Horizontal gene transfer as a mechanism for the promiscuous acquisition of distinct classes of IRES by avian caliciviruses
Abstract In contrast to members of Picornaviridae which have long 5′-untranslated regions (5′UTRs) containing internal ribosomal entry sites (IRESs) that form five distinct classes, members of Caliciviridae typically have short 5′UTRs and initiation of translation on them is mediated by interaction...
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Online Access: | http://dx.doi.org/10.1093/nar/gkab1243 https://academic.oup.com/nar/article-pdf/50/2/1052/42269978/gkab1243.pdf |
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croxfordunivpr:10.1093/nar/gkab1243 2024-05-19T07:47:51+00:00 Horizontal gene transfer as a mechanism for the promiscuous acquisition of distinct classes of IRES by avian caliciviruses Arhab, Yani Miścicka, Anna Pestova, Tatyana V Hellen, Christopher U T National Institutes of Health 2021 http://dx.doi.org/10.1093/nar/gkab1243 https://academic.oup.com/nar/article-pdf/50/2/1052/42269978/gkab1243.pdf en eng Oxford University Press (OUP) https://creativecommons.org/licenses/by/4.0/ Nucleic Acids Research volume 50, issue 2, page 1052-1068 ISSN 0305-1048 1362-4962 journal-article 2021 croxfordunivpr https://doi.org/10.1093/nar/gkab1243 2024-04-25T07:57:17Z Abstract In contrast to members of Picornaviridae which have long 5′-untranslated regions (5′UTRs) containing internal ribosomal entry sites (IRESs) that form five distinct classes, members of Caliciviridae typically have short 5′UTRs and initiation of translation on them is mediated by interaction of the viral 5′-terminal genome-linked protein (VPg) with subunits of eIF4F rather than by an IRES. The recent description of calicivirus genomes with 500–900nt long 5′UTRs was therefore unexpected and prompted us to examine them in detail. Sequence analysis and structural modelling of the atypically long 5′UTRs of Caliciviridae sp. isolate yc-13 and six other caliciviruses suggested that they contain picornavirus-like type 2 IRESs, whereas ruddy turnstone calicivirus (RTCV) and Caliciviridae sp. isolate hwf182cal1 calicivirus contain type 4 and type 5 IRESs, respectively. The suggestion that initiation on RTCV mRNA occurs by the type 4 IRES mechanism was confirmed experimentally using in vitro reconstitution. The high sequence identity between identified calicivirus IRESs and specific picornavirus IRESs suggests a common evolutionary origin. These calicivirus IRESs occur in a single phylogenetic branch of Caliciviridae and were likely acquired by horizontal gene transfer. Article in Journal/Newspaper Ruddy Turnstone Oxford University Press Nucleic Acids Research |
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Abstract In contrast to members of Picornaviridae which have long 5′-untranslated regions (5′UTRs) containing internal ribosomal entry sites (IRESs) that form five distinct classes, members of Caliciviridae typically have short 5′UTRs and initiation of translation on them is mediated by interaction of the viral 5′-terminal genome-linked protein (VPg) with subunits of eIF4F rather than by an IRES. The recent description of calicivirus genomes with 500–900nt long 5′UTRs was therefore unexpected and prompted us to examine them in detail. Sequence analysis and structural modelling of the atypically long 5′UTRs of Caliciviridae sp. isolate yc-13 and six other caliciviruses suggested that they contain picornavirus-like type 2 IRESs, whereas ruddy turnstone calicivirus (RTCV) and Caliciviridae sp. isolate hwf182cal1 calicivirus contain type 4 and type 5 IRESs, respectively. The suggestion that initiation on RTCV mRNA occurs by the type 4 IRES mechanism was confirmed experimentally using in vitro reconstitution. The high sequence identity between identified calicivirus IRESs and specific picornavirus IRESs suggests a common evolutionary origin. These calicivirus IRESs occur in a single phylogenetic branch of Caliciviridae and were likely acquired by horizontal gene transfer. |
author2 |
National Institutes of Health |
format |
Article in Journal/Newspaper |
author |
Arhab, Yani Miścicka, Anna Pestova, Tatyana V Hellen, Christopher U T |
spellingShingle |
Arhab, Yani Miścicka, Anna Pestova, Tatyana V Hellen, Christopher U T Horizontal gene transfer as a mechanism for the promiscuous acquisition of distinct classes of IRES by avian caliciviruses |
author_facet |
Arhab, Yani Miścicka, Anna Pestova, Tatyana V Hellen, Christopher U T |
author_sort |
Arhab, Yani |
title |
Horizontal gene transfer as a mechanism for the promiscuous acquisition of distinct classes of IRES by avian caliciviruses |
title_short |
Horizontal gene transfer as a mechanism for the promiscuous acquisition of distinct classes of IRES by avian caliciviruses |
title_full |
Horizontal gene transfer as a mechanism for the promiscuous acquisition of distinct classes of IRES by avian caliciviruses |
title_fullStr |
Horizontal gene transfer as a mechanism for the promiscuous acquisition of distinct classes of IRES by avian caliciviruses |
title_full_unstemmed |
Horizontal gene transfer as a mechanism for the promiscuous acquisition of distinct classes of IRES by avian caliciviruses |
title_sort |
horizontal gene transfer as a mechanism for the promiscuous acquisition of distinct classes of ires by avian caliciviruses |
publisher |
Oxford University Press (OUP) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1093/nar/gkab1243 https://academic.oup.com/nar/article-pdf/50/2/1052/42269978/gkab1243.pdf |
genre |
Ruddy Turnstone |
genre_facet |
Ruddy Turnstone |
op_source |
Nucleic Acids Research volume 50, issue 2, page 1052-1068 ISSN 0305-1048 1362-4962 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/nar/gkab1243 |
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Nucleic Acids Research |
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