |
author2 |
The Polar Genomics 101 Project: Genome analysis Korea Polar Research Institute Next-Generation BioGreen 21 Program for Agriculture and Technology Development Rural Development Administration and the Basic Science Research Program National Research Foundation NRF Ministry of Education, Republic of Korea |
format |
Article in Journal/Newspaper |
author |
Cui, Li Hua Byun, Mi Young Oh, Hyeong Geun Kim, Sung Jin Lee, Jungeun Park, Hyun Lee, Hyoungseok Kim, Woo Taek |
spellingShingle |
Cui, Li Hua Byun, Mi Young Oh, Hyeong Geun Kim, Sung Jin Lee, Jungeun Park, Hyun Lee, Hyoungseok Kim, Woo Taek Poaceae Type II Galactinol Synthase 2 from Antarctic Flowering Plant Deschampsia antarctica and Rice Improves Cold and Drought Tolerance by Accumulation of Raffinose Family Oligosaccharides in Transgenic Rice Plants |
author_facet |
Cui, Li Hua Byun, Mi Young Oh, Hyeong Geun Kim, Sung Jin Lee, Jungeun Park, Hyun Lee, Hyoungseok Kim, Woo Taek |
author_sort |
Cui, Li Hua |
title |
Poaceae Type II Galactinol Synthase 2 from Antarctic Flowering Plant Deschampsia antarctica and Rice Improves Cold and Drought Tolerance by Accumulation of Raffinose Family Oligosaccharides in Transgenic Rice Plants |
title_short |
Poaceae Type II Galactinol Synthase 2 from Antarctic Flowering Plant Deschampsia antarctica and Rice Improves Cold and Drought Tolerance by Accumulation of Raffinose Family Oligosaccharides in Transgenic Rice Plants |
title_full |
Poaceae Type II Galactinol Synthase 2 from Antarctic Flowering Plant Deschampsia antarctica and Rice Improves Cold and Drought Tolerance by Accumulation of Raffinose Family Oligosaccharides in Transgenic Rice Plants |
title_fullStr |
Poaceae Type II Galactinol Synthase 2 from Antarctic Flowering Plant Deschampsia antarctica and Rice Improves Cold and Drought Tolerance by Accumulation of Raffinose Family Oligosaccharides in Transgenic Rice Plants |
title_full_unstemmed |
Poaceae Type II Galactinol Synthase 2 from Antarctic Flowering Plant Deschampsia antarctica and Rice Improves Cold and Drought Tolerance by Accumulation of Raffinose Family Oligosaccharides in Transgenic Rice Plants |
title_sort |
poaceae type ii galactinol synthase 2 from antarctic flowering plant deschampsia antarctica and rice improves cold and drought tolerance by accumulation of raffinose family oligosaccharides in transgenic rice plants |
publisher |
Oxford University Press (OUP) |
publishDate |
2019 |
url |
http://dx.doi.org/10.1093/pcp/pcz180 http://academic.oup.com/pcp/advance-article-pdf/doi/10.1093/pcp/pcz180/30123552/pcz180.pdf http://academic.oup.com/pcp/article-pdf/61/1/88/32500138/pcz180.pdf |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Plant and Cell Physiology volume 61, issue 1, page 88-104 ISSN 0032-0781 1471-9053 |
op_rights |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
op_doi |
https://doi.org/10.1093/pcp/pcz180 |
container_title |
Plant and Cell Physiology |
container_volume |
61 |
container_issue |
1 |
container_start_page |
88 |
op_container_end_page |
104 |
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1810289483025743872 |